Synergistic effect of pretreatment and hydrolysis enzymes on the production of fermentable sugars from date palm lignocellulosic waste

Al‐Zuhair, Sulaiman; Ahmed, Khalda; Abdulrazak, Ashir; El‐Naas, Muftah H.

doi:10.1016/j.jiec.2012.09.022

Cited by 29 publications

(6 citation statements)

References 15 publications

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“…These improvements were explained to be based on the reduction of the unspecific adsorption of hydrolases on enzyme-treated lignin. Similar results were obtained by Al-Zuhair et al [134], which showed an increment of sugar production from 5.6% to 45.6% after treatment of a milled material from date palm lignocellulosic waste with T. versicolor laccase and the mediator HBT. Using the same LMS and the pyrolysis/gas chromatography-mass spectrometry (Py/GC-MS) with tetramethylammonium hydroxide (TMAH) thermochemolysis analysis, Chen et al [135] described a significant lignin modification (lignin side chain oxidation) after treatment of ensiled corn stover.…”

Section: Delignification By Laccase-mediator System (Lms)supporting

confidence: 89%

Biofuels and Biochemicals Production

Ezeji¹

2017

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Section: Delignification By Laccase-mediator System (Lms)supporting

confidence: 89%

Biofuels and Biochemicals Production

Ezeji¹

2017

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“…indicated that reduction of lignin content or ferulate-lignin crosslinking would improve the fermentation of cellulose and hemicellulose more than current approaches for shifting lignin composition [ 10 ]. In a recent study, a higher reducing sugars conversion (60%) was achieved when adding commercial laccase into a mixture of cellulases and hemicellulases at 45°C [ 11 ]. Therefore, ligninase should be taken into account in enzyme cocktail construction for saccharification in order to maximize the hydrolysis efficiency.…”

Section: Introductionmentioning

confidence: 99%

Synergy of crude enzyme cocktail from cold-adapted Cladosporium cladosporioides Ch2-2 with commercial xylanase achieving high sugars yield at low cost

Yang

Fan

et al. 2014

Biotechnol Biofuels

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BackgroundThe efficiency and cost of current lignocellulosic enzymes still limit the large-scale production of cellulosic ethanol in industry. Residual lignin after pretreatment severely depresses the activity of polysaccharide hydrolases and the h ydrolysis of holocellulose. If we include in hydrolase mixture construction the ligninase involved in lignin degradation, which mainly includes laccase, manganese peroxidases (MnP) and lignin peroxidase (LiP), it is feasible that this could greatly improve the fermentable sugars yield.ResultsThe psychrophilic lignocellulosic enzymes system of Cladosporium cladosporioides Ch2-2 including ligninase and polysaccharide hydrolases was suitable for selective delignification and efficient saccharification of biomass with wide thermal adaptability. The purified laccase was optimally active at 15°C and pH 3.5, exhibiting high thermostability over a broad range of temperatures (between 4 and 40°C). In addition, manganese-independent peroxidase (MIP), a special type of ligninase with the capacity to oxidize dimethyl phthalate (DMP) in the absence of H2O2 and Mn2+, was optimally active at 20°C and pH 2.5, exhibiting high thermostability over a broad range of temperatures (4 and 28°C), while depressed completely by Fe2+ and essentially unaffected by EDTA. Synergy between Ch2-2 crude enzymes and commercial xylanase obviously enhanced biomass hydrolysis, which could take the place of expensive commercial cellulase mixture. The maximum value of synergistic degree reached 4.7 at 28°C, resulting in 10.1 mg/mL reducing sugars.ConclusionsThe psychrophilic enzymes system of C. cladosporioides Ch2-2 with a different synergistic mechanism has huge potential for the enhancement of biomass hydrolysis at mesophilic and low temperatures. The application scope of the lignocellulosic enzyme cocktail could be greatly enlarged by optimizing the operation conditions specific to the characteristics of ligninase.

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“…3c and d). 34 Aer determining the saturation limits for each of the four enzyme supplements, optimization of the enzyme mixture was performed by response surface methodology. Hu, et al 28 and Li, et al 30 have also found that hydrolysis yield was increased by approx.…”

Section: Supplementation Of Cellulase With Accessory Enzymesmentioning

confidence: 99%

“…Recently, hydrolysis of date palm lignocellulosic waste was improved to 60% by synergistic effect of cellulase, xylanase and laccase in comparison of cellulase alone. 34 Aer determining the saturation limits for each of the four enzyme supplements, optimization of the enzyme mixture was performed by response surface methodology.…”

Section: Supplementation Of Cellulase With Accessory Enzymesmentioning

confidence: 99%

Improved saccharification of pilot-scale acid pretreated wheat straw by exploiting the synergistic behavior of lignocellulose degrading enzymes

et al. 2015

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Requirement of high enzyme dosage for lignocellulosic biomass hydrolysis is one of the challenges for the viability of the second generation bioethanol technology. Here, an optimal enzyme mixture was developed by partially replacing the cellulase proportion with accessory enzymes (b-glucosidase, xylanase, pectinase, laccase) and its hydrolytic performance was compared with different commercial counterparts for the saccharification of pretreated wheat straw (PWS) using a 250 kg per day continuous pilot plant. Maximum degree of synergism was observed with xylanase followed by pectinase, laccase, and bglucosidase. The statistically optimized enzyme mixture enhanced hydrolysis by 51.23% and 40.66% in 6 h and 24 h, respectively. This study elucidates that presence of even small amount of oligomers and cellobiose pose a strong inhibition for the enzymes. Therefore, development of an optimal enzyme formulation is a sustainable approach to reduce overall enzyme loading for biomass saccharification.

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Synergistic effect of pretreatment and hydrolysis enzymes on the production of fermentable sugars from date palm lignocellulosic waste

Cited by 29 publications

References 15 publications

Biofuels and Biochemicals Production

Biofuels and Biochemicals Production

Synergy of crude enzyme cocktail from cold-adapted Cladosporium cladosporioides Ch2-2 with commercial xylanase achieving high sugars yield at low cost

Improved saccharification of pilot-scale acid pretreated wheat straw by exploiting the synergistic behavior of lignocellulose degrading enzymes

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