Synergistic effect of rapamycin and metformin against germ cell apoptosis and oxidative stress after testicular torsion/detorsion-induced ischemia/reperfusion in rats

Ghasemnejad‐Berenji, Morteza; Ghazi‐Khansari, Mahmoud; Pashapour, Sarvin; Jafari, Abbas Ali; Yazdani, Iraj; Ghasemnejad-berenji, Hojjat; Saravi, Seyed Soheil Saeedi; Sadeghpour, Sonia; Nobakht, Maliheh; Abdollahi, Alireza; Ansari, Javad Mohajer; Dehpour, Ahmad Reza

doi:10.1016/j.biopha.2018.06.012

Cited by 34 publications

(30 citation statements)

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“…The metformin dose, we administered was presented and shown to be efficiency in a previous study (Ghasemnejad-Berenji, Ghazi-Khansari, Pashapour, et al, 2018). The metformin dose, we administered was presented and shown to be efficiency in a previous study (Ghasemnejad-Berenji, Ghazi-Khansari, Pashapour, et al, 2018).…”

Section: Experimental Groups and Surgical Proceduresmentioning

confidence: 91%

“…Each group consisted of eight rats per group The dose of metformin used was 100 mg/kg in two studies (Asghari et al, 2016a(Asghari et al, , 2016b and 300 mg/ml in the recent study (Ghasemnejad-Berenji, Ghazi-Khansari, Pashapour, et al, 2018;. Ischaemia/reperfusion with metformin (I/R + M) group had significantly lower score than I/R group (p < .001).…”

Section: F I G U R Ementioning

confidence: 99%

“…Metformin can stimulate AMPK production and modulate the nutritional function of Sertoli cells (Galardo, Riera, Pellizzari, Cigorraga, & Meroni, 2007). Three previous studies reported the beneficial effects of metformin following I/R in adult rat testis, restoring antioxidant activities and attenuating apoptosis (Asghari, Akbari, Meghdadi, & Mortazavi, 2016a, 2016bGhasemnejad-Berenji, Ghazi-Khansari, Pashapour, et al, 2018;Sengupta, 2013). It was also reported that the presence of metformin in the cryopreservation fluid augmented the fertilising capacity of the rat spermatozoa and lowered the proportion of abnormal embryos through IVF (Bertoldo, Guibert, Tartarin, Guillory, & Froment, 2014).…”

Section: Introductionmentioning

confidence: 98%

“…It was also reported that the presence of metformin in the cryopreservation fluid augmented the fertilising capacity of the rat spermatozoa and lowered the proportion of abnormal embryos through IVF (Bertoldo, Guibert, Tartarin, Guillory, & Froment, 2014). Three previous studies reported the beneficial effects of metformin following I/R in adult rat testis, restoring antioxidant activities and attenuating apoptosis (Asghari, Akbari, Meghdadi, & Mortazavi, 2016a, 2016bGhasemnejad-Berenji, Ghazi-Khansari, Pashapour, et al, 2018;Sengupta, 2013).…”

mentioning

confidence: 94%

“…,Ghazi-Khansari, Pashapour, et al (2018) and assessed MDA, caspase-3, catalase, SOD and glutathione peroxidase levels 4 hr after detorsion following a 1 hr of torsion of the right testis. DNA strand breaks and seminiferous tubule diameters were examined 24 hr later, and the sperm concentration and motility were examined 30 days after I/R.…”

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confidence: 99%

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Metformin decreases testicular damages following ischaemia/reperfusion injury in rats

et al. 2019

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The effects of metformin on a testicular torsion injury in adolescent rat testis after I/R were evaluated in the present study. Forty adolescent rats were divided into five groups with eight rats per group: a control group; a sham‐operated group; an ischaemia group, where torsion was applied for 4 hr and testis was examined immediately after detorsion; an I/R group, where torsion was applied for 4 hr and the testis was examined 4 hr after detorsion; and an I/R + M group, where the metformin (300 mg/kg) administration was added to the identical procedures used for the I/R group. Spermatogenesis, basal membrane integrity and cleaved caspase‐3 expression were assessed. The I/R + M group had a significantly higher Johnsen score than the I/R group (7.9 ± 0.1 vs. 7.5 ± 0.2; p < .001; F‐value = 14.2). Failure of basal membrane integrity was highest in the ischaemia group (45 ± 5) compared to the other groups (control group, 20 ± 5; sham‐operated group, 16.6 ± 2.8), but not different between the I/R + M (31.6 ± 12.5) and the I/R groups (25 ± 3.5). Cleaved caspase‐3 expression was highest in the ischaemia group (73.5 ± 0.7), and significantly lower in the I/R + M group (33.4 ± 0.9) than the I/R group (58.5 ± 0.2; p < .05; F‐value = 7.6). Metformin decreases testicular damage by exerting protection against the harmful effects of I/R on spermatogenesis and alleviating apoptosis in adolescent rat testis.

