Synergistic effect of cysteamine, leukemia inhibitory factor, and Y27632 on goat oocyte maturation and embryo development in vitro

An, Liyou; Liu, Jiao; Du, Yinyan; Liu, Zhihui; Zhang, Fenli; Liu, Yanhong; Ling, Pingping; Chang, Shiwei; Hu, Yeshu; Li, Yang; Xu, Bowen; Yang, Lan; Presicce, Giorgio Antonio; Du, Fuliang

doi:10.1016/j.theriogenology.2017.11.028

Cited by 16 publications

(18 citation statements)

References 41 publications

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“…Few studies have investigated the role of ROCK signaling in the maturation medium of oocytes in some animal species. Our findings identified ROCK as a key player in dromedary camel oocyte meiotic maturation as in the pigs [24,40], cattle [41], cats [29], caprinae [30,42], and mice [31,43]. Oocyte maturation is a crucial process through which oocytes acquire their intrinsic capacity to support fertilization and early embryo development.…”

Section: Discussionmentioning

confidence: 74%

“…The action and interaction of various related proteins or their combined effect could be speciesdependent. For example, in goat oocytes, RI decreased the maturation rate from 45% (Control) to 42%, but when there is special combination of RI with cysteamine and leukemia inhibitory factor, the maturation rate was improved to 67% [30], owing to the synergistic effects of RI and the prevention of apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…ROCK signaling is a regulatory network for prevention of mitochondrial injury and apoptosis [65,66]. Some studies have highlighted antiapoptotic effects of RI on oocytes that could rescue the damaging effects of vitrification [30,67]. The ratio of BAX/BCL2 reflects the balance between apoptotic and antiapoptotic signals and determines the cell's susceptibility to apoptosis and the fate of cell survival [68][69][70].…”

Section: Discussionmentioning

confidence: 99%

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Effects of Short-Term Inhibition of Rho Kinase on Dromedary Camel Oocyte In Vitro Maturation

Tukur

Aljumaah

Swelum

et al. 2020

Animals

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This is the first report on a biphasic in vitro maturation (IVM) approach with a meiotic inhibitor to improve dromedary camel IVM. Spontaneous meiotic resumption poses a major setback for in vitro matured oocytes. The overall objective of this study was to improve in vitro maturation of dromedary camel oocytes using ROCK inhibitor (Y-27632) in a biphasic IVM to prevent spontaneous meiotic resumption. In the first experiment, we cultured immature cumulus–oocyte complexes (COCs, n = 375) in a prematuration medium supplemented with ROCK inhibitor (RI) for 2 h, 4 h, 6 h, and 24 h before submission to normal in vitro maturation to complete 28 h. The control was cultured for 28 h in the absence of RI. In the first phase of experiment two, we cultured COCs (n = 480) in the presence or absence (control) of RI for 2 h, 4 h, 6 h, and 24 h, and conducted real-time relative quantitative PCR (qPCR) on selected mRNA transcripts. The same was done in the second phase, but qPCR was done after completion of normal IVM. Assessment of nuclear maturation showed that pre-IVM for 4 h yielded an increase in MII oocyte (54.67% vs. 26.6% of control; p < 0.05). As expected, the same group showed the highest degree (2) of cumulus expansion. In experiment 2, qPCR results showed significantly higher expression of ACTB and BCL2 in the RI group treated for 4 h when compared with the other groups. However, their relative quantification after biphasic IVM did not reveal any significant difference, except for the positive response of BCL2 and BAX/BCL2 ratio after 4 and 6 h biphasic IVM. In conclusion, RI prevents premature oocyte maturation and gave a significantly positive outcome during the 4 h treatment. This finding is a paradigm for future investigation on dromedary camel biphasic IVM and for improving the outcome of IVM in this species.

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Short-Term Inhibition of Rho Kinase on Dromedary Camel Oocyte In Vitro Maturation

Tukur

Aljumaah

Swelum

et al. 2020

Animals

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“…The receptor complex mediates downstream LIF signaling via multiple pathways, including JAK/STAT (Janus Kinase/Signal Transducer and Activator of Transcription), MAPK (Mitogen-Activated Protein Kinases) and PI3K/AKT (phosphatidylinositol 3-kinase/AKT serine/threonin kinase) [ 17 ]. LIF appears to play key roles during follicular growth and improves in vitro cumulus expansion and oocyte competence, embryo development [ 18 , 19 , 20 , 21 , 22 ], and cellular cryotolerance to vitrification (Kocyigit and Cevik 2015) in different species.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Maturation with Leukemia Inhibitory Factor Prior to the Vitrification of Bovine Oocytes Improves Their Embryo Developmental Potential and Gene Expression in Oocytes and Embryos

