Synergistic Antibacterial Activity of Clavulanic Acid and Amoxicillin Against β-Lactamase-Producing Strains of
            <i>Haemophilus ducreyi</i>

Girouard, Yves; Maclean, Ian; Ronald, Allan R.; Albritton, William L.

doi:10.1128/aac.20.1.144

Cited by 15 publications

(7 citation statements)

References 15 publications

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“…In contrast, amoxicillin-clavulanic acid, spectinomycin, and trimethoprim were less active in vitro against all isolates. The susceptibilities of the U.S. isolates fell within the range of those in previous reports (1,7,10,12,16,24,27,30,31,33). We observed, however, an association between plasmid content and antimicrobial susceptibilities.…”

Section: Resultscontrasting

confidence: 30%

Correlation between in vitro antimicrobial susceptibilities and beta-lactamase plasmid contents of isolates of Haemophilus ducreyi from the United States

Motley

Sarafian

Knapp

et al. 1992

Antimicrob Agents Chemother

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We determined the susceptibilities of 94 strains of Haemophilus ducreyi isolated in various municipalities in the United States between 1982 and 1989 to the following antimicrobial agents: amoxicillin-clavulanic acid, ceftriaxone, erythromycin, azithromycin, ciprofloxacin, ofloxacin, trimethoprim, and spectinomycin. Ceftriaxone (MIC, .0.008 ,g/ml), azithromycin (MIC, <0.125 ,Lg/ml), erythromycin (MIC, .0.125 ,ug/ml), ciprofloxacin (MIC, <0.25 ,ug/ml), and ofloxacin (MIC, .0.25 ,ug/ml) were highly active against all isolates. Amoxicillin-clavulanic acid (MICs, 0.25 to 8.0 ,ug/ml), trimethoprim (MICs, 0.06 to 16.0 ,ug/ml), and spectinomycin (MICs, 2.0 to .32.0 jLg/ml) were less active against these isolates. Isolates possessing the 5.7-MDa 1-lactamase plasmid were less susceptible to erythromycin, trimethoprim, and spectinomycin than were isolates possessing the 3.2-MDa I5-lactamase plasmid. The susceptibilities of plasmidless isolates to erythromycin, trimethoprim, and spectinomycin were distributed bimodally; the median MIC for the more susceptible plasmidless isolates corresponded to that for isolates with the 3.2-MDa plasmid, and the median MIC for the less susceptible plasmidless isolates corresponded to that for isolates with the 5.7-MDa plasmid. Thus, plasmid profiles may be valuable markers for geographical variations in antimicrobial susceptibilities of H. ducreyi strains that may indicate the relative efficacy of regimens for the treatment of chancroid. Of the regimens recommended by the U.S. Public Health Service for the treatment of chancroid, our results support the use of erythromycin, ceftriaxone, and ciprofloxacin, and perhaps ofloxacin, but suggest that amoxicillinclavulanic acid and sulfamethoxazole-trimethoprim should be used with caution.

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Section: Resultscontrasting

confidence: 30%

Correlation between in vitro antimicrobial susceptibilities and beta-lactamase plasmid contents of isolates of Haemophilus ducreyi from the United States

Motley

Sarafian

Knapp

et al. 1992

Antimicrob Agents Chemother

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“…The evaluation of the synergistic or antagonistic effect of antimicrobials can improve the use of antibiotics. Previously, the synergistic activity of amoxicillin with clavulanic acid against β-lactamase-producing strains of Haemophilus ducreyi was determined by agar dilution susceptibility testing [6].…”

Section: Introductionmentioning

confidence: 99%

Potential of Herbal Drug and Antibiotic Combination Therapy: A New Approach to Treat Multidrug Resistant Bacteria

Bhardwaj¹,

BR²,

DK³

2016

Pharm Anal Acta

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“…[2][3][4] Clavulanic acid, produced by Streptomyces clavuligerus, is an inhibitor of β-lactamases, providing a protection effect on hydrolysis of amoxicillin by β-lactamases; it also provides synergistic antibacterial effect. 5 A series of combination ratios (2:1, 4:1, 7:1, or 8:1) are commercially available. Increasing antibiotics resistance leads to less clinical efficacy, 6,7 which needs optimization of the dose regimen in the clinical treatment of old and new antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Rapid and Simultaneous Quantitation of Amoxicillin and Clavulanic Acid in Human Plasma and Urine by Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry and Its Application to a Pharmacokinetic Study

Fan

Zhao

et al. 2016

ANAL. SCI.

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Rapid, accurate and sensitive ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) methods were developed and validated for the simultaneous quantitation of amoxicillin and clavulanic acid in human plasma and urine samples. Amoxicillin and clavulanic acid in both plasma and urine were extracted using a solid-phase extraction method. The compounds were separated on an Acquity UPLC HSS T3 column (2.1 × 100 mm, 1.8 μm). Ampicillin was used as the internal standard (IS) in plasma, while amoxicillin-d4 and sulbactam were used as ISs in urine. The lower limit of quantitation was 0.0500 and 0.0250 μg/mL for amoxicillin and clavulanic acid in plasma, and 0.0500 μg/mL for both analytes in urine. The established methods were validated in terms of selectivity, precision, accuracy, linearity, matrix effect, recovery, carryover, interaction, dilution integrity and stability, and successfully applied to a pharmacokinetic study of amoxicillin sodium and clavulanate potassium (10:1) injection in healthy volunteers.

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Synergistic Antibacterial Activity of Clavulanic Acid and Amoxicillin Against β-Lactamase-Producing Strains of Haemophilus ducreyi

Abstract: The susceptibility of 47 strains of Haemophilus ducreyi to amoxicillin, clavulanic acid, and the combination of both drugs was determined by agar dilution susceptibility testing. A synergistic antibacterial activity of the combination was found for β-lactamase-producing strains.

Cited by 15 publications

References 15 publications

Correlation between in vitro antimicrobial susceptibilities and beta-lactamase plasmid contents of isolates of Haemophilus ducreyi from the United States

Correlation between in vitro antimicrobial susceptibilities and beta-lactamase plasmid contents of isolates of Haemophilus ducreyi from the United States

Potential of Herbal Drug and Antibiotic Combination Therapy: A New Approach to Treat Multidrug Resistant Bacteria

Rapid and Simultaneous Quantitation of Amoxicillin and Clavulanic Acid in Human Plasma and Urine by Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry and Its Application to a Pharmacokinetic Study

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