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Section: Experimental Groups and Surgical Proceduresmentioning

confidence: 91%

Section: F I G U R Ementioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

mentioning

confidence: 94%

mentioning

confidence: 99%

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Metformin decreases testicular damages following ischaemia/reperfusion injury in rats

et al. 2019

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Daphnetin protects hippocampal neurons from oxygen‐glucose deprivation–induced injury

Jin

Duan

Pei

et al. 2018

J of Cellular Biochemistry

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Daphnetin, a coumarin derivative extracted from Daphne odora var., was reported to possess a neuroprotective effect. Recently, it has been demonstrated that daphnetin attenuates ischemia/reperfusion (I/R) injury. However, the role of daphnetin in cerebral I/R injury and the potential mechanism have not been fully understood. The present study aimed to explore the regulatory roles of daphnetin on oxygen-glucose deprivation/reoxygenation (OGD/R)-induced cell injury in a model of hippocampal neurons. Our results demonstrated that daphnetin improved cell viability and reduced the lactate dehydrogenase leakage in OGD/R-stimulated hippocampal neurons. In addition, daphnetin inhibited oxidative stress and cell apoptosis in hippocampal neurons after OGD/ R stimulation. Furthermore, daphnetin significantly enhanced the nuclear translocation of the nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) expression in hippocampal neurons exposed to OGD/R. Knockdown of Nrf2 blocked the protective effect of daphnetin on OGD/ R-induced hippocampal neurons. In conclusion, these findings demonstrated that daphnetin attenuated oxidative stress and neuronal apoptosis after OGD/R injury through the activation of the Nrf2/HO-1 signaling pathway in hippocampal neurons. Thus, daphnetin may be a novel therapeutic agent for cerebral I/R injury. K E Y W O R D S cerebral ischemia/reperfusion (I/R) injury, daphnetin, hippocampal neurons, neuroprotective, Nrf2/HO-1 pathway, oxidative stress How to cite this article: Zhi J, Duan B, Pei J, Wu S, Wei J. Daphnetin protects hippocampal neurons from oxygen-glucose deprivation-induced injury.

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AMPK: The key to ischemia‐reperfusion injury

Cai

Chen

Liu

et al. 2022

Journal Cellular Physiology

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Ischemia-reperfusion injury (IRI) refers to a syndrome in which tissue damage is further aggravated and organ function further deteriorates when blood flow is restored after a period of tissue ischemia. Acute myocardial infarction, stress ulcer, pancreatitis, intestinal ischemia, intermittent claudication, acute tubular necrosis, postshock liver failure, and multisystem organ failure are all related to reperfusion injury. AMP-activated protein kinase (AMPK) has been identified in multiple catabolic and anabolic signaling pathways. The functions of AMPK during health and diseases are intriguing but still need further research. Except for its conventional roles as an intracellular energy switch, emerging evidence reveals the critical role of AMPK in IRI as an energy-sensing signal molecule by regulating metabolism, autophagy, oxidative stress, inflammation, and other progressions. At the same time, drugs based on AMPK for the treatment of IRI are constantly being researched and applied in clinics. In this review, we summarize the mechanisms underlying the effects of AMPK in IRI and describe the AMPK-targeting drugs in treatment, hoping to increase the understanding of AMPK in IRI and provide new insights into future clinical treatment.

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Synergistic effect of rapamycin and metformin against germ cell apoptosis and oxidative stress after testicular torsion/detorsion-induced ischemia/reperfusion in rats

Cited by 34 publications

References 40 publications

Metformin decreases testicular damages following ischaemia/reperfusion injury in rats

Metformin decreases testicular damages following ischaemia/reperfusion injury in rats

Daphnetin protects hippocampal neurons from oxygen‐glucose deprivation–induced injury

AMPK: The key to ischemia‐reperfusion injury

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