Synergism of sophoraflavanone G with norfloxacin against effluxing antibiotic-resistant Staphylococcus aureus

Sun, Zhong-Lin; Sun, Shi-Chang; He, Jian-Ming; Lan, Jiang-Er; Gibbons, Simon; Mu, Quanquan

doi:10.1016/j.ijantimicag.2020.106098

Cited by 26 publications

(18 citation statements)

References 20 publications

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“…For example, the study of de Morais Oliveira-Tintino et al [77] revealed that 1,8-naphthyridine sulfonamide derivatives possessed potential Tet(K) and MsrA efflux pump inhibitory activities. In addition, the natural product, sophoraflavanone G showed a potential in vitro and in vivo antibacterial activity and can be used as antibiotic-potentiating agent against S. aureus [78]. Moreover, the computational study of Ghosh et al [79] suggested that cathinone had a good electrophilicity and ionization potential which enabled it to interact with most efflux pumps.…”

Section: Plos Onementioning

confidence: 99%

Promising FDA-approved drugs with efflux pump inhibitory activities against clinical isolates of Staphylococcus aureus

et al. 2022

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Background and objectives Staphylococcus aureus is an opportunistic pathogen that causes wide range of nosocomial and community-acquired infections which have spread worldwide leading to an urgent need for developing effective anti-staphylococcal agents. Efflux is an important resistance mechanism that bacteria used to fight the antimicrobial action. This study aimed to investigate the efflux mechanism in S. aureus and assess diclofenac, domperidone, glyceryl trinitrate and metformin as potential efflux pump inhibitors that can be used in combination with antibiotics for treating topical infections caused by S. aureus. Materials and methods Efflux was detected qualitatively by the ethidium bromide Cart-Wheel method followed by investigating the presence of efflux genes by polymerase chain reaction. Twenty-six isolates were selected for further investigation of efflux by Cart-Wheel method in absence and presence of tested compounds followed by quantitative efflux assay. Furthermore, antibiotics minimum inhibitory concentrations in absence and presence of tested compounds were determined. The effects of tested drugs on expression levels of efflux genes norA, fexA and tetK were determined by quantitative real time-polymerase chain reaction. Results Efflux was found in 65.3% of isolates, the prevalence of norA, tetK, fexA and msrA genes were 91.7%, 77.8%, 27.8% and 6.9%. Efflux assay revealed that tested drugs had potential efflux inhibitory activities, reduced the antibiotic’s MICs and significantly decreased the relative expression of efflux genes. Conclusion Diclofenac sodium, domperidone and glyceryl trinitrate showed higher efflux inhibitory activities than verapamil and metformin. To our knowledge, this is the first report that shows that diclofenac sodium, glyceryl trinitrate and domperidone have efflux pump inhibitory activities against S. aureus.

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Section: Plos Onementioning

confidence: 99%

Promising FDA-approved drugs with efflux pump inhibitory activities against clinical isolates of Staphylococcus aureus

et al. 2022

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“…To further investigate whether K 4 or K 5 reverse the occurrence of bacterial drug resistance by affecting the drug efflux, the chemical sensitization assay with minor modifications was performed. [36,37] Here, the substrate of efflux pump ethidium bromide (EtBr) was used as indicators of the efflux pump inhibition, [38] and the efflux pump inhibitor carbonyl cyanide 3-chlorobenzene hydrazone (CCCP) was used as a positive control. [39] MICs of EtBr and CCCP in mixtures of K 4 or K 5 and traditional antibiotics were determined according to the above methods of the sensitization test with different final concentration ratios.…”

Section: Sensitization Testmentioning

confidence: 99%

Antimicrobial peptides–antibiotics combination: An effective strategy targeting drug‐resistant Gram‐negative bacteria

