Synergistic effects of dexamethasone and quinolones on human-derived tendon cells

Sendzik, Judith; Shakibaei, Mehdi; Schäfer‐Korting, Monika; Lode, H.; Stahlmann, Ralf

doi:10.1016/j.ijantimicag.2009.10.009

Cited by 72 publications

(78 citation statements)

References 47 publications

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“…When incubated with monolayers of human tenocytes, ciprofloxacin significantly decreased type I collagen, beta (1)-integrin receptors, cytoskeletal and signalling proteins and increased matrix metalloproteinases as well as the apoptosis marker activated caspase-3. Effects are intensified at higher concentrations and longer incubation periods (Corps et al, 2005;Sendzik et al, 2005Sendzik et al, , 2010. Similar to the above findings, we observed hyaline degeneration and deformation of parallelism in ciprofloxacin, pefloxacin and ofloxacin treated groups.…”

Section: Discussionsupporting

confidence: 90%

Oral toxicity of pefloxacin, norfloxacin, ofloxacin and ciprofloxacin: comparison of biomechanical and histopathological effects on Achilles tendon in rats

Olcay¹,

Beytemür

Kaleagasioglu

et al. 2011

J. Toxicol. Sci.

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-Four fluoroquinolones (pefloxacin, norfloxacin, ofloxacin and ciprofloxacin) were compared according to their biomechanical and histopathological effects on rat Achilles tendon. Wistar rats were divided into one untreated control and four treatment groups in parallel. Pefloxacin mesylate dihydrate (40 mg/kg), norfloxacin (40 mg/kg), ofloxacin (20 mg/kg) and ciprofloxacin (50 mg/kg) were administered by gavage twice daily for three consecutive weeks. 6 weeks after treatment, the test animals were euthanised and Achilles tendon specimens were collected. A computer monitored tensile testing machine was utilised for biomechanical testing. The mean elastic modulus of the control group was significantly higher than that of the norfloxacin and pefloxacin groups (p < 0.05 and p < 0.01, respectively). The mean yield force (YF) of the control group was significantly higher than those of ciprofloxacin, norfloxacin and pefloxacin groups (p < 0.001, p < 0.05 and p < 0.01, respectively). The mean ultimate tensile force (UTF) of the control group was significantly higher than of the ciprofloxacin, norfloxacin, and pefloxacin groups (p < 0.001, p < 0.05 and p < 0.01, respectively). Hyaline degeneration and fibre disarrangement were observed in the tendons of the ciprofloxacin, pefloxacin, and ofloxacin treated-groups, whereas myxomatous degeneration was observed only in the ciprofloxacin and pefloxacin groups. In conclusion, these findings in our rat model reveal significant deterioration of biomechanical parameters following fluoroquinolone exposure, and indicate significantly higher biomechanical toxicity for ciprofloxacin and pefloxacin.

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Section: Discussionsupporting

confidence: 90%

Oral toxicity of pefloxacin, norfloxacin, ofloxacin and ciprofloxacin: comparison of biomechanical and histopathological effects on Achilles tendon in rats

Olcay¹,

Beytemür

Kaleagasioglu

et al. 2011

J. Toxicol. Sci.

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“…The liver and skeletal muscle were dissected in control and treatment groups, cut into 1 mm 3 tissue blocks and fixed in glutaraldehyde at 4°C. A series of pretreatment procedures, such as poaching, fixing, dehydrating, embedding and sectioning, were performed to prepare tissues for TEM observation according to Sendzik and coworkers (Sendzik et al, 2010).…”

Section: Preparation Of Biological Samples For Transmission Electron mentioning

confidence: 99%

Joint toxicity of fluoroquinolone and tetracycline antibiotics to zebrafish (<i>Danio rerio</i>) based on biochemical biomarkers and histopathological observation

et al. 2017

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-Herein, we report on the joint toxicity of four fluoroquinolones and two tetracyclines (β-diketone antibiotics-DKAs) to zebrafish based on a series of toxicological endpoints and histopathological observations. A positive dose-dependence was observed in DKA-exposure groups with a 72-hpf EC 50 of 130.3 mg/L for hatching rate, 120-hpf LC 50 of 149.8 mg/L, and 120-hpf EC 50 of 135.1 mg/L for malformation rate. When zebrafish at 60 dpf were exposed to a series of DKA concentrations (45, 60 and 90 mg/L) for 7, 14 and 21 days, creatine kinase and AChE activities were significantly induced, and intracellular malondialdehyde increased in all treatments except for the 45 mg/L treatment. The transcription levels of AHRRa from livers were significantly (p < 0.05) up-regulated in all treatments after two months of DKA exposure. CKma expression from skeletal muscle was significantly down-regulated in the 90 mg/L treatment. A remarkable down-regulation of CYP3A65 was observed in the 60 mg/L treatment. DKA exposure resulted in severe tissue damage including mitochondria swelling, reduction of mitochondrial cristae, deepening of mitochondrial cristae bands, and decreasing and even disappearance of the rough endoplasmic reticulum. Total sperm motility was decreased by ca. 30% due to DKA exposure. These results provide important information for toxicity and health risks due to mixed DKA exposure in aquatic environments.

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“…1 This is a degenerative disease with slow healing, and rarely the tendon recovers their original strength and elasticity. 2 Although disease etiology has been considered as multi-factorial, it is known that repetitive mechanical stress is one of the most common mechanisms involved in development of this inflammatory disorder.…”

mentioning

confidence: 99%

Low‐level laser therapy in collagenase‐induced Achilles tendinitis in rats: Analyses of biochemical and biomechanical aspects

Marcos

Leal-Junior

Arnold

et al. 2012

Journal Orthopaedic Research

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NSAIDs are widely prescribed and used over the years to treat tendon injuries despite its well-known long-term side effects. In the last years several animal and human trials have shown that low-level laser therapy (LLLT) presents modulatory effects on inflammatory markers, however the mechanisms involved are not fully understood. The aim of this study was to evaluate the shortterm effects of LLLT or sodium diclofenac treatments on biochemical markers and biomechanical properties of inflamed Achilles tendons. Wistar rats Achilles tendons (n ¼ 6/group) were injected with saline (control) or collagenase at peritendinous area of Achilles tendons. After 1 h animals were treated with two different doses of LLLT (810 nm, 1 and 3 J) at the sites of the injections, or with intramuscular sodium diclofenac. Regarding biochemical analyses, LLLT significantly decreased (p < 0.05) COX-2, TNF-a, MMP-3, MMP-9, and MMP-13 gene expression, as well as prostaglandin E 2 (PGE 2 ) production when compared to collagenase group. Interestingly, diclofenac treatment only decreased PGE 2 levels. Biomechanical properties were preserved in the laser-treated groups when compared to collagenase and diclofenac groups. We conclude that LLLT was able to reduce tendon inflammation and to preserve tendon resistance and elasticity. ß

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Synergistic effects of dexamethasone and quinolones on human-derived tendon cells

Cited by 72 publications

References 47 publications

Oral toxicity of pefloxacin, norfloxacin, ofloxacin and ciprofloxacin: comparison of biomechanical and histopathological effects on Achilles tendon in rats

Oral toxicity of pefloxacin, norfloxacin, ofloxacin and ciprofloxacin: comparison of biomechanical and histopathological effects on Achilles tendon in rats

Joint toxicity of fluoroquinolone and tetracycline antibiotics to zebrafish (<i>Danio rerio</i>) based on biochemical biomarkers and histopathological observation

Low‐level laser therapy in collagenase‐induced Achilles tendinitis in rats: Analyses of biochemical and biomechanical aspects

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