Synergistic effect of atorvastatin and Cyanidin-3-glucoside on angiotensin II-induced inflammation in vascular smooth muscle cells

Pantan, Rungusa; Tocharus, Jiraporn; Suksamrarn, Apichart; Tocharus, Chainarong

doi:10.1016/j.yexcr.2016.02.017

Cited by 32 publications

(23 citation statements)

References 47 publications

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“…In addition to a lipid-lowering effect, statins appear to protect against pathomechanisms associated with the NRF2 network such as inflammatory (Pantan et al, 2016;Wu et al, 2016a;Hwang et al, 2017) and pathologic ROS formation (Abdanipour et al, 2014). They are competitive inhibitors of 3-hydroxy-3-methyl-glutaryl-CoA reductase, which catalyzes the rate-limiting reaction in cholesterol synthesis.…”

Section: E Repurposing Instead Of De Novo Drug Discovery and Developmentioning

confidence: 99%

Transcription Factor NRF2 as a Therapeutic Target for Chronic Diseases: A Systems Medicine Approach

et al. 2018

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Section: E Repurposing Instead Of De Novo Drug Discovery and Developmentioning

confidence: 99%

Transcription Factor NRF2 as a Therapeutic Target for Chronic Diseases: A Systems Medicine Approach

et al. 2018

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“…[1][2][3] It is the most common member of the anthocyanin family. 4 Many studies describe C3G as an antioxidant [4][5][6][7] contributing to anti-inflammation, [7][8][9] anti-obesity, 10,11 and anti-diabetic 7,[11][12][13] processes, and to the reduction of risk of cardiovascular diseases. 7,14 Furthermore, several reports show the C3G's inhibitory effect on cancer metastasis, tumor progression and cell migration in lung, 15,16 and breast 17,18 cancer cell lines.…”

Section: Introductionmentioning

confidence: 99%

Cyanidin‐3‐glucoside binds to talin and modulates colon cancer cell adhesions and 3D growth

Baster

Kukkurainen

et al. 2020

FASEB j.

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Cyanidin-3-glucoside (C3G) is a natural pigment, found in many colorful fruits and vegetables. It has many health benefits, including anti-inflammation, cancer prevention, and anti-diabetes. Although C3G is assumed to be an antioxidant, it has been reported to affect cell-matrix adhesions. However, the underlying molecular mechanism is unknown. Here, we show that the expression of talin1, a key regulator of integrins and cell adhesions, negatively correlated with the survival rate of colon cancer patients and that depletion of talin1 inhibited 3D spheroid growth in colon cancer cells. Interestingly, C3G bound to talin and promoted the interaction of talin with β1A-integrin. Molecular docking analysis shows that C3G binds to the interface of the talin-β-integrin complex, acting as an allosteric regulator and altering the interaction between talin and integrin. Moreover, C3G promoted colon cancer cell attachment to fibronectin. While C3G had no significant effect on colon cancer cell proliferation, it significantly inhibited 3D spheroid growth in fibrin gel assays. Since C3G has no or very low toxicity, it could be potentially used for colon cancer prevention or therapy. K E Y W O R D Sadhesion, cancer prevention, cyanidin-3-glucoside, drug discovery, molecular docking, talin1, talin2, β-integrin 2228 | BASTER ET Al. | 2229BASTER ET Al. | Cell proliferation assaysCells were trypsinized and resuspended in DMEM containing 10% FBS at a density of 5 × 10 4 cells/mL. Cells were treated with C3G or vehicle (DMSO) where was indicated. One hundred microliters of cells was added to each well of 96-well tissue culture plates. The cells were allowed to grow for 1-4 days (as indicated) in a CO2 incubator and then incubated with 10 µL (per well) of TetraZ (WST-8) cell counting kit. Cell proliferation was determined by measuring absorbance 450 nm using a BioTek plate reader.

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“…Many studies have shown that RSV has pleiotropic anti‐inflammatory and antioxidant effects and that it also improves endothelial function (Deng et al, ; Nade et al, ; Schäfer et al, ; Suh et al, ). However, other studies have reported that statins can promote fat deposition in the liver and that there are reverse effects after withdrawal from high doses of statins (Pantan et al, ). Therefore, interventions of statins and natural products have gained interest to avoid side effects and to improve the effect of statins.…”

Section: Discussionmentioning

confidence: 98%

“…RSV has been shown to have pleiotropic anti-inflammatory and antioxidant properties; in addition, it can improve endothelial function (Deng et al, 2015;Nade, Kawale, & Patel, 2015;Schäfer et al, 2007;Suh et al, 2010). In contrast, a previous study reported adverse effects of high doses of statin in the form of reverse effects after withdrawal (Pantan, Tocharus, Suksamrarn, & Tocharus, 2016). Therefore, interventions of statin and natural products have gained interest to avoid side effects and to improve the effect of statin.…”

Section: Rosuvastatin (Rsv; 3-hydroxy-3-methylglutaryl Coenzyme-a [Hmg-mentioning

confidence: 99%

Synergistic effects of the capsaicinoid nonivamide and rosuvastatin on obesity‐related endothelial dysfunction in rat fed a high‐fat diet

Sivasinprasasn

Wikan

Tocharus

et al. 2019

Phytotherapy Research

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Capsaicinoid nonivamide (PAVA) and rosuvastatin (RSV) have been shown to exert antioxidant and anti‐obesity effects in various animal models, but it is unknown whether their combination would be an effective treatment for obesity‐related endothelial dysfunction. This study aimed to investigate the mechanism of PAVA in synergy with RSV. Male Sprague–Dawley rats were given a high‐fat diet (HFD) or normal diet during a 20‐week period. At 16 weeks, rats in each diet group were divided into subgroups. Normal diet rats were divided into Normal diet control, Normal diet with PAVA, and Normal diet with RSV groups. HFD rats were subdivided into HFD control, HFD with PAVA, HFD with RSV, and HFD with PAVA + RSV groups and evaluated for metabolic parameters, blood pressure, aortic function, and histological change of the aorta in rats. Our results showed the combined therapy had a significantly greater effect than the monotherapy in all measured parameters; this was indicated by improvement in insulin sensitivity and aortic function, decreased blood pressure, lower oxidative stress, and prevention of vascular damage. The synergistic effect of the PAVA and RSV can protect HFD‐induced obesity‐related endothelial dysfunction, suggesting that the combination of PAVA and RSV could be an effective alternative treatment for obesity‐related complications in patients with cardiovascular disease.

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Synergistic effect of atorvastatin and Cyanidin-3-glucoside on angiotensin II-induced inflammation in vascular smooth muscle cells

Cited by 32 publications

References 47 publications

Transcription Factor NRF2 as a Therapeutic Target for Chronic Diseases: A Systems Medicine Approach

Transcription Factor NRF2 as a Therapeutic Target for Chronic Diseases: A Systems Medicine Approach

Cyanidin‐3‐glucoside binds to talin and modulates colon cancer cell adhesions and 3D growth

Synergistic effects of the capsaicinoid nonivamide and rosuvastatin on obesity‐related endothelial dysfunction in rat fed a high‐fat diet

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