Synergistic actions of phytonutrient capped nanosilver as a novel broad-spectrum antimicrobial agent: unveiling the antibacterial effectiveness and bactericidal mechanism

Abstract

Nanosilver is a versatile biomedical agent that can potentially battle against bacterial infection. Herein, we introduce an energy-efficient green approach to synthesize phytonutrients modified silver nanoparticles (AgNPs) using cogon grass...

Evaluating Antioxidant Performance, Biosafety, and Antimicrobial Efficacy of Houttuynia cordata Extract and Microwave-Assisted Synthesis of Biogenic Silver Nano-Antibiotics

“…The presence of phytochemicals, such as phenolic compounds, flavonoids, alkaloids, glycosides, terpenoids, amino acid residues, and proteins, are considered to be the factors responsible for reducing silver ions by HC extract [47]. Generally, phenolic compounds are responsible for the reduction of Ag + ions to Ag atoms, which leads to the formation of AgNPs [24].…”

“…Moreover, the influence of silver nanomaterials on bacterial cells and the production of ROS in bacterial cells were also examined. The experimental finding revealed that HCE-AgNPs induce oxidative stress by damaging the cell wall, ultimately resulting in cell death [24,51]. Additionally, ATP, which is involved in the metabolism of cellular energy, was reduced due to the remarkable inhibition by the AgNPs, causing bacterial apoptosis.…”

“…The HCE-stabilized AgNPs were denoted as MW2 HCE-AgNPs, MW5 HCE-AgNPs, MW10 HCE-AgNPs, and MW20 HCE-AgNPs, respectively. Refluxmethod-mediated HCE-AgNPs were used as a control experiment, following the previously reported method [24]. After the completion of the reaction, the colloidal solutions were separated by centrifugation at 10,000× g for 20 min.…”

“…The hemolytic activity of MW HCE-AgNPs against human red blood cells (RBCs) was assessed with a modified version of a previously reported method [24]. Briefly, RBCs were first harvested based on the approved protocol (No.…”

Green Synthesis of Narrow-Size Silver Nanoparticles Using Ginkgo biloba Leaves: Condition Optimization, Characterization, and Antibacterial and Cytotoxic Activities

“…The generation of reactive oxygen species (ROS) is also considered one of the mechanisms underlying the antimicrobial activity of AgNPs. AgNPs and Ag + contribute to the production of hydroxyl radicals and ROS, thereby increasing the levels of cellular ROS and inhibiting bacterial cell growth [53]. The denaturation of DNA is also associated with ROS production, as oxidative damage to amino acids caused by oxygen radicals associated with AgNPs and Ag + ion exposure can disrupt the protein structure [54].…”