Synergistic antioxidant effects of natural compounds on H2O2-induced cytotoxicity of human monocytes

Abstract

Natural compounds are endowed with a broad spectrum of biological activities, including protection against Toxins. Most of them are known for their antioxidant and radical scavenging activities. However, the synergistic combination of these natural molecules is not well studied. Therefore, the present study aims first to investigate the effect of four potent natural molecules [rosmarinic acid (Ros-A), ellagic acid (Ella-A), curcumin (Cur), and syringic acid (Syr-A)] on H2O2 -induced cell cytotoxicity and oxidative stress on the human monocytes (THP-1) and then to evaluate their combined action effect. Optimal combinations of these molecules were predicted using an augmented mixture design approach. In the first, as preliminary antioxidant activities screening, two in vitro assays were adopted to assess the single radicals scavenging activity of these natural compounds, DPPH• and ABTS• + tests. Based on the results obtained, the multitude of optimal formulas proposed by the mixture design study led to choosing four potent compositions (comp) in addition to ellagic acid, proposed as the most efficient when applied alone. The different molecules and mixtures were used to assess their cytoprotective effect on THP-1 cells in the presence and absence of H2O2. The most potent Comp-4, as well as the molecules forming this mixture, were exploited in a second experiment, aiming to understand the effect on oxidative stress via antioxidant enzyme activities analysis in the H2O2-induced oxidative stress in the THP-1 cell line. Interestingly, the natural molecules used for THP-1 cells treatment exhibited a significant increase in the antioxidant defense and glyoxalase system as well as suppression of ROS generation evaluated as MDA content. These results indicate that the natural compounds tested here, especially the synergistic effect of Cur and Ros-A (Comp-4), could serve as cytoprotective and immunostimulant agents against H2O2-induced cytotoxicity THP-1 cells, which makes them interesting for further investigations on the molecular mechanisms in preclinical animal models.

Innovative Delivery and Release Systems for Antioxidants and Other Active Substances in the Treatment of Cancer

“…However, the efficiency of these compounds is usually studied individually, and their synergistic effects are lacking. Several studies have addressed the intracellular generation of ROS by human monocytes (TPH-1) cells [ 29 ], a well-known model for studying the effects of toxins and/or the development of new scavengers. Ben Mrid et al [ 29 ] employed TPH-1 cells as a model to evaluate the cytoprotective effect of four natural compounds and their mixtures on H 2 O 2 -induced oxidative stress, analyzing their potential molecular mechanisms to avoid H 2 O 2 -oxidative damage.…”

“…Several studies have addressed the intracellular generation of ROS by human monocytes (TPH-1) cells [ 29 ], a well-known model for studying the effects of toxins and/or the development of new scavengers. Ben Mrid et al [ 29 ] employed TPH-1 cells as a model to evaluate the cytoprotective effect of four natural compounds and their mixtures on H 2 O 2 -induced oxidative stress, analyzing their potential molecular mechanisms to avoid H 2 O 2 -oxidative damage. Results showed that the addition of natural compounds, especially the synergistic effect of curcumin and rosmarinic acid, could act as cytoprotective and immunostimulant agents against H 2 O 2 -induced.…”

Combination Effect of Extracts and Pure Compounds of Endophytic Fungi Isolated from Sungkai (Peronema canescens) Leaves on Antioxidant Activity

“…Plant extracts and essential oils are among the many natural products that are used extensively across the world because of their antioxidant activity, which protects people against a variety of ailments brought on by oxidative stress (Aggrey et al, 2019;Sonam and Guleria, 2017;Srisuksomwong et al, 2023;Zulkefli et al, 2023). Synergistic interactions between various antioxidant chemicals in mixtures of synthetic and natural antioxidants, natural products, or mixtures of various herbal extracts or plant essential oils can increase antioxidant capacity (Basavegowda and Baek, 2021;Ben Mrid et al, 2022;Sharma et al, 2020). In this study, the same principle was applied to combinations of the extracts/ pure compounds from endophytic fungi.…”