Synergistic Effect of Charged Amino Acids on the Stability and Fibril Formation of Lysozyme
Subhasree Ghosh,
N. Prakash Prabhu
Abstract:Osmolytes alter the stability and aggregation of proteins. The effect of mixture of osmolytes is generally not a simple additive of their individual effects. Here, we examine the effect of different mixtures of charged amino acids (AAs) on the stability and fibrillation of lysozyme. Arg, Asp and Glu induce three‐state unfolding. The addition of Lys, Asp and Glu increases the thermal stability of lysozyme, whereas Arg does not alter the stability. The mixture of AAs destabilizes the intermediate, but the overal… Show more
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