Synergistic effects of endolysin Lysqdvp001 and ε-poly-lysine in controlling Vibrio parahaemolyticus and its biofilms

Ning, Houqi; Lin, Hong; Wang, Jingxue

doi:10.1016/j.ijfoodmicro.2021.109112

Cited by 45 publications

(19 citation statements)

References 46 publications

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“…The effects of co-expressing X2-Lys and X2-Hol on cell membranes was further measured using β-galactosidase release assays, as described above. Similar to the result from pET28a, no change in color was observed using the empty pETDuet-1 plasmid, indicating cell membranes remained intact and that the β-galactosidase synthesized in the cell is unable to cross the cell membrane [51,52]. Color change (indicating release of the enzyme) was observed in bacteria expressing either X2-Lys or X2-Hol alone after 24 h of induction while bacteria expressing both X2-Lys and X2-Hol showed a much stronger intensity.…”

Section: Effects Of Co-expression Of X2-hol and X2-lys On Cellssupporting

confidence: 71%

The Holin-Endolysin Lysis System of the OP2-Like Phage X2 Infecting Xanthomonas oryzae pv. oryzae

Zhang

et al. 2021

Viruses

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Most endolysins of dsDNA phages are exported by a holin-dependent mechanism, while in some cases endolysins are exported via a holin-independent mechanism. However, it is still unclear whether the same endolysins can be exported by both holin-dependent and holin-independent mechanisms. This study investigated the lysis system of OP2-like phage X2 infecting Xanthomonas oryzae pv. oryzae, causing devastating bacterial leaf blight disease in rice. Based on bioinformatics and protein biochemistry methods, we show that phage X2 employs the classic "holin-endolysin" lysis system. The endolysin acts on the cell envelope and exhibits antibacterial effects in vitro, while the holin facilitates the release of the protein into the periplasm. We also characterized the role of the transmembrane domain (TMD) in the translocation of the endolysin across the inner membrane. We found that the TMD facilitated the translocation of the endolysin via the Sec secretion system. The holin increases the efficiency of protein release, leading to faster and more efficient lysis. Interestingly, in E. coli, the expression of either holin or endolysin with TMDs resulted in the formation of long rod shaped cells. We conclude that the TMD of X2-Lys plays a dual role: One is the transmembrane transport while the other is the inhibition of cell division, resulting in larger cells and thus in a higher number of released viruses per cell.

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Section: Effects Of Co-expression Of X2-hol and X2-lys On Cellssupporting

confidence: 71%

The Holin-Endolysin Lysis System of the OP2-Like Phage X2 Infecting Xanthomonas oryzae pv. oryzae

Zhang

et al. 2021

Viruses

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“…Expectedly, the results in Figures c and b revealed a significant decrease in the Cl + content from 757–1057 to 152–276 ppm. This phenomenon may have resulted from the partial destruction of LPS, promoting surface charge neutralization and interrupting membrane stability and integrity . Taken together, our data confirmed AH@PBAT/PBF-Cl film interaction with the negatively charged bacterial membrane via electrostatic attraction, sticking to the cells and disrupting their fundamental physiological functions.…”

Section: Resultssupporting

confidence: 64%

Super Antibacterial Capacity and Cell Envelope-Disruptive Mechanism of Ultrasonically Grafted N-Halamine PBAT/PBF Films against Escherichia coli

Guo

Zhang

Ismail

et al. 2023

ACS Appl. Mater. Interfaces

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Antibacterial materials are urgently needed to combat bacterial contamination, growth, or attachment on contact surfaces, as bacterial infections remain a public health crisis worldwide. Here, a novel ultrasound-assisted method is utilized for the first time to fabricate oxidative chlorine-loaded AH@PBAT/ PBF-Cl films with more superior grafting efficiency and rechargeable antibacterial effect than those from conventional techniques. The films remarkably inactivate 99.9999% Escherichia coli and Staphylococcus aureus cells, inducing noticeable cell deformations and mechanical instability. The specific antibacterial mechanism against E. coli used as a model organism is unveiled using several cell envelope structural and functional analyses combined with proteomics, peptidoglycomics, and fluorescence probe techniques. Film treatment partially neutralizes the bacterial surface charge, induces oxidative stress and cytoskeleton deformity, alters membrane properties, and disrupts the expression of key proteins involved in the synthesis and transport of the lipopolysaccharide and peptidoglycan, indicating the cell envelope as the primary target. The films specifically target lipopolysaccharides, resulting in structural impairment of the polysaccharide and lipid A components, and inhibit peptidoglycan precursor synthesis. Together, these lead to metabolic disorders, membrane dysfunction, structural collapse, and eventual death. Given the films' antibacterial effects via the disruption of key cell envelope components, they can potentially combat a wide range of bacteria. These findings lay a theoretical basis for developing efficient antibacterial materials for food safety or biomedical applications.

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“…ε-PL combined with chitooligosaccharide (COS; 200 µg/mL ε-PL + 400 µg/mL COS) displayed stronger inhibitory activity (90.22%) against B. cinerea compared with treatment using either agent alone [ 16 ]. In addition, combinations of ε-PL with other compounds, such as endolysin, chitosan, and nisin, demonstrated synergistic antibacterial effects against different pathogens [ 6 , 29 , 30 , 31 ]. The S. albulus CK-15 secondary metabolite wuyiencin is used widely in agriculture to control fungal diseases, including tomato gray mold, strawberry powdery mildew, and cucumber downy mildew [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of ε-Poly-L-Lysine Combined with Wuyiencin as a Bio-Fungicide against Botryris cinerea

Lü

et al. 2022

Microorganisms

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This study mainly evaluated the broad-spectrum fungicidal activity of ε-poly L lysine (ε-PL) against 12 pathogenic fungi. We further demonstrated synergistic antifungal activity of ε-PL combined with wuyiencin against Botryris cinerea. The combined bio-fungicide achieved an inhibition rate of 100% for mycelial growth using ε-PL at 500 μg/mL + wuyiencin at 50 μg/mL and for spore germination using ε-PL at 200 μg/mL + wuyiencin at 80 μg/mL in vitro. This synergistic spore and mycelia-damaging effect of the combination was confirmed using scanning electron microscopy. In vivo assays with combined bio-fungicide (1500 μg/mL ε-PL + 60 μg/mL wuyiencin) on detached leaves showed depressed growth and development of the spores of B. cinerea. The synergistic effect was further tested in combinations of ε-PL with wuyiencin by measuring the fractional inhibition concentration index (FICI) value below 0.5. Moreover, ε-PL and wuyiencin inoculation before B. cinerea infection significantly increased the superoxide dismutase, peroxidase, catalase, and phenylalanine ammonia-lyase activities, which suggested their involvement in tomato defense responses to disease to minimize damage to B. cinerea. These findings revealed that a combined bio-fungicide comprising ε-PL and wuyiencin had a good prospect for controlling plant fungal disease.

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Synergistic effects of endolysin Lysqdvp001 and ε-poly-lysine in controlling Vibrio parahaemolyticus and its biofilms

Cited by 45 publications

References 46 publications

The Holin-Endolysin Lysis System of the OP2-Like Phage X2 Infecting Xanthomonas oryzae pv. oryzae

The Holin-Endolysin Lysis System of the OP2-Like Phage X2 Infecting Xanthomonas oryzae pv. oryzae

Super Antibacterial Capacity and Cell Envelope-Disruptive Mechanism of Ultrasonically Grafted N-Halamine PBAT/PBF Films against Escherichia coli

Effects of ε-Poly-L-Lysine Combined with Wuyiencin as a Bio-Fungicide against Botryris cinerea

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