Synergistic Effects of a Tomato chlorosis virus and Tomato yellow leaf curl virus Mixed Infection on Host Tomato Plants and the Whitefly Vector

Li, Jie; Wang, Jicheng; Ding, Tian-Bo; Chu, Dong

doi:10.3389/fpls.2021.672400

Cited by 27 publications

(17 citation statements)

References 55 publications

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“…In mixed infections, many begomoviruses co-existing in natural host plants have shown neutral synergistic interactions [ 50 , 51 , 52 ], which may lead to more severe disease complexes. The finding that tomato and muskmelon plants harbor a mixed infection of CLCuGeV and its associated DNA-satellites together with TYLCV or ToLCPalV might suggest that these viruses may complement each other, as is the case of previous reports on TYLCV [ 50 , 53 , 54 ]. Nevertheless, the association of OLCuOMB and OYCrCMA might be a host-switching strategy because CLCuGeV is mostly associated with CLCuGeB and/or CLCuGeA [ 19 , 34 , 55 ].…”

Section: Discussionmentioning

confidence: 75%

High-Throughput Sequencing Identified Distinct Bipartite and Monopartite Begomovirus Variants Associated with DNA-Satellites from Tomato and Muskmelon Plants in Saudi Arabia

Alhudaib

Almaghasla

El-Ganainy

et al. 2022

Plants

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The studies on the prevalence and genetic diversity of begomoviruses in Saudi Arabia are minimal. In this study, field-grown symptomatic tomato and muskmelon plants were collected, and initially, begomovirus infection was confirmed by the core coat protein sequences. Four tomato and two muskmelon plants with viral infections were further evaluated for Illumina MiSeq sequencing, and twelve sequences (2.7–2.8 kb) equivalent to the full-length DNA-A or DNA-B components of begomoviruses were obtained along with eight sequences (~1.3–1.4 kb) equivalent to the begomovirus-associated DNA-satellite components. Four begomovirus sequences obtained from tomato plants were variants of tomato yellow leaf curl virus (TYLCV) with nt sequence identities of 95.3–100%. Additionally, two tomato plants showed a mixed infection of TYLCV and cotton leaf curl Gezira virus (CLCuGeV), okra yellow crinkle Cameroon alphasatellite (OYCrCMA), and okra leaf curl Oman betasatellite (OLCuOMB). Meanwhile, from muskmelon plants, two sequences were closely related (99–99.6%) to the tomato leaf curl Palampur virus (ToLCPalV) DNA-A, whereas two other sequences showed 97.9-100% sequence identities to DNA-B of ToLCPalV, respectively. Complete genome sequences of CLCuGeV and associated DNA-satellites were also obtained from these muskmelon plants. The nt sequence identities of the CLCuGeV, OYCrCMA, and OLCuOMB isolates obtained were 98.3–100%, 99.5–100%, and 95.6–99.7% with their respective available variants. The recombination was only detected in TYLCV and OLCuOMB isolates. To our knowledge, this is the first identification of a mixed infection of bipartite and monopartite begomoviruses associated with DNA-satellites from tomato and muskmelon in Saudi Arabia. The begomovirus variants reported in this study were clustered with Iranian isolates of respective begomovirus components in the phylogenetic dendrogram. Thus, the Iranian agroecological route can be a possible introduction of these begomoviruses and/or their associated DNA-satellites into Saudi Arabia.

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Section: Discussionmentioning

confidence: 75%

High-Throughput Sequencing Identified Distinct Bipartite and Monopartite Begomovirus Variants Associated with DNA-Satellites from Tomato and Muskmelon Plants in Saudi Arabia

Alhudaib

Almaghasla

El-Ganainy

et al. 2022

Plants

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“…Thus, we believe that this is a case of synergistic interaction. Li et al (2021) found that ToCV and TYLCV mixed-infected tomato plants had a high disease severity index, and ToCV and TYLCV mixed-infected plants with viral accumulation was greater than in singly infected plants ( Li et al, 2021 ). We hypothesize that the ability of whiteflies to acquire and transmit ToCV significantly increased after the co-infection of TYLCV and ToCV by a whitefly protein interacting with ToCV proteins.…”

Section: Discussionmentioning

confidence: 99%

Co-infection of TYLCV and ToCV increases cathepsin B and promotes ToCV transmission by Bemisia tabaci MED

Liao

Fajar

et al. 2023

Front. Microbiol.