et al. 2022

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To address the growing resistance of Gram-negative bacteria and enhance the clinical application potential of antimicrobial peptides. In this study, we combined two α-helical antimicrobial peptides K 4 (WRKWRKWRKWRK-NH 2 ) or K 5 (WRKWR KWRKWRKWRK-NH 2 ) with four traditional antibiotics (gentamicin, rifampicin, ciprofloxacin, and imipenem). Then we explored their potential and mechanism in inhibiting the generation of bacterial resistance. After adding K 4 or K 5 , the tendency to produce resistant bacteria from traditional antibiotics declined significantly. And the obtained antibiotics-resistant bacteria were further re-sensitive to their corresponding traditional antibiotics with the addition of K 4 or K 5 . Mostly, the combination showed synergistic or additive antibacterial effects on both the standard strains and the obtained antibiotics-resistant bacteria, with a much better effect on the latter. Chemical sensitization and the outer membrane permeability experiments demonstrated that K 4 and K 5 may influence the drug efflux of the tested bacteria, while at the same time improving the outer membrane permeability of the obtained antibiotics-resistant bacteria to traditional antibiotics by acting as a film breaker.They ultimately eliminated the generation of drug resistance. These results provided an effort that could break Gram-negative bacteria's resistance.

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“…Other examples of efflux pump inhibitors are sophoroflavone G, jatrorrhizine, isovaleryl shikonin, griseviridin, 2-(2-aminophenyl)indole, flavonoids, essential oils and several plant extracts. It should be mentioned that both berberine and jatrorrhizine have also been isolated from Mahonia bealei, together with a variety of other alkaloids, terpenoids and polyphenols, and synergistic interactions with CAAs can be an expected result [43,44,45,46,47,48,40,49,50,51,52,53].…”

Section: Origin and Type Of The Natural Productsmentioning

confidence: 99%

Perspectives on the enhancement of commercially available antibiotics by natural products

Nitsch-Velasquez

Segura²,

Barrios

et al. 2022

Preprint

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Background: Opportunistic resistant bacteria are health and economically relevant in the health care systems and in industries worldwide, especially in the so-called resistant bacteria era (RBE). Enhancing the activity of commercially available antibiotics (CAAs) with di erent types of natural products (NPs) is a successful antimicrobial strategy, for instance the amoxicillin and clavulanate mixture. Objective: To nd research trends in this eld during 2015-2020 and to detect potential drug hits with potential to diversify formulations and materials design that can be useful to manage the RBE. Systematic review results: It yielded 190 reports of synergistic e ects of CAAs and NPs. The analyzed variables were: a) natural products origin: plant family, genera, secondary metabolite type; b) strains: +/- Gram, genera, most frequent species, application eld; and c) CAAs: family, most frequent CAAs. The families with potential to have more bioactive species were Apocynaceae, Rubiaceae, Euphorbiaceae (Isbio factor). Lonicera had the highest reports amount. Polyphenols and avonoids were the majority of pure NPs tested. Several potential drug hits for antibiotic activity enhancement at synergistic level were identi ed together with potential mechanisms of action: berberine (drug e ux inhibitor{DEI, bio lm inhibitor{BI), curcumin (BI), essential oils (BI), 3-o-metyl-butylgallato (inhibition of fatty acid saturation), among others. About the half of the tested strains were gram positive, being Methicillin Resistant Staphylococcus aureus (MRSA) the most frequently tested. Escherichia coli was the gram negative strain most frequently reported, including enterotoxigenic and extended spectrum beta-lactamases producers. The growth of other foodborne genera strains, such as Listeria and Salmonella, were also inhibited. Aminoglycosides were the family most reported, with gentamicin as the most commonly studied. Conclusions: NPs as either as plant extracts from a variety of families, or as puri ed compounds specially avonoids and polyphenols, have shown e ective results to enhance the antibiotic activity of CAAs against gram positive and negative strains relevant to HC and FI. Their mechanisms of action are starting to be determined, as the case EPIs and BIs. Further research is needed to achieve co-formulations and materials design useful for those elds, that can certainly be positively impacted by pursuing this strategy.

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Synergism of sophoraflavanone G with norfloxacin against effluxing antibiotic-resistant Staphylococcus aureus

Cited by 26 publications

References 20 publications

Promising FDA-approved drugs with efflux pump inhibitory activities against clinical isolates of Staphylococcus aureus

Promising FDA-approved drugs with efflux pump inhibitory activities against clinical isolates of Staphylococcus aureus

Antimicrobial peptides–antibiotics combination: An effective strategy targeting drug‐resistant Gram‐negative bacteria

Perspectives on the enhancement of commercially available antibiotics by natural products

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