Synergistic effects of Akt1 shRNA and paclitaxel-incorporated conjugated linoleic acid-coupled poloxamer thermosensitive hydrogel on breast cancer

Guo, Ding‐Ding; Hong, Sun; Jiang, Hu‐Lin; Kim, Jihye; Minai-Tehrani, Arash; Kim, Jieun; Shin, Ji‐Young; Jiang, Tao; Kim, You-Kyoung; Choi, Yun‐Jaie; Cho, Chong‐Su; Cho, Myung‐Haing

doi:10.1016/j.biomaterials.2011.12.011

Cited by 46 publications

(32 citation statements)

References 44 publications

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“…Next, it was important to examine the therapeutic potential in vivo ; previous studies examining the potential of local tumour therapy have mostly used microparticles in non-breast tumours [13]. A few studies have used gels [8, 10, 12, 35–38] or polymer drug conjugates [39–41], and no studies have been reported for films. For the first time, we have used a humanised orthotopic breast cancer model that allowed us to examine both locoregional control, and its impact on metastasis following local therapy.…”

Section: Discussionmentioning

confidence: 99%

Doxorubicin-loaded silk films: Drug-silk interactions and in vivo performance in human orthotopic breast cancer

2012

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Breast cancer is the most common of all malignant diseases in women. Systemic chemotherapy provides low clinical benefit for locoregional control of the disease, while localised chemotherapy may provide a therapeutic advantage. In this study, doxorubicin-loaded silk films were directly applied to tumours. Affinity binding studies demonstrated that the adsorption of doxorubicin onto silk was partially dependent on crystallinity. By manipulating silk crystallinity, or β-sheet content, the doxorubicin release rate could be controlled ranging from immediate release to prolonged release over >4 weeks. Following successful in vitro studies, the therapeutic impact of doxorubicin-loaded silk films on primary tumour growth and metastasis was assessed in mice using a humanised orthotopic breast cancer model (adenocarcinoma). Both soluble and stabilised silk films loaded with doxorubicin had a significantly greater primary tumour response than the equivalent dose of doxorubicin administered intravenously in the absence of the silk film carrier. In addition to reducing primary tumour growth, stabilised silk films loaded with doxorubicin also reduced metastatic spread and autopsy indicated that these films were not associated with any local or systemic toxicities. Collectively, these results suggest that the future use of this approach for localised chemotherapy is promising.

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Section: Discussionmentioning

confidence: 99%

Doxorubicin-loaded silk films: Drug-silk interactions and in vivo performance in human orthotopic breast cancer

2012

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“…For example, bladder cancer is often treated with intravesical chemotherapy and high-grade malignant glioma is treated by placing carmustine-containing synthetic copolymer wafers (Gliadel® Wafer) at the site of tumor resection [6, 7] . A number of polyethylene glycol (PEG) hydrogel-based systems that are tailored to respond to a stimulus such as temperature, ionic strength or pH [8–12] to trigger controlled drug release are in preclinical development. Other hydrogel systems include poly(organophosphazane) [8] polymers, PLGA-PEG-PLGA triblock compolymers [10] or poly(ester-carbonate)-composites [13] .…”

Section: Introductionmentioning

confidence: 99%

Self‐Assembling Doxorubicin Silk Hydrogels for the Focal Treatment of Primary Breast Cancer

Seib

Pritchard

Kaplan

2012

Adv Funct Materials

138

146

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Standard care for early stage breast cancer includes tumor resection and local radiotherapy to achieve long-term remission. Systemic chemotherapy provides only low locoregional control of the disease; therefore, we describe self-assembling silk hydrogels that can retain and then deliver doxorubicin locally. Self-assembling silk hydrogels show no swelling, are readily loaded with doxorubicin under aqueous conditions and release drug over 4 weeks in amounts that can be fine-tuned by varying the silk content. Following successful in vitro studies, locally injected silk hydrogels loaded with doxorubicin show excellent antitumor response in mice, outperforming the equivalent amount of doxorubicin delivered intravenously. In addition to reducing primary tumor growth, doxorubicin-loaded silk hydrogels reduce metastatic spread and are well tolerated in vivo. Thus, silk hydrogels are well suited for the local delivery of chemotherapy and provide a promising approach to improve locoregional control of breast cancer.

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“…A report from Guo et al later demonstrated hydrogel‐mediated, local gene silencing through shRNA DNA plasmid transfection for cancer therapy . Here, linear PEI was modified with PEG to complex DNA for transfection.…”

Section: In Vivo Applications Of Hydrogel Rnai Delivery Systemsmentioning

confidence: 95%

Engineered Hydrogels for Local and Sustained Delivery of RNA‐Interference Therapies

Wang

Burdick

2016

Adv Healthcare Materials

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It has been nearly two decades since RNA-interference (RNAi) was first reported. While there are no approved clinical uses, several promising phase II and III clinical trials suggest the great promise of RNAi therapeutics. One challenge for RNAi therapies is the controlled localization and sustained presentation to target tissues, to both overcome systemic toxicity concerns and to enhance in vivo efficacy. One approach that is emerging to address these limitations is the entrapment of RNAi molecules within hydrogels for local and sustained release. In these systems, nucleic acids are either delivered as siRNA conjugates or within nanoparticles. A plethora of hydrogels has been implemented using these approaches, including both traditional hydrogels that have already been developed for other applications and new hydrogels developed specifically for RNAi delivery. These hydrogels have been applied to various applications in vivo, including cancer, bone regeneration, inflammation and cardiac repair. This review will examine the design and implementation of such hydrogel RNAi systems and will cover the most recent applications of these systems.

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Synergistic effects of Akt1 shRNA and paclitaxel-incorporated conjugated linoleic acid-coupled poloxamer thermosensitive hydrogel on breast cancer

Cited by 46 publications

References 44 publications

Doxorubicin-loaded silk films: Drug-silk interactions and in vivo performance in human orthotopic breast cancer

Doxorubicin-loaded silk films: Drug-silk interactions and in vivo performance in human orthotopic breast cancer

Self‐Assembling Doxorubicin Silk Hydrogels for the Focal Treatment of Primary Breast Cancer

Engineered Hydrogels for Local and Sustained Delivery of RNA‐Interference Therapies

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