Synergistic activity of polynuclear aromatic hydrocarbon mixtures as aryl hydrocarbon (Ah) receptor agonists

Chaloupka, K.; Harper, N.; Krishnan, Venkatesh; Santostefano, Michael J.; Rodriguez, L.; Safe, Stephen

doi:10.1016/0009-2797(93)90005-j

Cited by 63 publications

(28 citation statements)

References 29 publications

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“…On the other hand, the antiestrogenic activity of DEPE was not examined in the Meek's study, although the DEPE was found to activate AhR significantly (18). AhR liganded with AhR agonist has been known to mediate the antiestrogenic responses (5,6,14,15). There has been no information on the antiestrogenic effect of DEPE in AhRexpressing human cells.…”

Section: )-Induced Luciferase Activity Without Decreasing Cell Viabilmentioning

confidence: 95%

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Antiestrogenic Activity of Extracts of Diesel Exhaust Particulate Matter in MCF-7 Human Breast Carcinoma Cells

Okamura

Kizu

Toriba

et al. 2002

Polycyclic Aromatic Compounds

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Diesel exhaust particulate matter (DEP) was collected from three commercial diesel engine vehicles and DEP extracts (DEPEs) were prepared from each. The estrogenic and antiestrogenic activities of the DEPE samples were examined using an estrogen response element (ERE)-driven luciferase reporter gene assay in MCF-7 human breast cancer cells. While DEPE samples did not exhibit significant estrogen agonist activity alone, they suppressed 17β-estradiol (E 2 )-induced luciferase activity without decreasing cell viability. Several polycyclic aromatic hydrocarbons (PAHs) acting as aryl hydrocarbon receptor (AhR) agonists were detected in the DEPEs and their

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Section: )-Induced Luciferase Activity Without Decreasing Cell Viabilmentioning

confidence: 95%

“…DEP contains a variety of PAHs (12). Certain PAHs have been shown to exert antiestrogenic activity in cell-based and rodent studies (13)(14)(15)(16)(17). DEP extracts (DEPEs) were examined for estrogenic and antiestrogenic activities by…”

Section: )-Induced Luciferase Activity Without Decreasing Cell Viabilmentioning

confidence: 99%

Antiestrogenic Activity of Extracts of Diesel Exhaust Particulate Matter in MCF-7 Human Breast Carcinoma Cells

Okamura

Kizu

Toriba

et al. 2002

Polycyclic Aromatic Compounds

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“…DRCs constitute a group of polyhalogenated aromatic hydrocarbons that have similarities from the structural, chemical and toxicological point of view (McDonald et al, 1994). These persistent and bioaccumulative contaminants are carcinogenic (Chaloupka et al, 1993;Cleemann et al, 2000), immunosuppressive , and induce a number of pathological effects related to their endocrine disrupting action in mammals, including reproductive impairment (Schecter et al, 2006). Despite their toxicity, studies on environmental levels of DRCs in Brazil are scarce (de Assunção et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

High accumulation of PCDD, PCDF, and PCB congeners in marine mammals from Brazil: A serious PCB problem

Dorneles

Sanz

Eppe

et al. 2013

Science of The Total Environment

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• Dioxin-like PCBs accounted for over 83% of the total TEQ for all cetaceans • Negative correlations were found between length and TEQ values in franciscanas.• PCB concentrations found are among the highest ever reported for cetaceans. a b s t r a c t a r t i c l e i n f o Blubber samples from three delphinid species (false killer whale, Guiana and rough-toothed dolphin), as well as liver samples from franciscana dolphins were analyzed for dioxins and related compounds (DRCs). Samples were collected from 35 cetaceans stranded or incidentally captured in a highly industrialized and urbanized area (Southeast and Southern Brazilian regions). Dioxin-like PCBs accounted for over 83% of the total TEQ for all cetaceans. Non-ortho coplanar PCBs, for franciscanas (82%), and mono-ortho PCBs (up to 80%), for delphinids, constituted the groups of highest contribution to total TEQ. Regarding franciscana dolphins, significant negative correlations were found between total length (TL) and three variables, ΣTEQ-DRCs, ΣTEQ-PCDF and ΣTEQ non-ortho PCB. An increasing efficiency of the detoxifying activity with the growth of the animal may be a plausible explanation for these findings. This hypothesis is reinforced by the significant negative correlation found between TL and PCB126/PCB169 concentration ratio. DRC concentrations (ng/g lipids) varied from 36 to 3006, for franciscana dolphins, as well as from 356 to 30,776, for delphinids. The sum of dioxin-like and indicator PCBs varied from 34,662 to 279,407 ng/g lipids, for Guiana dolphins from Rio de Janeiro state, which are among the highest PCB concentrations ever reported for cetaceans. The high concentrations found in our study raise concern not only on the conservation of Brazilian coastal cetaceans, but also on the possibility of human health problem due to consumption of fish from Brazilian estuaries.

