Synergistic Activity of the N-Terminal Peptide of Human Lactoferrin and Fluconazole against
            <i>Candida</i>
            Species

Lupetti, Antonella; Paulusma-Annema, Akke; Welling, Mick M.; Dogterom-Ballering, Heleen E. C.; Brouwer, Carlo P.J.M.; Senesi, Sonia; Dissel, Jaap T. van; Nibbering, Peter H.

doi:10.1128/aac.47.1.262-267.2003

Cited by 89 publications

(61 citation statements)

References 30 publications

(129 reference statements)

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“…The peptide was highly active against fluconazole-resistant Candida albicans at non-candidacidal concentrations, acted synergistically with fluconazole against this yeast and a fluconazole-sensitive C. albicans strain, as well as against C. glabrata, C. krusei, C. parapsilosis and C. tropicalis. When these yeasts were exposed to hLf(1-11) for five minutes and then incubated with fluconazole, they were effectively killed, while no candidacidal activity was observed when they were incubated first with fluconazole and then exposed to the peptide: this shows that the candidacidal activity is initiated by the peptide, whereas fluconazole is only required during the effector phase [53].…”

Section: In Vitro Antibacterial and Anti-fungal Activity Of Lf(1-11)mentioning

confidence: 98%

Antimicrobial Activity of Lactoferrin-Related Peptides and Applications in Human and Veterinary Medicine

Bruni¹,

et al. 2016

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Antimicrobial peptides (AMPs) represent a vast array of molecules produced by virtually all living organisms as natural barriers against infection. Among AMP sources, an interesting class regards the food-derived bioactive agents. The whey protein lactoferrin (Lf) is an iron-binding glycoprotein that plays a significant role in the innate immune system, and is considered as an important host defense molecule. In search for novel antimicrobial agents, Lf offers a new source with potential pharmaceutical applications. The Lf-derived peptides Lf(1-11), lactoferricin (Lfcin) and lactoferrampin exhibit interesting and more potent antimicrobial actions than intact protein.Particularly, Lfcin has demonstrated strong antibacterial, anti-fungal and antiparasitic activity with promising applications both in human and veterinary diseases (from ocular infections to osteo-articular, gastrointestinal and dermatological diseases).

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Section: In Vitro Antibacterial and Anti-fungal Activity Of Lf(1-11)mentioning

confidence: 98%

Antimicrobial Activity of Lactoferrin-Related Peptides and Applications in Human and Veterinary Medicine

Bruni¹,

et al. 2016

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“…hLF1-11 has a broad antimicrobial spectrum in vitro against both bacteria and fungi (11,12,16). Furthermore, Nibbering et al demonstrated the efficacy of hLF1-11 in vivo, reducing the number of bacteria (MRSA) after systemic administration in the mouse thigh infection model (16).…”

mentioning

confidence: 99%

Comparable Efficacies of the Antimicrobial Peptide Human Lactoferrin 1-11 and Gentamicin in a Chronic Methicillin-Resistant Staphylococcus aureus Osteomyelitis Model

Faber

Stallmann

Lyaruu

et al. 2005

Antimicrob Agents Chemother

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The therapeutic efficacy of an antimicrobial peptide, human lactoferrin 1-11 (hLF1-11), was investigated in a model of chronic methicillin-resistant Staphylococcus aureus (MRSA) (gentamicin susceptible) osteomyelitis in rabbits. We incorporated 50 mg hLF1-11/g or 50 mg gentamicin/g cement powder into a calcium phosphate bone cement (Ca-P) and injected it into the debrided tibial cavity, creating a local drug delivery system. The efficacy of hLF1-11 and gentamicin was compared to that of a sham-treated control (plain bone cement) (n ‫؍‬ 6) and no treatment (infected only) (n ‫؍‬ 5). The results were evaluated by microbiology, radiology, and histology. MRSA was recovered from all tibias in both control groups (n ‫؍‬ 11). On the other hand, hLF1-11 and gentamicin significantly reduced the bacterial load. Furthermore, no growth of bacteria was detected in five out of eight and six out of eight specimens of the hLF1-11-and gentamicin-treated groups, respectively. These results were confirmed by a significant reduction of the histological disease severity score by hLF1-11 and gentamicin compared to both control groups. The hLF1-11-treated group also had a significantly lower radiological score compared to the gentamicin-treated group. This study demonstrates the efficacy of hLF1-11 incorporated into Ca-P bone cement as a possible therapeutic strategy for the treatment of osteomyelitis, showing efficacy comparable to that of gentamicin. Therefore, the results of this study warrant further preclinical investigations into the possibilities of using hLF1-11 for the treatment of osteomyelitis.

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“…It has also been documented that several peptides have synergistic activity against C albicans when combined with fluconazole due to inhibition of production and release of intracellular ATP [28][29][30] . Because CaIPF14030 plays a critical role in regulating intracellular ATP levels, the protein encoded by CaIPF14030 may be targeted by the inhibitors of intracellular ATP.…”

Section: Discussionmentioning

confidence: 99%

CaIPF14030 negatively modulates intracellular ATP levels during the development of azole resistance in Candida albicans

et al. 2011

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Aim: Widespread and repeated use of azoles, particularly fluconazole, has led to the rapid development of azole resistance in Candida albicans. We investigated the role of CaIPF14030 during the development of azole resistance in C albicans. Methods: The expression of CaIPF14030 was measured by quantitative RT-PCR, and CaIPF14030 was disrupted by the hisG-URA3-hisG (URA-blaster) method. The sensitivity of C albicans to azoles was examined using a spot assay, and the intracellular ATP concentrations were measured by a luminometer. Results: CaIPF14030 expression in C albicans was up-regulated by Ca 2+ in a calcineurin-dependent manner, and the protein was overexpressed during the stepwise acquisition of azole resistance. However, disruption or ectopic overexpression of CaIPF14030 did not affect the sensitivity of C albicans to azoles. Finally, we demonstrated that disruption of CaIPF14030 significantly increased intracellular ATP levels, and overexpression significantly decreased intracellular ATP levels in C albicans. Conclusion: CaIPF14030 may negatively modulate intracellular ATP levels during the development of azole resistance in C albicans.

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Synergistic Activity of the N-Terminal Peptide of Human Lactoferrin and Fluconazole against Candida Species

Cited by 89 publications

References 30 publications

Antimicrobial Activity of Lactoferrin-Related Peptides and Applications in Human and Veterinary Medicine

Antimicrobial Activity of Lactoferrin-Related Peptides and Applications in Human and Veterinary Medicine

Comparable Efficacies of the Antimicrobial Peptide Human Lactoferrin 1-11 and Gentamicin in a Chronic Methicillin-Resistant Staphylococcus aureus Osteomyelitis Model

CaIPF14030 negatively modulates intracellular ATP levels during the development of azole resistance in Candida albicans

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