Synergistic effects of green tea extract and paclitaxel in the induction of mitochondrial apoptosis in ovarian cancer cell lines

Panji, Mohammad; Behmard, Vahideh; Zare, Zahra; Malekpour, Monireh; Nejadbiglari, Hasan; Yavari, Saeede; dizaj, Tina Nayerpour; Safaeian, Azadeh; Bakhshi, Ali; Abazari, Omid; Abbasi, Mojtaba; Khanicheragh, Parisa; Shabanzadeh, Maryam

doi:10.1016/j.gene.2021.145638

Cited by 27 publications

(12 citation statements)

References 36 publications

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“…Many studies have demonstrated that increased Akt signaling pathway activation leads to a lower apoptosis rate in multiple cancer types via phosphorylation and the inactivation of pro-apoptotic mediators such as the Bad protein [ 47 ]. The inhibition of the Akt pathway was associated with the activation of the mitochondrial apoptotic pathway characterized by an important reduction in the anti-apoptotic BCL-2 protein and a notable increase in the Bad levels, Cyt-c, cleaved-caspases-3 and -9, and Bax [ 48 ].…”

Section: Polyphenols As Anticancer Agentsmentioning

confidence: 99%

Combination Therapy Using Polyphenols: An Efficient Way to Improve Antitumoral Activity and Reduce Resistance

Vladu

Ficai

Ene

et al. 2022

IJMS

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Polyphenols represent a structural class of mainly natural organic chemicals that contain multiple phenol structural units. The beneficial properties of polyphenols have been extensively studied for their antitumor, anti-inflammatory, and antibacterial effects, but nowadays, their medical applications are starting to be extended to many other applications due to their prebiotic role and their impact on the microbiota. This review focused on the use of polyphenols in cancer treatment. Their antineoplastic effects have been demonstrated in various studies when they were tested on numerous cancer lines and some in in vivo models. A431 and SCC13 human skin cancer cell lines treated with EGCG presented a reduced cell viability and enhanced cell death due to the inactivation of β-catenin signaling. Additionally, resveratrol showed a great potential against breast cancer mainly due to its ability to exert both anti-estrogenic and estrogenic effects (based on the concentration) and because it has a high affinity for estrogen receptors ERα and Erβ. Polyphenols can be combined with different classical cytostatic agents to enhance their therapeutic effects on cancer cells and to also protect healthy cells from the aggressiveness of antitumor drugs due to their anti-inflammatory properties. For instance, curcumin has been reported to reduce the gastrointestinal toxicity associated with chemotherapy. In the case of 5-FU-induced, it reduced the gastrointestinal toxicity by increasing the intestinal permeability and inhibiting mucosal damage. Co-administration of EGCG and doxorubicin induced the death of liver cancer cells. EGCG has the ability to inhibit autophagic activity and stop hepatoma Hep3B cell proliferation This symbiotic approach is well-known in medical practice including in multiple chemotherapy.

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Section: Polyphenols As Anticancer Agentsmentioning

confidence: 99%

Combination Therapy Using Polyphenols: An Efficient Way to Improve Antitumoral Activity and Reduce Resistance

Vladu

Ficai

Ene

et al. 2022

IJMS

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“…Green tea targeted CD44 as a safe nanomedicine treatment, thus inhibiting tumor progression and recurrence ( Bae et al, 2017 ). It had been demonstrated that EGCG efficacies via targeting a number of genes including proliferative related genes JUN, FADD, NFKB1, HIF1α, and matrix metalloproteinases (MMPs) ( Xinqiang et al, 2017 ), apoptotic related genes Bcl-2, Bax, caspases ( Panji et al, 2021a ) as well as downregulated PTEN/AKT/mTOR pathway to inhibit proliferation in vitro and in vivo ( Qin et al, 2020 ). EGCG promoted DNA damage response, reduced DNA synthesis, and enhanced oxidative stress to induce apoptosis in cells ( Mazumder et al, 2012 ) ( Rao and Pagidas, 2010 ) ( Chan et al, 2006 ).…”

Section: Green Teamentioning

confidence: 99%

“…Future studies can also focus on combining EGCG with other anticancer drugs to achieve a synergistic enhancement of the anti-cancer effects ( Suganuma et al, 2011 ; Fujiki et al, 2015 ). In ovarian cancer, green tea with Paclitaxel or other nutrients as combination strategy synergistically inhibited cancer cells ( Xinqiang et al, 2017 ; Panji et al, 2021a ). Meanwhile, EGCG in combination with Cruciferous vegetables or Sulforaphane were shown to overcome Cisplatin or Paclitaxel chemotherapy resistance in ovarian cancer ( Mazumder et al, 2012 ; Chen et al, 2013b ; a ).…”

Section: Current Limitation and Future Perspectives Of Egcg Developmentmentioning

confidence: 99%

“…In breast cancer, EGCG demonstrated synergistic effects with Dexamethasone, U021, Tamoxifen, Quercetin or Paclitaxel ( Guo et al, 2006 ; Sartippour et al, 2006 ; Li et al, 2009 ; Luo et al, 2010 ), thus enhanced cancer cell death. As discussed, tea polyphenol was proposed to be used with other chemotherapies to synergistically advance the therapeutic efficiency of autophagy modulating effect, such as with Docetaxel or Bortezomib in prostate cancer ( Modernelli et al, 2015 ; Wang Q. et al, 2018 ), with Paclitaxel in ovarian cancer ( Panji et al, 2021a ), or with Doxorubicin in cervical cancer ( Chen et al, 2020 ).…”

Section: Current Limitation and Future Perspectives Of Egcg Developmentmentioning

confidence: 99%

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Green Tea Epigallocatechin-3-Gallate Regulates Autophagy in Male and Female Reproductive Cancer

Hung

Chen

et al. 2022

Front. Pharmacol.

