Synergisms in alpha-glucosidase inhibition and antioxidant activity of Camellia sinensis L. Kuntze and Eugenia uniflora L. Ethanolic Extracts

Vinholes, Juliana; Vizzotto, M.

doi:10.4103/0974-8490.197797

Cited by 39 publications

(22 citation statements)

References 42 publications

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“…18 Furthermore, dissimilarities in antioxidant activity and the yield of phenolic compounds have been testified to be influenced by extracting solvents. 19 Considering the variable results which can be obtained during extracting of antioxidants using various solvents, it is quite difficult to develop a standard extraction protocol that can provide better or more consistent results. From the toxicological point of view water is considered to be safer than methanol, 20 particularly with regard to applications or use in the food industries.…”

Section: Effects Of the Solvent Systemmentioning

confidence: 99%

Total Phenolic, Flavonoid and Alkaloid Contents, Oxidative DNA Damage Protective and Antioxidant Properties of Methanol and Aqueous Extracts of Dissotis rotundifolia Whole Plant

Darkwah

Adinortey

et al. 2018

FRA

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Section: Effects Of the Solvent Systemmentioning

confidence: 99%

Total Phenolic, Flavonoid and Alkaloid Contents, Oxidative DNA Damage Protective and Antioxidant Properties of Methanol and Aqueous Extracts of Dissotis rotundifolia Whole Plant

Darkwah

Adinortey

et al. 2018

FRA

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“…Aqueous extract of Brassica juncea showed weak inhibition of alpha‐glucosidase with IC 50 of 541.71 ppm (Mun'im, Andriani, Mahmudah, & Mashita, ). Two percent epigallocatechin‐3‐gallate from Camellia sinensis inhibited the alpha‐glucosidase with IC 50 of 10.2 ± 2.02 μg/ml (Vinholes & Vizzotto, ).…”

Section: Methodsmentioning

confidence: 99%

Enzymes inhibitors from natural sources with antidiabetic activity: A review

Alam

Shafique

Amjad

et al. 2018

Phytotherapy Research

130

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Natural products have been extensively investigated for antidiabetic therapy. Many of the natural products have direct or indirect effect in diabetes pathways as enzyme inhibitors. The most involved mechanisms are inhibition of intestinal alphaglucosidase and alpha-amylase, lens aldose reductase, oxidative stress protection, inhibition of formation of advanced glycation end products, inhibition of aldose reductase, lowering plasma glucose levels, altering enzyme activity of hexokinases and glucose-6-phosphate, synthesizing and releasing of insulin, postprandial hyperglycemia inhibition, stimulation of GLUT-4, decreasing activity of G6P, lowering the level of skeletal hexokinases, etc. The following medicinal plants products or extracts showed promising effects as enzyme inhibitors:officinale. Some of the group of phytochemicals isolated with enzyme inhibition activities are Alkaloids, sesquiterpene and saponins, polysaccharides, flavonoids, dietary fibers, ferulic acid, tannins, limonene, and oleuropeoside. This review will provide very useful material to enhance the efficiency of rational antidiabetic drug design.

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“…The theoretical values were calculated considering additive contributions of the individual herbs. 29 A: additive effect: theoretical and experimental values differences lower than 5%. S: synergistic effect: experimental values are more than 5% lower for EC50 when compared with theoretical values.…”

Section: Leafmentioning

confidence: 99%

“…Infusions' combination effect was determined by the Vinholes formula. 29 The classification as additive, synergistic, or antagonistic was performed by comparison of obtained IC 50 values with theoretical IC 50 value according to the published literature.…”

Section: Combination Index (Ci) Analysismentioning

confidence: 99%

In vitro Antioxidant Properties and α-Glucosidase Inhibition of Combined Leaf Infusions from Psidium guajava L., Syzygium polyanthum L., and Annona muricata L.

Rahayu¹,

Heng²,

Timotius³

2019

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develop into permanent cell dysfunction. 10-12 Superoxide radicals in DM cause various damage through multiple pathways, such as Advanced Glycation End Products (AGEs), polyol pathways, hexosamine pathways and Kinase C proteins. This widespread impairment eventually leads to complications of microvascular disease. 13,14 According to Glamočlija and Jevrić-Čaušević, 15 there are five main classes of oral pharmacological agents to treat type 2 diabetes: sulfonylureas, megglitinides, metformin (a biguanide), thiazolidinediones, and α-glucosidase inhibitors. α-Glucosidase anchored in ABSTRACT Introduction: Guava (P. guajava), bay (S. polyanthum), and soursop (A. muricata) known as natural medicine. Limited report is available on their antioxidant and α-glucosidase inhibitory activities of leaf infusion. The aims of this research were to compare the antioxidant and α-glucosidase inhibitory activities of leaf infusion from guava, bay, and either as individual or combined infusions, and to analyze the chemical composition of the leaf infusion. Methods: Air dried leaf powder of guava, bay and soursop were infused separately with boiled aquadest. The infusions were analyzed for their antioxidant activity against DPPH. The α-glucosidase inhibitory assay was conducted against α-glucosidase from Saccharomyces cerevisiae. Then the infusions scanned with UV-Vis spectroscopy and analyzed with LC-MS. The synergism activities of the combined infusion were measured. Results: Antioxidant activities of leaf infusions of guava and bay showed a comparable result IC 50 12.53 ± 0.55 and 10.76 ± 0.20 µg GAE/mL, but the infusion of soursop showed lower (IC 50 19.77 ± 0.35 µg GAE/mL) than BHT as positive control (11.6 ± 0.31 µg GAE/mL). If soursop infusion was not added, then the mixture of the guava and bay infusion showed an antioxidative synergistic effect. The α-glucosidase inhibitory activities of the guava, bay and soursop infusion (0.083 ± 0.01; 0.025 ± 0.007; 0.533 ± 0.039 µg GAE/mL, respectively) were stronger than acarbose (1285 ± 148 µg/mL). The α-glucosidase inhibitory activities of the combined infusions showed a synergistic effect. The main constituents of the guava infusion were identified tentatively as chrysin and caffeoylquinic acid, for the bay infusion it was caffeoylquinic, and for the soursop infusion it was luteolin. Conclusions: There is a significant synergism of antioxidant activity of Guava and Bay mixture. The combined infusion of Bay and Soursop or Guava and Soursop showed antagonistic effect.

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Synergisms in alpha-glucosidase inhibition and antioxidant activity of Camellia sinensis L. Kuntze and Eugenia uniflora L. Ethanolic Extracts

Cited by 39 publications

References 42 publications

Total Phenolic, Flavonoid and Alkaloid Contents, Oxidative DNA Damage Protective and Antioxidant Properties of Methanol and Aqueous Extracts of Dissotis rotundifolia Whole Plant

Total Phenolic, Flavonoid and Alkaloid Contents, Oxidative DNA Damage Protective and Antioxidant Properties of Methanol and Aqueous Extracts of Dissotis rotundifolia Whole Plant

Enzymes inhibitors from natural sources with antidiabetic activity: A review

In vitro Antioxidant Properties and α-Glucosidase Inhibition of Combined Leaf Infusions from Psidium guajava L., Syzygium polyanthum L., and Annona muricata L.

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