Synergistic antibacterial activity of surfactant free Ag–GO nanocomposites

Ahmad, Muhammad; Aslam, Saad; Mustafa, Faiza; Arshad, Usman

doi:10.1038/s41598-020-80013-w

Cited by 36 publications

(17 citation statements)

References 38 publications

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“…In the present study, Ag NPs and Ag/RGO NCs exhibited a strong plasma absorption band at ~395 nm ( Figure 2 ). Our results were in agreement with those of other studies [ 42 , 43 ].…”

Section: Resultssupporting

confidence: 94%

A Novel Green Preparation of Ag/RGO Nanocomposites with Highly Effective Anticancer Performance

et al. 2021

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The efficacy of current cancer therapies is limited due to several factors, including drug resistance and non-specific toxic effects. Due to their tuneable properties, silver nanoparticles (Ag NPs) and graphene derivative-based nanomaterials are now providing new hope to treat cancer with minimum side effects. Here, we report a simple, inexpensive, and eco-friendly protocol for the preparation of silver-reduced graphene oxide nanocomposites (Ag/RGO NCs) using orange peel extract. This work was planned to curtail the use of toxic chemicals, and improve the anticancer performance and cytocompatibility of Ag/RGO NCs. Aqueous extract of orange peels is abundant in phytochemicals that act as reducing and stabilizing agents for the green synthesis of Ag NPs and Ag/RGO NCs from silver nitrate and graphene oxide (GO). Moreover, the flavonoid present in orange peel is a potent anticancer agent. Green-prepared Ag NPs and Ag/RGO NCs were characterized by UV-visible spectrophotometry, transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), and dynamic light scattering (DLS). The results of the anticancer study demonstrated that the killing potential of Ag/RGO NCs against human breast cancer (MCF7) and lung cancer (A549) cells was two-fold that of pure Ag NPs. Moreover, the cytocompatibility of Ag/RGO NCs in human normal breast epithelial (MCF10A) cells and normal lung fibroblasts (IMR90) was higher than that of pure Ag NPs. This mechanistic study indicated that Ag/RGO NCs induce toxicity in cancer cells through pro-oxidant reactive oxygen species generation and antioxidant glutathione depletion and provided a novel green synthesis of Ag/RGO NCs with highly effective anticancer performance and better cytocompatibility.

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“…In the present study, Ag NPs and Ag/RGO NCs exhibited a strong plasma absorption band at ~395 nm ( Figure 2 ). Our results were in agreement with those of other studies [ 42 , 43 ].…”

Section: Resultssupporting

confidence: 94%

A Novel Green Preparation of Ag/RGO Nanocomposites with Highly Effective Anticancer Performance

et al. 2021

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“…The results showed that the silver particles are strongly attached to the GO surface and that the Ag/GO composites could inhibit the growth of S. aureus . A one-step route to Ag/GO nanocomposite formation, excluding the need for surfactants and reductants, was reported and a high antibacterial activity of the as-prepared Ag/GO nanocomposite toward E coli was found [ 47 ]. Synthesis, characterization, and antibacterial activity of Ag NPs decorated GO nanocomposite, fabricated by coprecipitation with green reducing agent, were reported also [ 48 ].…”

Section: Antimicrobial Activity and Applications Of Graphene Nanomaterialsmentioning

confidence: 99%

Antibiofouling Activity of Graphene Materials and Graphene-Based Antimicrobial Coatings

et al. 2021

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Microbial adhesion and biofilm formation is a common, nondesirable phenomenon at any living or nonliving material surface in contact with microbial species. Despite the enormous efforts made so far, the protection of material surfaces against microbial adhesion and biofilm formation remains a significant challenge. Deposition of antimicrobial coatings is one approach to mitigate the problem. Examples of such are those based on heparin, cationic polymers, antimicrobial peptides, drug-delivering systems, and other coatings, each one with its advantages and shortcomings. The increasing microbial resistance to the conventional antimicrobial treatments leads to an increasing necessity for new antimicrobial agents, among which is a variety of carbon nanomaterials. The current review paper presents the last 5 years’ progress in the development of graphene antimicrobial materials and graphene-based antimicrobial coatings that are among the most studied. Brief information about the significance of the biofouling, as well as the general mode of development and composition of microbial biofilms, are included. Preparation, antibacterial activity, and bactericidal mechanisms of new graphene materials, deposition techniques, characterization, and parameters influencing the biological activity of graphene-based coatings are focused upon. It is expected that this review will raise some ideas for perfecting the composition, structure, antimicrobial activity, and deposition techniques of graphene materials and coatings in order to provide better antimicrobial protection of medical devices.

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“…Generally, Ag with a smaller size and spherical shape has a higher surface energy than triangular Ag. Higher surface energies are unstable, so Ag NPs easily agglomerate [ 40 ] and diffuse into bacteria at a slow rate [ 41 ], thus reducing their antibacterial effect when compared with Ag NPrsms. The above explanations are possible mechanisms for Ag NPrsms’ superior bactericidal performance.…”

Section: Resultsmentioning

confidence: 99%

Prismatic Silver Nanoparticles Decorated on Graphene Oxide Sheets for Superior Antibacterial Activity

et al. 2022

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Spherical silver nanoparticles (Ag NPs) and silver nanoprisms (Ag NPrsms) were synthesized and decorated on graphene oxide (GO) nanosheets. The Ag contents were 29% and 23% in the GO–Ag NPs and GO–Ag NPrsms, respectively. The Ag NPrsms exhibited stronger (111) crystal signal than Ag NPs. The GO–Ag NPrsms exhibited higher Ag (I) content (75.6%) than GO-Ag NPs (69.9%). Increasing the nanomaterial concentration from 25 to 100 µg mL−1 improved the bactericidal efficiency, and the antibacterial potency was in the order: GO–Ag NPrsms > GO–Ag NPs > Ag NPrsms > Ag NPs > GO. Gram-positive Staphylococcus aureus (S. aureus) was more vulnerable than Gram-negative Escherichia coli (E. coli) upon exposure to these nanomaterials. The GO–Ag NPrsms demonstrated a complete (100%) bactericidal effect against S. aureus at a concentration of 100 µg mL−1. The GO–Ag composites outperformed those of Ag or GO due to the synergistic effect of bacteriostatic Ag particles and GO affinity toward bacteria. The levels of reactive oxygen species produced in the bacteria–nanomaterial mixtures were highly correlated to the antibacterial efficacy values. The GO–Ag NPrsms are promising as bactericidal agents to suppress biofilm formation and inhibit bacterial infection.

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Synergistic antibacterial activity of surfactant free Ag–GO nanocomposites

Cited by 36 publications

References 38 publications

A Novel Green Preparation of Ag/RGO Nanocomposites with Highly Effective Anticancer Performance

A Novel Green Preparation of Ag/RGO Nanocomposites with Highly Effective Anticancer Performance

Antibiofouling Activity of Graphene Materials and Graphene-Based Antimicrobial Coatings

Prismatic Silver Nanoparticles Decorated on Graphene Oxide Sheets for Superior Antibacterial Activity

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