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“…Overestimates have also been reported in the analysis of poultry samples (Schoffer et al 2011) and ash (Till et al 1997). The difference between the two methods may be due to compounds that can trigger the aryl hydrocarbon receptor (AhR) pathway, such as polycyclic aromatic hydrocarbons (Chaloupka et al 1993), polybrominated compounds (Baston and Denison 2011), indol-3-carbinol (13C), which is present in cruciferous plants and has been described as a moderate activity binder with the AhR receptor (Chen et al 1996), in addition to DL-PCBs which in this study work were not analysed by HRGC/HRMS. In this sense, it is necessary to include a method of extraction and separation of DL-PCBs from dioxins in the sample.…”

Section: Comparison Of Hrgc/hrms and H4iie-erod Resultsmentioning

confidence: 99%

Application of the EROD-H4IIE bioassay for the determination of dioxins in pork in comparison to high resolution gas chromatography coupled to high resolution mass spectrometry

et al. 2013

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RESUMENEl bioensayo EROD-H4IIE se utiliza en la detección de hidrocarburos halogenados planares, presentando ventajas sobre la química analítica, como velocidad, simplicidad, precisión, alta sensibilidad y bajo costo, siendo un valioso bioensayo para estudios de biomonitoreo. Se ha aplicado exitosamente como método de cribado para la detección de dioxinas y furanos (PCDD/Fs) en carne de pollos broiler. En el presente trabajo realizamos un estudio para detectar PCDD/Fs en carne de cerdo, aplicando el bioensayo y la cromatografía de gases acoplada a espectrometría de masas de alta resolución (HRGC/ HRMS). Se tomaron 59 muestras compuestas de lomo vetado de cerdo en la planta faenadora de 6 planteles de Chile, durante el beneficio de animales entre 2004 y 2007 y una muestra fue tomada en 2011. Los concentraciones promedio de PCDD/Fs obtenidas mediante HRGC/HRMS oscilaron entre 0,22 y 0,34 pg WHO-TEQ/g de grasa. El contenido máximo fue 0,71 pg/g de grasa. El mayor valor promedio de los congéneres fue 0,1 pg/g de grasa de 2,3,4,7,8-PeCDF (entre 0,03 y 0,28 pg/g de grasa). Por tanto, las muestras no excedieron los límites máximos permitidos por la legislación nacional e internacional. Comparando ambos métodos, de las 59 muestras el bioensayo sobrestimó 36 resultados, subestimó 19 y 4 fueron similares. 15 muestras excedieron 1 pg TCDD-EQ/g de tejido y 5 superaron los 2 pg TCDD-EQ/g de tejido. No se estableció una equivalencia entre EROD-H4IIE y HRGC/ HRMS para la detección de PCDD/Fs en carne de cerdo, no existiendo asociación significativa entre las variables (r = -0,142, P ≥ 0,2840).Palabras clave: dioxinas, EROD/H4IIE, HRGC/HRMS, carne de cerdo. SUMMARYThe EROD-H4IIE cell bioassay is a screening method for the detection of planar halogenated hydrocarbons (PHH), it has several advantages over analytical chemistry like speed, simplicity, accuracy, high sensitivity and low cost which makes it a valuable bioassay for biomonitoring studies. It has been successfully used as screening method for the detection of dioxins and furans (PCDD/Fs) in meat from broiler chicken. The aim of this study was to detect PCDD/Fs in pork, by using both the bioassay and the high resolution gas chromatography/high resolution mass spectrometry (HRGC/ HRMS). 59 composite samples of pork chuck loin were taken at a slaughtering plant of 6 Chilean production facilities between 2004 and 2007, with one sample being taken in 2011. Mean concentrations of PCDD/Fs obtained by HRGC/HRMS ranged between 0.22 and 0.34 pg WHO-TEQ/g fat. The maximum was 0.71 pg WHO-TEQ/g fat. The average value of the congeners was 0.1 pg WHO-TEQ fat-2,3,4,7,8-PeCDF (between 0.03 and 0.28 pg/g fat). Therefore, the samples did not exceed the maximum allowed by national and international legislation. When comparing the results obtained with the two methods, 36 out of 59 sample results were overestimated by the bioassay, while 19 were underestimated and 4 were similar. 15 samples exceeded 1 pg/g TCDD-EQ tissue and only 5 exceeded 2 pg TCDD-EQ/g tissue. It was not possible to est...

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Synergistic activity of polynuclear aromatic hydrocarbon mixtures as aryl hydrocarbon (Ah) receptor agonists

Cited by 63 publications

References 29 publications

Antiestrogenic Activity of Extracts of Diesel Exhaust Particulate Matter in MCF-7 Human Breast Carcinoma Cells

Antiestrogenic Activity of Extracts of Diesel Exhaust Particulate Matter in MCF-7 Human Breast Carcinoma Cells

High accumulation of PCDD, PCDF, and PCB congeners in marine mammals from Brazil: A serious PCB problem

Application of the EROD-H4IIE bioassay for the determination of dioxins in pork in comparison to high resolution gas chromatography coupled to high resolution mass spectrometry

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