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With a rich abundance of natural polyphenols, green tea has become one of the most popular and healthiest nonalcoholic beverages being consumed worldwide. Epigallocatechin-3-gallate (EGCG) is the predominant catechin found in green tea, which has been shown to promote numerous health benefits, including metabolic regulation, antioxidant, anti-inflammatory, and anticancer. Clinical studies have also shown the inhibitory effects of EGCG on cancers of the male and female reproductive system, including ovarian, cervical, endometrial, breast, testicular, and prostate cancers. Autophagy is a natural, self-degradation process that serves important functions in both tumor suppression and tumor cell survival. Naturally derived products have the potential to be an effective and safe alternative in balancing autophagy and maintaining homeostasis during tumor development. Although EGCG has been shown to play a critical role in the suppression of multiple cancers, its role as autophagy modulator in cancers of the male and female reproductive system remains to be fully discussed. Herein, we aim to provide an overview of the current knowledge of EGCG in targeting autophagy and its related signaling mechanism in reproductive cancers. Effects of EGCG on regulating autophagy toward reproductive cancers as a single therapy or cotreatment with other chemotherapies will be reviewed and compared. Additionally, the underlying mechanisms and crosstalk of EGCG between autophagy and other cellular processes, such as reactive oxidative stress, ER stress, angiogenesis, and apoptosis, will be summarized. The present review will help to shed light on the significance of green tea as a potential therapeutic treatment for reproductive cancers through regulating autophagy.

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“…Today, natural compounds and their derivatives play an important role in the clinical treatment of various types of cancers, making up about 63% of commercially available drugs (19,22,23). Some compounds such as vincristine, etoposide, and paclitaxel are examples of plant-derived anticancer drugs, and compounds such as actinomycin D, mitomycin C, bleomycin, doxorubicin, and L-asparaginase are compounds derived from microorganisms (23)(24)(25). About 23,000 secondary metabolites have been discovered from microorganisms, and approximately about 10,000 species (about 45%) have been extracted from actinomycetes (26,27).…”

Section: Introductionmentioning

confidence: 99%

Secondary Metabolites of Soil Actinomycetes, UTMC 676 and UTMC 919, Induces Apoptosis in Human Non‑Small-Cell Lung Cancer Cell Line

Gardeshi

Norbakhsh²,

Dalvand³

et al. 2022

Stud Med Sci

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Background & Aims: Bacterial metabolites are extremely rich resources for discovering new compounds with different biological activities. Metabolites of actinomycetes have significant potential for the production of anticancer compounds. The purpose of this research is to investigate the effects of two secondary metabolites of soil actinomycetes, UTMC 676 and UTMC 919, on apoptosis induction and their related genes in the human non-small cell lung carcinoma cell line, A549. Materials & Methods:The crude extracts of UTMC 676 and UTMC 919 were prepared from the collection of biological compounds of Tehran University. After cell treatment with UTMC 676 and UTMC 919, cell cytotoxicity, apoptosis, and mRNA expression were measured using MTT, flow cytometry, and q-RT-PCR methods. Doxorubicin was utilized as a positive control. Results:The MTT results showed induction of cytotoxicity by UTMC 676, UTMC 919, and doxorubicin in A549 cells in a concentration-dependent manner. After 48 hours of treatment, both UTMC 676 and UTMC 919 induced apoptosis in the A549 cell line. However, the apoptotic effect of UTMC 676 was more than doxorubicin. The q-RT-PCR data exhibited that the expression of apoptosis-related genes was enhanced in the treated group compared to the untreated group. Conclusion:These results suggest that the crude extract of UTMC 676 was able to induce apoptosis in A549 cells and could be a very promising source having therapeutic potential against lung cancer cell lines.

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Synergistic effects of green tea extract and paclitaxel in the induction of mitochondrial apoptosis in ovarian cancer cell lines

Cited by 27 publications

References 36 publications

Combination Therapy Using Polyphenols: An Efficient Way to Improve Antitumoral Activity and Reduce Resistance

Combination Therapy Using Polyphenols: An Efficient Way to Improve Antitumoral Activity and Reduce Resistance

Green Tea Epigallocatechin-3-Gallate Regulates Autophagy in Male and Female Reproductive Cancer

Secondary Metabolites of Soil Actinomycetes, UTMC 676 and UTMC 919, Induces Apoptosis in Human Non‑Small-Cell Lung Cancer Cell Line

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