Vendrell-Flotats

García-Martínez

Martínez-Rodero

et al. 2020

IJMS

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Oocyte cryopreservation has a significant impact on subsequent embryonic development. Herein, we investigated whether supplementing in vitro maturation medium with Leukemia Inhibitory Factor (LIF) prior to vitrification affects embryo development and gene expression at different embryo developmental stages. A panel of genes including maternal effect, epigenetics, apoptosis and heat stress was relatively quantified. The results show reduced cleavage rates after vitrification, regardless of the LIF treatment. Although not statistically different from control-vitrified oocytes, oocyte apoptosis and the blastocyst yield of LIF-vitrified oocytes were similar to their non-vitrified counterparts. Vitrification increased oocyte ZAR1, NPM2 and DPPA3 gene expression while its expression decreased in LIF-vitrified oocytes to similar or close levels to those of non-vitrified oocytes. With a few gene-specific exceptions, vitrification significantly increased the expression of DNMT3A, HDAC1, KAT2A, BAX and BCL2L1 in oocytes and most stages of embryo development, while comparable expression patterns for these genes were observed between LIF-vitrified and non-vitrified groups. Vitrification increased HSPA1A expression in oocytes and HSP90AA1 in 2-cell embryos. Our data suggest that vitrification triggers stage-specific changes in gene expression throughout embryonic development. However, the inclusion of LIF in the IVM medium prior to vitrification stimulates blastocyst development and several other developmental parameters and induces oocytes and embryos to demonstrate gene expression patterns similar to those derived from non-vitrified oocytes.

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“…It plays a crucial role during follicular development and ovulation, promoting primordial to primary follicle transition in rats 29 and to coordinate follicular growth and ovulation in mice 30 . LIF has also been reported to promote oocyte maturation in mice 31 , cattle 32 , pigs 33 and goats 34 when added to maturation medium. In addition, murine and bovine oocytes matured with LIF lead to higher blastocyst rates 31,32 .…”

mentioning

confidence: 99%

In vitro maturation in the presence of Leukemia Inhibitory Factor modulates gene and miRNA expression in bovine oocytes and embryos

Vendrell-Flotats

García-Martínez

Martínez-Rodero

et al. 2020

Sci Rep

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Members of the interleukin-6 (IL-6) family of cytokines are important for reproductive function that are mediated through changes in gene and miRNA expression. Herein, we characterized the expression of miR-21, miR-155, miR-34c and miR-146a in bovine oocytes and cumulus cells during in vitro maturation (IVM) with leukemia inhibitory factor (LIF), IL-6 and IL-11 or unsupplemented controls. LIF-exposed COCs showed higher expression of miR-21 and miR-155 in oocytes, whereas miR-146a expression was increased in oocytes matured with IL-6 and IL-11. In cumulus cells, miR-155 expression was elevated by all treatments while only LIF increased miR-21 expression. Based on these results, we next examined how LIF exposure during IVM affected oocyte competence, through IVF and the expression of specific genes in GV- and MII-oocytes, in 2- and 8-cell embryos, and in Day 8-blastocysts. LIF supplementation did not affect cleavage rate, blastocyst yield or several other developmental parameters, but did increase hatching rate. LIF suppressed DPPA3, ZAR1 and NPM2 expression in 2 cell- and/or 8-cell embryos. LIF increased the expression of KAT2A and HSPA1A in MII-oocytes, and that of HDAC1, KAT2A and HSP90AA1 and the BAX:BCL2L1 ratio in 2-cell embryos. In contrast, HDAC1, KAT2A and HSP90AA1 expression and BAX:BCL2L1 ratio was lower in 8-cell embryos derived from LIF oocytes. IVM with LIF also increased the expression of DNMT3A, HSPA1A and HSP90AA1 in blastocysts. In conclusion, supplementation with LIF during IVM was consistently associated with changes in the relative abundance of transcripts in mature bovine oocytes and in specific embryo developmental stages.

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Synergistic effect of cysteamine, leukemia inhibitory factor, and Y27632 on goat oocyte maturation and embryo development in vitro

Cited by 16 publications

References 41 publications

Effects of Short-Term Inhibition of Rho Kinase on Dromedary Camel Oocyte In Vitro Maturation

Effects of Short-Term Inhibition of Rho Kinase on Dromedary Camel Oocyte In Vitro Maturation

In Vitro Maturation with Leukemia Inhibitory Factor Prior to the Vitrification of Bovine Oocytes Improves Their Embryo Developmental Potential and Gene Expression in Oocytes and Embryos

In vitro maturation in the presence of Leukemia Inhibitory Factor modulates gene and miRNA expression in bovine oocytes and embryos

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