Synergistic action between extracellular products from white-rot fungus and cellulase significantly improves enzymatic hydrolysis

Abstract

With a set of perfect extracellular lignocellulolytic enzymes, white-rot fungus has been recognized as playing an important role in the degradation of lignocellulose materials, which leads to the possibility of creating a composite enzymatic system with high hydrolysis efficiency in vitro. Echinodontium taxodii is a promising white-rot fungus for biologic pretreatment. In this study, we extracted the extracellular products of E. taxodii under solid-state fermentation conditions, mixed the extracellular products with cellulase to build a composite enzymatic system, and systematically evaluated the effect of this system on the hydrolysis of acid-pretreated and raw maize stovers. We found that the extracellular products from E. taxodii could significantly improve the hydrolysis efficiency of cellulase, with a synergistic action between the extracellular products and cellulase. Corn stovers treated with extracellular products were suitable for the enzymatic hydrolysis of cellulase. Furthermore, we found that pure proteins from the extracellular products were not sufficient to generate synergistic action. This finding suggests that non-protein substances may also be involved in the synergistic action between the extracellular products and cellulase.

Colonization of oil palm empty fruit bunches by basidiomycetes from the Brazilian cerrado: Enzyme production

“…Quantification of glucose and xylose, after enzymatic hydrolysis, was performed through high‐performance liquid chromatography (HPLC) (Agilent Technologies), under the following conditions: Aminex ® Column HPX‐87H (300 × 7.8 mm, Bio‐Rad, solution of H2SO4 (5 mM)) as the mobile phase; column temperature at 45°C, running time of 12 min and a flow rate of 0.6 ml min −1 . The hydrolysis yields were determined compared to the theoretical maximum 7 …”

“…That has encouraged exploratory studies of the enzyme pool produced during cultivation to evaluate which enzymes are present in the crude extract and how they interact with other enzymes. Thus, some initiatives have suggested mixing these crudes enzyme extracts in hydrolytic combinations or cocktails to investigate possible synergistic actions with cellulases and, consequently, savings in using high concentrations of commercial enzymes 7 …”

Sequential Action of Different Fiber-Degrading Enzymes Enhances the Degradation of Corn Stover

“…Additionally, it is used as a source of feedstock for the production of biofuel or as forage for ruminants [1][2][3][4]. Besides this, corn stover has a characteristic high carbohydrate content, ease of carbohydrate absorption as well as low lignification degree [5][6][7]. A large amount of corn stover is produced annually all the over the world [2,5,8].…”

“…Besides this, corn stover has a characteristic high carbohydrate content, ease of carbohydrate absorption as well as low lignification degree [5][6][7]. A large amount of corn stover is produced annually all the over the world [2,5,8]. However, almost 90% of this corn stover is burnt which causes severe environmental problems [2,9].…”

Improving the properties of straw biomass rattan by corn starch

“…At present, the straw leads to severe environmental pollution and resource waste [7]. Its processing methods include chemical, physical, and biological methods [8][9][10].…”