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Tomato disease is an important disease affecting agricultural production, and the combined infection of tomato chlorosis virus (ToCV) and tomato yellow leaf curl virus (TYLCV) has gradually expanded in recent years, but no effective control method has been developed to date. Both viruses are transmitted by Bemisia tabaci Mediteranean (MED). Previously, we found that after B. tabaci MED was fed on ToCV-and TYLCV-infected plants, the transmission efficiency of ToCV was significantly higher than that on plants infected only with ToCV. Therefore, we hypothesize that co-infection could enhance the transmission rates of the virus. In this study, transcriptome sequencing was performed to compare the changes of related transcription factors in B. tabaci MED co-infected with ToCV and TYLCV and infected only with ToCV. Hence, transmission experiments were carried out using B. tabaci MED to clarify the role of cathepsin in virus transmission. The gene expression level and enzyme activity of cathepsin B (Cath B) in B. tabaci MED co-infected with ToCV and TYLCV increased compared with those under ToCV infection alone. After the decrease in cathepsin activity in B. tabaci MED or cathepsin B was silenced, its ability to acquire and transmit ToCV was significantly reduced. We verified the hypothesis that the relative expression of cathepsin B was reduced, which helped reduce ToCV transmission by B. tabaci MED. Therefore, it was speculated that cathepsin has profound research significance in the control of B. tabaci MED and the spread of viral diseases.

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“…The expression of ToCV minor coat protein (CPm) gene was then measured using reverse transcription quantitative real-time PCR (RT-qPCR) to evaluate ToCV loads in tomato seedlings. 29,30 In the beginning, the influences of two factors (the number of adults and the detection time) on the transmission efficiency of ToCV were investigated. The ToCV isolate was collected from one tomato field in Qingdao, Shandong, China and kept on tomato plants in the laboratory conditions described above.…”

Section: Effects Of Trpv Channel Modulators On Tocv Transmissionmentioning

confidence: 99%

“…The ToCV isolate was collected from one tomato field in Qingdao, Shandong, China and kept on tomato plants in the laboratory conditions described above. 30 After 3 weeks post-inoculation (wpi), ToCV-infected tomato plants were used as viral sources and non-infected tomato plants were used as negative controls. Because female B. tabaci adults could acquire maximal viral loads in 48-h acquisition access periods (AAPs), 31 nonviruliferous whitefly female adults fed on ToCV-infected tomato plants for 48 h and were used as viruliferous whitefly in this study.…”

Section: Effects Of Trpv Channel Modulators On Tocv Transmissionmentioning

confidence: 99%

Pyrifluquinazon baseline susceptibility and inhibition of Tomato chlorosis virus transmission by Bemisia tabaci

Wen

Feng

Zhu

et al. 2023

Pest Management Science

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BackgroundTomato chlorosis virus (ToCV) is associated with tomato yellow leaf disorder diseases in more than 20 countries. ToCV can be transmitted in a semipersistent manner by whitefly vectors such as Bemisia tabaci. Controlling the vector pests by using chemical insecticides is an efficient and effective approach to reduce and interrupt the virus transmission. Pyrifluquinazon is a new pyridine azomethine derivative, showing insecticidal toxicity to sucking pests by disturbing their feeding behavior. However, limited attention has been paid to the performance of pyrifluquinazon against B. tabaci and ToCV transmission.ResultThis study showed the lethal concentration of 50% (LC50) values of pyrifluquinazon to 22 B. tabaci field populations ranged from 0.54 to 2.44 mg L−1, and the baseline susceptibility of B. tabaci to pyrifluquinazon was 1.24 mg L−1 with a 95% confidence limit of 0.35–1.85 mg L−1. Pyrifluquinazon and afidopyropen did not show cross‐resistance to dinotefuran and pymetrozine in B. tabaci, which both inhibited the feeding activities of B. tabaci. The antifeedant concentration of 50% (AFC50) values at 48 h were 0.70 mg L−1 for pyrifluquinazon and 2.13 mg L−1 for afidopyropen. Foliar application of pyrifluquinazon and afidopyropen reduced the ToCV transmission by 40.91% and 33.33%, respectively and significantly decreased the ToCV loads in tomato plants under laboratory conditions.ConclusionThese results provided new information about the effects of modulators of the vanilloid‐type transient receptor potential channel on the toxicity to B. tabaci and inhibition of ToCV transmission. © 2023 Society of Chemical Industry.

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Synergistic Effects of a Tomato chlorosis virus and Tomato yellow leaf curl virus Mixed Infection on Host Tomato Plants and the Whitefly Vector

Cited by 27 publications

References 55 publications

High-Throughput Sequencing Identified Distinct Bipartite and Monopartite Begomovirus Variants Associated with DNA-Satellites from Tomato and Muskmelon Plants in Saudi Arabia

High-Throughput Sequencing Identified Distinct Bipartite and Monopartite Begomovirus Variants Associated with DNA-Satellites from Tomato and Muskmelon Plants in Saudi Arabia

Co-infection of TYLCV and ToCV increases cathepsin B and promotes ToCV transmission by Bemisia tabaci MED

Pyrifluquinazon baseline susceptibility and inhibition of Tomato chlorosis virus transmission by Bemisia tabaci

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