Synergistic anti-inflammatory effects ofLaminaria japonicafucoidan andCistanche tubulosaextract

Abstract

The anti-inflammatory effects of fuciodan and Cistanche tubulosa (CT) extract were investigated in vitro macrophage culture system and in vivo carrageenan-induced air pouch inflammation model. CT extract inhibited nitric oxide production from activated RAW 264.7 macrophage cells, while fucoidan was inactive. In vivo air pouch inflammation model, carrageenan-induced vascular exudation and increased nitric oxide and prostaglandin E2 concentrations in the exudates were synergistically suppressed by co-administration of fucoidan or CT extract. Moreover, tissue inflammation was substantially attenuated by the combinational therapy. However, there was no synergistic effect against the inflammatory cell infiltration, although fucoidan and CT extract each markedly reduced the cell numbers. Therefore, it is suggested that fucoidan blocks infiltration of inflammatory cells, while CT extract inhibits activation of the cells, and that their combinational treatment could be a promising candidate for the relief of various types of inflammation.

Fucoidan Prevents the Progression of Osteoarthritis in Rats

“…Fucoidans show many biological benefits for cardiovascular health, gastrointestinal health, 34 immunomodulation, 18 anti-inflammatory processes, 19,20 and anticoagulation. 21 Fucoidan is a commonly known high-molecular weight polysaccharide (200-500 kDa).…”

“…The GPC system consisted of three Ultrahydrogel Column Linear connections (1000-500-250; Waters), 0.2 M KNO 3 eluant used to irrigate the columns at 0.7 mL/min, and a refractive index detector (Waters 410 PDA detector; Waters). Fucoidan structure was similar to the structure of dextran sulfate (5,(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20), 500 kDa) purchased from Sigma-Aldrich.…”

“…Recently, it was discovered that fucoidan modulates diverse biological processes, including immunomodulatory, 18 anti-inflammatory, 19,20 and anticoagulant 21 effects. The osteoarthritic process may best be viewed as a chronic wound in which constant stimulation of innate inflammatory pathways by tissue damage can lead to a chronic remodeling response by all joint tissues.…”

Low Molecular Weight Fucoidan against Renal Ischemia–Reperfusion Injury via Inhibition of the MAPK Signaling Pathway

“…In our study, LMWF also significantly decreased MPO activity in kidney after IRI. Moreover, treatment with fucoidin blocks infiltration of inflammatory cells [22], and inhibits leukocyte rolling and reduces leukocyte emigration in a model of sepsis [23]. Fucoidan also suppresses adipogenesis through the inhibition of major markers and inflammation-related cytokines in adipocytes [24].…”

The enhancing effect of fucoidan derived from Undaria pinnatifida on immunoglobulin production by mouse spleen lymphocytes

“…It is well known that fucoidan has various biological effects [9][10][11] such as anti-tumor, [12][13][14][15][16][17][18] anti-virus 19,20) , and anti-inflammatory effects. 21) Furthermore, Mekabu-derived fucoidan showed anti-tumor effect in vivo and its mechanism was mediated by IFN-γ activated NK cells. 22) However, few data have been reported in relation to the effect of Mekabu components on Ig production.…”