Synergistic effects of apelin and leptin on isolated rat pulmonary arteries

Dumitriu, Irina Luciana; Vata, L.G.; Zugun, F; Gurzu, Bogdan

doi:10.2478/s11536-011-0033-1

Cited by 1 publication

(5 citation statements)

References 36 publications

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“…In agreement with literature published data, our previous results showed that LEP could have vasodilatatory effects on Phe precontracted pulmonary arteries from normal rats but not on sensitized rats (11). In this study the LEP was administered before Phe.…”

Section: Discussionsupporting

confidence: 91%

“…injection of 0.2 mL physiological saline, containing 100 mg OVA and 8 mg aluminium hydroxide. The protocol was repeated 2 weeks later (11,12). In vitro L.G.…”

Section: Methodsmentioning

confidence: 99%

“…2-7.4). A resting tension of 0.3g for PA was applied to each ring and then allowed to equilibrate for 45-60 minutes before starting the experiment as previously described (11). The bathing medium was renewed every 15 minutes.…”

Section: Methodsmentioning

confidence: 99%

“…The rings that produced less than 50% relaxation in response to ACh were discarded. After re-equilibration in Krebs solution for 45 min, we administered leptin (LEP, 10 ng/mL), angiotensinogen (Aogen, 10 µM) and apelin 13 (AP13, 1 µM) in doses that did not modify the basal tone (11,12). After 10 minutes, 1 µM Phe was administered and the contraction was measured on plateau phase as percentage from control contraction 40 mM KCl.…”

Section: Methodsmentioning

confidence: 99%

“…On the other hand, taking into account both the role of obesity in the development of pulmonary hypertension and our data about adipokines contribution on pulmonary vessels reactivity on healthy and sensitized rats (11) we studied the effects of some adipokines (leptin, apelin, angiotensinogen) on contractile responses of pulmonary arteries from obese resistant rats fed with a HFD. Our aim was to assess the interaction between HFD and adipokines on isolated pulmonary arteries from healthy and ovalbumin sensitized rat, in the absence of obesity.…”

Section: Introductionmentioning

confidence: 99%

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Obesity Independent Effects of High Fat Diet on Pulmonary Arteries Reactivity

Vata¹

2011

Acta Endo (Buc)

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Published data sustain the involvement of diet on pathogenesis of immune-mediated diseases.Aim. To determine if the high fat diet (HFD), in the absence of obesity, could modulate the altered pulmonary arteries reactivity associated to allergic lung diseases. Materials and Methods. Obese resistant rats were divided into 2 groups: standard chow diet and HFD. Randomly chosen rats from both groups were sensitized against ovalbumin. The histological aspect and reactivity of pulmonary arteries were comparatively assessed. Taking into account the involvement of adipokines on obesity associated vascular reactivity alteration we also studied the vasomotor effects of few adipokines on pulmonary vessels.Results. Lung histological examination revealed that HFD aggravated the remodelling of pulmonary arteries and inflammation of lung parenchyma. The HFD amplified the phenylephrine -induced contractions. Angiotensinogen amplified and apelin inhibited the Phe contractile effects on sensitized HFD fed rats.Conclusion.These effects could be at least mediated, by both the alteration of adipokines vasomotor effects and inflammation associated to pulmonary allergic disease.

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Section: Discussionsupporting

confidence: 91%

“…injection of 0.2 mL physiological saline, containing 100 mg OVA and 8 mg aluminium hydroxide. The protocol was repeated 2 weeks later (11,12). In vitro L.G.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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