Synergistic Effects of Apigenin and Paclitaxel on Apoptosis of Cancer Cells

Xu, Yuekang; Xin, Yinqiang; Diao, Youxiang; Lu, Changyan; Fu, Jin; Luo, Lan; Yin, Zhimin

doi:10.1371/journal.pone.0029169

Cited by 124 publications

(102 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also, the clinical use of current treatment modalities is often hampered by severe side effects and toxicity to normal tissues (Xu et al, 2008;Xu et al, 2011;Singh et al, 2013). Therefore, considering the high incidence and mortality rate of cervical cancer worldwide novel treatment strategies are urgently required Xu et al, 2008;Singh et al, 2013).…”

Section: Sensitization Of Cervical Cancer Cells To Chemo-and/or Radiomentioning

confidence: 99%

See 1 more Smart Citation

Characteristic Features of Cytotoxic Activity of Flavonoids on Human Cervical Cancer Cells

Sak¹

2014

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

Cervical cancer is the most common gynecologic malignancy worldwide and development of new therapeutic strategies and anticancer agents is an urgent priority. Plants have remained an important source in the search for novel cytotoxic compounds and several polyphenolic flavonoids possess antitumor properties. In this review article, data about potential anticarcinogenic activity of common natural flavonoids on various human cervical cancer cell lines are compiled and analyzed showing perspectives for the use of these secondary metabolites in the treatment of cervical carcinoma as well as in the development of novel chemotherapeutic drugs. Such anticancer effects of flavonoids seem to differentially depend on the cellular type and origin of cervical carcinoma creating possibilities for specific targeting in the future. Besides the cytotoxic activity per se, several flavonoids can also contribute to the increase in efficacy of conventional therapies rendering tumor cells more sensitive to standard chemotherapeutics and irradiation. Although the current knowledge is still rather scarce and further studies are certainly needed, it is clear that natural flavonoids may have a great potential to benefit cervical cancer patients.

show abstract

Section: Sensitization Of Cervical Cancer Cells To Chemo-and/or Radiomentioning

confidence: 99%

“…Such combined use might improve the efficiency of paclitaxel as a chemotherapeutic drug and could be an effective way to reduce the dosage of paclitaxel in cancer therapy accompanied by a decrease in its harmful side effects (Xu et al, 2011).…”

Section: Sensitization Of Cervical Cancer Cells To Standard Chemothermentioning

confidence: 99%

Characteristic Features of Cytotoxic Activity of Flavonoids on Human Cervical Cancer Cells

Sak¹

2014

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

show abstract

“…Apigenin has additionally been reported to induce apoptosis of prostate and colon cancer cells through induction of death receptor 5, and to act synergistically with exogenous tumor necrosis factor-related apoptosis-inducing ligand to induce cell apoptosis (12). Furthermore, apigenin enhances the anticancer activity or minimizes the resistance of cancer cells to a number chemotherapeutics, including gemcitabine, paclitaxel, 5-fluorouracil and doxorubicin by inducing apoptosis (13)(14)(15)(16)(17). It was reported that apigenin sensitizes neuroblastoma cells to the anticancer drug etoposide by retention of p53 in cell nuclei (18).…”

Section: Introductionmentioning

confidence: 99%

Apigenin enhances the cisplatin cytotoxic effect through p53-modulated apoptosis

Liu

et al. 2016

Oncology Letters

View full text Add to dashboard Cite

Abstract. Epidemiological and experimental evidence suggests that dietary flavonoids, including apigenin, have anticancer roles. Apigenin has been reported to elevate p53, a critical molecule in the induction of apoptosis. The present study aimed to investigate whether apigenin, a dietary flavonoid, improves the cytotoxic effect of cisplatin in a cancer cell culture system, and to elucidate the mechanism of this effect. Multiple tumor cell types were treated with apigenin, cisplatin or both drugs. Cell viability was evaluated, and the cytotoxic effect was determined biochemically and microscopically. Treatment with apigenin increased cisplatin-induced DNA damage and the apoptosis of tumor cells in a p53-dependent manner. Apigenin, when used with cisplatin, inhibited cell proliferation and promoted mitogen-activated protein kinase activation and subsequent p53 phosphorylation, leading to p53 accumulation and upregulation of proapoptotic proteins. Cisplatin is one of the most commonly used chemotherapeutic drugs for malignant tumors, but resistance to this drug occurs. The current results therefore demonstrate that dietary flavonoids may diminish the resistance of cancers to cisplatin.

show abstract

“…Several studies have focused on apoptosis-associated proteins in apoptotic cells (18,19). The use of apigenin as an anticancer agent in vitro for the treatment of various cancer cells including prostate, breast, cervical, lung, tongue oral, leukemia and colorectal cancer has increased (20)(21)(22). The evidence of apigenin-induced apoptosis has been demonstrated in a number of cancer cell lines but there is no study on the anticancer action of apigenin on cholangiocarcinoma cell lines.…”

Section: Introductionmentioning

confidence: 99%

Apigenin inhibits growth and induces apoptosis in human cholangiocarcinoma cells

et al. 2017

View full text Add to dashboard Cite

Abstract.A promising nutraceutical, apigenin, was recently revealed to exhibit biological activity in inhibiting several types of cancer. The effects of apigenin on the growth inhibition and apoptosis of the cholangiocarcinoma HuCCA-1 cell line were investigated. Protein alterations subsequent to apigenin treatment were studied using a proteomic approach. The values of 20, 50 and 90% inhibition of cell growth (IC 20 , IC 50 and IC 90 ) were determined by MTT cell viability assay. Apoptotic cell death was detected using two different methods, a flow cytometric analysis (Muse Cell Analyzer) and DNA fragmentation assay. A number of conditions including attached and detached cells were selected to perform two-dimensional gel electrophoresis (2-DE) to study the alterations in the expression levels of treated and untreated proteins and identified by liquid chromatography (LC)/tandem mass spectrometry (MS/MS). The IC 20 , IC 50 and IC 90 values of apigenin after 48 h treatment in HuCCA-1 cells were 25, 75 and 200 µM, respectively, indicating the cytotoxicity of this compound. Apigenin induced cell death in HuCCA-1 cells via apoptosis as detected by flow cytometric analysis and exhibited, as confirmed with DNA fragmentation, characteristics of apoptotic cells. A total of 67 proteins with altered expression were identified from the 2-DE analysis and LC/MS/MS. The cleavage of proteins involved in cytoskeletal, cytokeratin 8, 18 and 19, and high expression of S100-A6 and S100-A11 suggested that apoptosis was induced by apigenin via the caspase-dependent pathway. Notably, two proteins, heterogeneous nuclear ribonucleoprotein H and A2/B1, disappeared completely subsequent to treatment, suggesting the role of apigenin in inducing cell death. The present study indicated that apigenin demonstrates an induction of growth inhibition and apoptosis in cholangiocarcinoma cells and the apoptosis pathway was confirmed by proteomic analysis.

show abstract

Synergistic Effects of Apigenin and Paclitaxel on Apoptosis of Cancer Cells

Cited by 124 publications

References 47 publications

Characteristic Features of Cytotoxic Activity of Flavonoids on Human Cervical Cancer Cells

Characteristic Features of Cytotoxic Activity of Flavonoids on Human Cervical Cancer Cells

Apigenin enhances the cisplatin cytotoxic effect through p53-modulated apoptosis

Apigenin inhibits growth and induces apoptosis in human cholangiocarcinoma cells

Contact Info

Product

Resources

About