Synergistic biodegradation of pentachlorophenol by Bacillus cereus (DQ002384), Serratia marcescens (AY927692) and Serratia marcescens (DQ002385)

Abstract

The consortium of Bacillus cereus (DQ002384), Serratia marcescens (AY927692) and Serratia marcescens (DQ002385) were used for pentachlorophenol (PCP) degradation. The consortia showed better overall removal efficiencies than single strains by utilization of PCP as a carbon and energy source confirmed by pH dependent dye indicator bromocresol purple (BCP) in mineral salt media (MSM). Mixed culture was found to degrade up to 93% of PCP (300 mg/l) as compared to single strains (62.75-90.33%), at optimized conditions (30 ± 1°C, pH 7 ± 0.2, 120 rpm) at 168 h incubation. PCP degradation was also recorded at 20°C (62.75%) and 37°C (83.33%); pH 6 (70%) and pH 9 (75.16%); 50 rpm (73.33%) and 200 rpm (91.63%). The simultaneous release of chloride ion up to 90.8 mg/l emphasized the bacterial dechlorination in the medium. GC-MS analysis revealed the formation of low molecular weight compound, i.e., 6-chlorohydroxyquinol, 2,3,4,6-tetrachlorophenol and tetrachlorohydroquinone, from degraded sample as compared to control.

Isolation and process parameter optimization of Brevibacterium casei for simultaneous bioremediation of hexavalent chromium and pentachlorophenol

“…The oxygen level of the growth medium strongly affects enzymatic processes involved in bacterial metabolism . Our results suggested that concomitant chromate reduction and PCP degradation was more pronounced in shaking condition with maximum detoxification observed at 120 rpm.…”

“…They found that addition of glucose could not enhance PCP biodegradation rate of Pseudomonas mendocina NSYSU. In contrary to it other researchers have reported that the bacterial strains were able to grow and degrade PCP only when glucose was added as a co‐substrate in the medium .…”

“…Our results are in accordance with the findings of Singh et al . who have also observed a gradual increase in PCP degradation with increasing aeration level. The rate of simultaneous detoxification was also dependent on initial Cr 6+ and PCP concentrations.…”

“…The ring cleavage activity of TVS‐3 strain was performed to determine that the fission of chloride ion of PCP is either from ortho or meta position . Ten ml of culture was centrifuged at 10,000 rpm for 15 min to remove the bacterial biomass.…”

Isolation, identification and characterization of a glyphosate-degrading bacterium, Bacillus cereus CB4, from soil

“…According to sequence analysis of 16S rRNA and the Biolog method, the strain was identifi ed as B. cereus. It was reported previously that B. cereus could degrade 2-aminoethylphosphonate (La Nauze and Rosenberga, 1968), chlorpyrifos (Lakshmi et al, 2009), pentachlorophenol (Singh et al, 2009), benzene (Dou et al, 2010), etc. To our knowledge, this is the fi rst report on degradation of glyphosate, the broad-spectrum herbicide, by B. cereus.…”