Synergistic effect of benzylaminopurine and meta-Topolin combination for micropropagation of gerbera ‘Pink Melody’

Shaheen, Aaqib; Dewir, Yaser Hassan; Kher, Mafatlal M.; Khan, Majid; El-Banna, Antar; Alaizari, Ahmed Ali

doi:10.1590/1413-7054202246017521

Cited by 4 publications

(1 citation statement)

References 35 publications

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“…One way to propagate endangered native plants is tissue culture. This technique allows a high multiplication rate (Oseni;Pande;Nailwal, 2018) and, if necessary, the maintenance of the species' genetic characteristics through micropropagation (Aaqib et al, 2022;Rodrigues et al, 2020). However, in vitro multiplication is still difficult for many native Cerrado species such as C. rufa.…”

Section: Introductionmentioning

confidence: 99%

Isolation, molecular identification, and in vitro control of Campomanesia rufa (O. Berg) Nied. endogenous bacteria

Mosqueira,

Paiva,

Rivas

et al. 2023

Ciênc. agrotec.

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Campomanesia rufa (O. Berg) Nied. is a Myrtaceae species native to the Brazilian Cerrado. As C. rufa is considered endangered, in vitro propagation is an alternative for its conservation. However, the lack of effective disinfection protocols for endophytic microorganisms naturally present in plant tissues hinders the success of micropropagation. In this context, the objective of this study was to isolate, molecular identify, and control endogenous occurring bacteria of C. rufa propagated in vitro. Purified PCR products of bacterial isolates were sequenced by the Sanger method and aligned with homologous sequences using the Basic Local Alignment Search Tool (BLAST) available in National Centre for Biotechnology Information (NCBI) database. MEGA7 software was used to align all sequences and to draw phylogenetic trees. Survival, shoot height, and efficiency of antibiotics (streptomycin, ampicillin, and chloramphenicol) at different concentrations in the culture medium were evaluated. Different Bacillus sp. strains were observed in the plant tissues. When testing the control of Bacillus sp. with antibiotics, 32 mg L-1 ampicillin caused a significant reduction in bacterial contamination with no effect on explant survival. On the other hand, 256 mg L-1 streptomycin caused the greatest reduction in contamination but was lethal to over 90% of the explants. This study is the first report on the occurrence of endogenous bacteria and their control in the context of in vitro native species conservation.

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Section: Introductionmentioning

confidence: 99%

Isolation, molecular identification, and in vitro control of Campomanesia rufa (O. Berg) Nied. endogenous bacteria

Mosqueira,

Paiva,

Rivas

et al. 2023

Ciênc. agrotec.

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Meta-Topolin induced highly efficient plant regeneration from various explants of eggplant (Solanum melongena L.)

Gande,

Marapaka,

Jogam

et al. 2024

Plant Cell Tiss Organ Cult

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Meta-Topolin induced highly efficient plant regeneration from various explants of eggplant (Solanum melongena L.)

Gande,

Marapaka,

Jogam

et al. 2024

Preprint

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Eggplant (Solanum melongena L.) is one of the essential vegetables worldwide, and cultivated genotypes of eggplant suffer from numerous abiotic and biotic stresses. A reproducible and efficient plant regeneration system is crucial for applying molecular breeding methods to overcome the difficulties of conventional breeding programs to improve eggplant germplasm, such as genetic transformation and genome editing techniques. The three explant types, cotyledon, hypocotyl, and leaf, were obtained from two different cultivars, Pusa purple long (PPL) and Pusa green red (PGR) of eggplant. Three explants were cultivated on media augmented with a variety of cytokinins like BAP, mT, and ZEA with different concentrations. The media supplemented with mT at 8.28 µM generated the highest number of shoots, which showed the optimum regeneration efficiency for all three explants in two eggplant genotypes. The cotyledon explants generated the optimum number of shoot buds on the medium amended with low concentrations of BAP (2.22 µM), KIN (2.32 µM), and ZEA (2.28 µM), and mT at 8.28 µM. The mT (8.28 µM) and BAP at 2.22 µM combinations produced 25.8 and 18.3 shoots in PPL and PGR genotypes, respectively. The addition of various concentrations of IAA (1.43 to 5.71 µM), IBA (1.23 to 4.92 µM), and NAA (1.34 to 5.37 µM) in combination with mT (8.28 µM) were evaluated to find out their role on the induction and proliferation of numerous shoot buds from cotyledon explants of two cultivars of eggplant. The medium augmented with mT (8.28 µM) and IAA (2.85 µM) produced 26.4 shoots and 17.8 shoots in cotyledon explants of PPL and PGR cultivars, respectively. The optimum rooting efficiency of shoots was recorded on the medium containing the IAA (5.71 µM) and produced complete plantlets. The plantlets showed 100% similarity with their mother plants.

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Synergistic effect of benzylaminopurine and meta-Topolin combination for micropropagation of gerbera ‘Pink Melody’

Cited by 4 publications

References 35 publications

Isolation, molecular identification, and in vitro control of Campomanesia rufa (O. Berg) Nied. endogenous bacteria

Isolation, molecular identification, and in vitro control of Campomanesia rufa (O. Berg) Nied. endogenous bacteria

Meta-Topolin induced highly efficient plant regeneration from various explants of eggplant (Solanum melongena L.)

Meta-Topolin induced highly efficient plant regeneration from various explants of eggplant (Solanum melongena L.)

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