Synergistic Effect of TGF-β1 And BMP–7 on Chondrogenesis and Extracellular Matrix Synthesis: An In Vitro Study

Gökçe, Alper; Yılmaz, İbrahim; Bircan, Rıfat; Tonbul, Murat; Gokay, Nevzat Selim; Gökçe, Çiğdem

doi:10.2174/1874325001206010406

Cited by 34 publications

(43 citation statements)

References 29 publications

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“…Some studies suggested that BMP- 2 had the ability to facilitate cartilage formation by chondrocytes and MSCs for treatment of cartilage and osteochondral defects [45, 249, 250]. BMP- 7 was used in cell-laden hydrogel constructs for promoting chondrogenesis and cartilage ECM formation [251]. Extensive research has shown that BMPs are effective growth factors to regulate both cartilage and bone formation.…”

Section: Effects Of Growth Factors and Delivery Methods On Chondromentioning

confidence: 99%

Cell-laden hydrogels for osteochondral and cartilage tissue engineering

et al. 2017

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Despite tremendous advances in the field of regenerative medicine, it still remains challenging to repair the osteochondral interface and full-thickness articular cartilage defects. This inefficiency largely originates from the lack of appropriate tissue engineered artificial matrices that can replace the damaged regions and promote tissue regeneration. Hydrogels are emerging as a promising class of biomaterials for both soft and hard tissue regeneration. Many critical properties of hydrogels, such as mechanical stiffness, elasticity, water content, bioactivity, and degradation, can be rationally designed and conveniently tuned by proper selection of the material and chemistry. Particularly, advances in the development of cell-laden hydrogels have opened up new possibilities for cell therapy. In this article, we describe the problems encountered in this field and review recent progress in designing cell-hydrogel hybrid constructs for promoting the reestablishment of osteochondral/cartilage tissues. Our focus centers on the effects of hydrogel type, cell type, and growth factor delivery on achieving efficient chondrogenesis and osteogenesis. We give our perspective on developing next-generation matrices with improved physical and biological properties for osteochondral/cartilage tissue engineering. We also highlight recent advances in biomanufacturing technologies (e.g. molding, bioprinting, and assembly) for fabrication of hydrogel-based osteochondral and cartilage constructs with complex compositions and microarchitectures to mimic their native counterparts.

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Section: Effects Of Growth Factors and Delivery Methods On Chondromentioning

confidence: 99%

Cell-laden hydrogels for osteochondral and cartilage tissue engineering

et al. 2017

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“…MTT analyses were evaluated in accordance with commercial kit bulletins on the 1st, 7th, 14th, and 21st days [15–20, 24, 26]. …”

Section: Methodsmentioning

confidence: 99%

Can a Biodegradable Implanted Bilayered Drug Delivery System Loaded with BMP-2/BMP-12 Take an Effective Role in the Biological Repair Process of Bone–Tendon Injuries? A Preliminary Report

Kömür

Akyuva

Karaslan

et al. 2017

Journal of Pharmaceutics

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Background Use of biodegradable and biocompatible materials in the orthopedic surgery is gaining popularity. In this research, the rate of controlled release of a bilayered prototype biomaterial designed to promote osteoblastic and tenoblastic activity was calculated using pharmacochemical methods. Methods The first part of the design, composed of a sodium tetraborate, polyvinyl alcohol, and starch based hydrogel, was loaded with bone morphogenic protein-2. The second part which was composed of a sodium tetraborate, polyvinyl alcohol, and chitosan based hydrogel was loaded with bone morphogenic protein-12. Osteochondral and tendon tissue specimens were obtained from patients with a diagnosis of gonarthrosis and primary bone cells and tendon cells cultures were prepared following treatment with collagenase enzyme. Cell samples were collected from the groups by means of an invert light microscope and environmental scanning electron microscope underwent at the 1st and 21st days. The level of osteogenic differentiation was measured by the activity of alkaline phosphatase. For the statistical evaluation of the obtained data, groups were compared with post hoc Tukey test following analysis of variance. Level of significance was accepted to be <0,01. Results Both osteogenic and tenogenic stimulation were observed in the cultured specimens. In comparison to the control groups, the rate of proliferation of healthy cells was found to be higher in the groups to which the design was added (p < 0.01). Conclusions Our research is a preliminary report that describes a study conducted in an in vitro experimental setting. We believe that such prototype systems may be pioneers in targeted drug therapies after reconstructional surgeries.

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“…The rhOP‐1 release from the scaffolds over a 28‐day period was evaluated using an enzyme‐linked immunosorbent assay (ELISA) kit # . The OP‐1 ELISA was performed according to an established protocol in 96‐well plates with two control columns 24 . Absorbance was measured at 400 nm, and the result was compared with the control curve to give the OP‐1 concentration at each time period.…”

Section: Methodsmentioning

confidence: 99%