Synergistic activity and mode of action of flavonoids isolated from smaller galangal and amoxicillin combinations against amoxicillin-resistant Escherichia coli

Eumkeb, Griangsak; Siriwong, Supatcharee; Phitaktim, Sineewan; Rojtinnakorn, N.; Sakdarat, Santi

doi:10.1111/j.1365-2672.2011.05190.x

Cited by 75 publications

(54 citation statements)

References 35 publications

(34 reference statements)

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“…Kaempferide has been shown to lower the MIC of amoxicillin against amoxicillin resistant E. coli (AREC) when used in combination with this antibiotic [33]. These earlier findings together with our current results provide a strong support of the quorum quenching activity of kaempferide and its application in antimicrobial combination strategies.…”

Section: Screening For Qs Inhibition Using a Flavonoid Collectionsupporting

confidence: 72%

Congruence between graduating nursing students’ self-assessments and mentors’ assessments of students’ nurse competence

et al. 2016

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Quorum sensing (QS) is the process by which bacteria produce and detect signal molecules to coordinate their collective behavior. This intercellular communication is a relevant target for anti-biofilm therapies. Here we have optimized a screening-applicable assay to search for new quorum sensing inhibitors from natural compound libraries. In this system, QS is correlated with the production of violacein, which is directly controlled by the LuxI/LuxR system in Chromobacterium violaceum ATCC 31532. The parallel use of C. violaceum Tn5-mutant CV026, which depends on auto-inducer addition, allows simultaneous discrimination of compounds that act as quenchers of the AHL signal (quorum quenchers). The incorporation of a redox stain into the platform allowed further distinction between QS inhibitors, quorum quenchers and antibacterial compounds. A pilot screening was performed with 465 natural and synthetic flavonoids. All the most active compounds were flavones and they displayed potencies (IC 50 ) in the range of 3.69 to 23.35 µM. These leads were particularly promising as they inhibited the transition from microcolonies into mature biofilms from Escherichia coli and Pseudomonas aeruginosa strains. This approach can be very effective in identifying new antimicrobials posing lesser risks of resistance.

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Section: Screening For Qs Inhibition Using a Flavonoid Collectionsupporting

confidence: 72%

Congruence between graduating nursing students’ self-assessments and mentors’ assessments of students’ nurse competence

et al. 2016

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“…According to Osawa et al (1995), the enhanced growth responses indicate increased nutrient digestion and antioxidant effects of curcumin. Our results are in agreement with those of few other studies that revealed the stimulatory effects of turmeric on lipase activity, digestive enzyme activity and acid and alkaline protease activity, which all enhance the yellow pigmentation and growth rate of fish (Eumkeb et al, 2012;Kang et al, 2011). Crude protein is known to be significantly augmented while ether content decreases in fish response to turmeric supplementation (Cao et al, 2015).…”

Section: Discussionsupporting

confidence: 83%

Efficacy of Curcumin as an Immunostimulatory Dietary Supplement for Channel Catfish

Hafiz¹,

Srivastava²,

Newton³

et al. 2017

American Journal of Animal and Veterinary Sciences

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Natural agents such as curcumin may have the potential to enhance disease resistance in aquaculture. In this study, 30 catfish (Ictalurus punctatus), 2 years of age, were allotted to one of 3 treatment groups (n = 10) to receive diets supplemented with 0.0, 0.5, or 1.0% curcumin. Body weights and lengths of fish were taken at 0, 20, 40 and 60 days after starting the fish on experimental diets. At 60 days, blood samples were collected from 9 fish of each group and then were challenged with Aeromonas hydrophila (0.1 mL of 9×10 6 CFU/mL) intra-peritoneally. Morbidity and mortality was observed daily for 7 days following challenge. Serum separated from blood samples was analyzed for the cytokines, interleukin-4 (IL-4) and interleukin-12 (IL-12). Results indicated significantly higher body weights and lengths but lower levels of both IL-4 and IL-12 with curcumin at 0.5 and 1.0% than the control group. At 24 h following challenge with A. hydrophila, mortality was100 (9/9), 33 (3/9) and 22% (2/9) in the fish supplemented with 0.0, 0.5 and 1.0% curcumin, respectively. At 48 h following challenge, two more fish in the 0.5% group died but the fish remaining in 1.0% group survived even after 7 days. Results of this study suggest that curcumin has the potential to enhance performance of catfish and increase their disease resistance which may help in reducing use of antimicrobials in fish farming.

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“…The antibacterial activity of flavonoids can be exerted in three ways: directly kill the bacteria, synergistically activate the antibiotics, and attenuate the bacterial pathogenicity [16]. It is worth mentioning that the flavonoids have shown inhibitory activity against the efflux pump of MRSA [17], and also restrained the synthesis of peptidoglycan and ribosome in the cells of amoxicillin-resistant Escherichia coli (AREC) [18]. They have also shown inhibitory activity against different kinds of -lactamases produced by bacteria which are the key enzymes that disable the common antibiotics [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activities of Flavonoids: Structure-Activity Relationship and Mechanism

Fei¹,

Yang²,

Tang³

et al. 2014

CMC

600

422

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Flavonoids are well known as antibacterial agents against a wide range of pathogenic microorganism. With increasing prevalence of untreatable infections induced by antibiotic resistance bacteria, flavonoids have attracted much interest because of the potential to be substitutes for antibiotics. In this review, the structure-relationship of flavonoids as antibacterial agents is summarized, and the recent advancements on the antibacterial mechanisms of flavonoids are also discussed. It is concluded that hydroxyls at special sites on the aromatic rings of flavonoids improve the activity. However, the methylation of the active hydroxyl groups generally decreases the activity. Besides, the lipopholicity of the ring A is vital for the activity of chalcones. The hydrophobic substituents such as prenyl groups, alkylamino chains, alkyl chains, and nitrogen or oxygen containing heterocyclic moieties usually enhance the activity for all the flavonoids. The proposed antibacterial mechanisms of flavonoids are as follows: inhibition of nucleic acid synthesis, inhibition of cytoplasmic membrane function, inhibition of energy metabolism, inhibition of the attachment and biofilm formation, inhibition of the porin on the cell membrane, alteration of the membrane permeability, and attenuation of the pathogenicity.

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Synergistic activity and mode of action of flavonoids isolated from smaller galangal and amoxicillin combinations against amoxicillin-resistant Escherichia coli

Cited by 75 publications

References 35 publications

Congruence between graduating nursing students’ self-assessments and mentors’ assessments of students’ nurse competence

Congruence between graduating nursing students’ self-assessments and mentors’ assessments of students’ nurse competence

Efficacy of Curcumin as an Immunostimulatory Dietary Supplement for Channel Catfish

Antibacterial Activities of Flavonoids: Structure-Activity Relationship and Mechanism

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