Synergistic action between fractions of essential oils from Cymbopogon citratus, Ocimum gratissimum and Thymus vulgaris against Penicillium expansum

Julienne, Nguefack; Tamgue, Ousman; Dongmo, J.B. Lekagne; Dakole, C.D.; Leth, Vibeke; Vismer, H. F.; Zollo, Paul Henri Amvam; Nkengfack, Augustin Ephrem

doi:10.1016/j.foodcont.2011.08.002

Cited by 123 publications

(70 citation statements)

References 18 publications

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“…It seems that the chemical type of EO ingredients plays a considerable role in the antimicrobial activity. Best results were achieved by EOs or EO compounds containing aromatic rings [10,12,15] and oxygenated terpenes [22]. Our MIC results also were lower for cinnamon, clove and thyme EOs containing aromatic compounds.…”

Section: Discussionmentioning

confidence: 53%

Effects of essential oil combinations on pathogenic yeasts and moulds

Horváth

Jenei

Vágvölgyi

et al. 2016

Acta Biologica Hungarica

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Essential oils (EOs) can be used as alternative or complementary antifungal agents against human pathogenic moulds and yeasts. To reduce the effective dose of antimicrobial agents, EOs are combined which can lead to synergistic or additive effect. In this study the anti-yeast and anti-mould activities of selected EOs were investigated, alone and in combinations, against clinical isolates of Candida albicans, C. parapsilosis, Aspergillus fumigatus, A. terreus, Rhizopus microsporus, Fusarium solani and Lichtheimia corymbifera. Minimum inhibitory concentrations (MICs) were determined for the EOs of cinnamon, citronella, clove, spearmint and thyme. To investigate the combination effect of the EOs, fractional inhibitory concentrations (FICs) were defined by the checkerboard method and the type of interaction was determined by the FIC index (FICI). FIC index below 0.5 was considered as synergism and between 0.5 and 1 as additive effect. Strongest antifungal activity was showed by thyme EO with MIC values below 1.0 mg/ml. Combination of EOs resulted in additive or indifferent effect, with occasional "borderline synergism". The best combination was cinnamon with clove leading to additive effect in all cases.

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Section: Discussionmentioning

confidence: 53%

Effects of essential oil combinations on pathogenic yeasts and moulds

Horváth

Jenei

Vágvölgyi

et al. 2016

Acta Biologica Hungarica

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“…Several investigations have shown that the essential oil of C. citratus possesses antibacterial (NAIK et al, 2010), antifungal (NGUEFACK et al, 2012), antioxidant (PEREIRA et al, 2009), anti-Leishmania (MACHADO et al 2012) and insecticidal effects (KUMAR et al, 2013). These properties are attributed primarily to the major constituent of the essential oil, the citral, which is a natural combination of two isomeric aldehydes (Figure 1), neral (cis-citral) and geranial (trans-citral).…”

Section: Introductionmentioning

confidence: 99%

Anthelmintic activity of Cymbopogon citratus against Haemonchus contortus

Macedo

Oliveira

Ribeiro

et al. 2015

Rev. Bras. Parasitol. Vet.

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Parasitic nematodes are of major economic importance in livestock. An alternative for the control of parasites is phytotherapy. This study evaluated the efficacy of Cymbopogon citratus decoction (CcD), C. citratus essential oil (CcEo) and citral against Haemonchus contortus using in vitro egg hatch test (EHT) and larval development test (LDT) and an in vivo test using a Meriones unguiculatus (gerbil) model. The effect of 800 mg/kg CcEo was evaluated in gerbils that had been artificially infected with 5,000 third-stage H. contortus larvae. The effective concentrations required to inhibit 50% (EC50) of egg hatching were 0.46, 0.14 and 0.13 mg/mL for CcD, CcEo and citral, respectively. The EC50 values in the LDT were 5.04, 1.92 and 1.37 mg/mL for CcD, CcEo and citral, respectively. H. contortus population in the group treated with C. citratus essential oil was reduced by 38.5% (P< 0.05) in comparison to the control group. These results suggest that it may be possible to use C. citratus essential oil to control of H. contortus parasite of small ruminant.Keywords: Phytotherapy, anthelmintic, Meriones unguiculatus, in vitro, citral. ResumoO parasitismo por nematoides tem grande importância econômica no rebanho. Uma alternativa para o controle de parasitas é a fitoterapia. Este estudo avaliou a eficácia do decocto de Cymbopogon citratus (DCc), do óleo essencial de C. citratus (OECc) e do citral contra Haemonchus contortus utilizando o teste in vitro de eclosão dos ovos (TEO) e o teste de desenvolvimento larval (TDL) e um teste in vivo com modelo Meriones unguiculatus (gerbil). O efeito de 800 mg/kg de OECc foi avaliado em gerbils infectados artificialmente com 5000 larvas de terceira fase de H. contortus. As concentrações efetivas necessárias para inibir 50% (CE50) da eclosão dos ovos foram 0,46; 0,14 e 0,13 mg/mL para DCc, OECc e citral, respectivamente. Os valores da CE50 no TDL foram de 5,04; 1,92 e 1,37 mg/mL para DCc, OECc e citral, respectivamente. No grupo tratado com óleo de C. citratus a população H. contortus foi reduzida em 38,5% (P < 0,05) em comparação com o grupo controle. Estes resultados sugerem que pode ser possível a utilização de óleo essencial de C. citratus para controle de H. contortus, parasita de pequenos ruminantes.Palavras-chave: Fitoterapia, anti-helmínticos, Meriones unguiculatus, in vitro, citral.

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“…There was a positive correlation between inhibition growth of A. flavus and the concentration of C. citrinus oil (p < 0.001; r = 0.873) and O. canum oil (p < 0.001; r = 0.768). Essentials oils from various sources exhibit broad-spectrum antimicrobial activity and their biological properties have been related to their chemical composition (Nguefack et al, 2012;Djenane et al, 2013). Indeed, compounds such as 1.8-cineole, pinene, limonene, terpineol and eugenol are present in both essential oils analyzed and have been shown to exert various biological activities including antifungal (Isman, 2000;Dayan et al, 2009).…”

Section: Effects Of Essential Oils On Fungal Growthmentioning

confidence: 99%

“…The hydrophobicity of the oils and their components allows them to partition in the lipid layer of the fungal cell membranes and result in disruption in membrane structure and cell membrane integrity (Beckman 2000). In addition, the inhibiting activities of these essential oils may not only be attributable to their major components but, to a synergistic effect of individual minor and/or major compounds (Nguefack et al, 2012;Sivakumar and Bautista-Banos, 2014).…”

Section: Effects Of Essential Oils On Fungal Growthmentioning

confidence: 99%