Synergistic effects of protein tyrosine kinase inhibitor genistein with camptothecins against three cell lines in vitro

Papazisis, Konstantinos; Kalemi, Theodora G.; Zambouli, Dimitra; Geromichalos, George D.; Lambropoulos, Alexandros; Kotsis, A.; Boutis, L.; Kortsaris, Alexandros

doi:10.1016/j.canlet.2005.03.022

Cited by 33 publications

(24 citation statements)

References 35 publications

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“…32,[38][39][40] Additionally, genistein has also been shown to synergize with cisplatin in exerting an antiproliferative effect in a variety of other tumor types such as human malignant melanoma, medulloblastoma and ovarian cancer cells in vitro. [41][42][43][44] Furthermore, previous studies in animal models have shown that genistein potentiates the effect of other therapies, including radio-, immuno-and chemotherapy.…”

Section: Discussionmentioning

confidence: 99%

Retracted: In vitro and in vivo molecular evidence of genistein action in augmenting the efficacy of cisplatin in pancreatic cancer

Banerjee

Zhang

Wang

et al. 2006

Intl Journal of Cancer

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We recently reported the potential of genistein in augmenting gemcitabine-induced killing of pancreatic cancer (Banerjee, S. et al., Cancer Research 2005;65:9064-72). Since cis-diaminedichloroplatinum (II) (cisplatin) is widely used against solid tumors, we further investigated whether genistein pretreatment could be used as a novel strategy for cisplatin-induced killing of pancreatic cancer cells in vitro and enhanced antitumor activity in vivo. Our in vitro results showed that pretreatment of cells with genistein followed by cisplatin resulted in significant loss of cell viability and potentiated apoptosis irrespective of the metastatic ability of cells. Mechanistically, genistein augmented cisplatin induced killing by down regulating transcription factor-NF-jB and anti-apoptotic Akt expression. NF-jB was found upregulated when pancreatic cancer cells were exposed to cisplatin, suggesting the potential mechanism of acquired chemo-resistance. In addition, we also showed, for the first time, that genistein in combination with cisplatin is more effective antitumor agent in our orthotopic tumor model. But most importantly, our data also showed that a specific target, such as NF-jB, was inactivated in animal tumors treated with genistein and cisplatin. Immunohistochemical data showed reduced staining for phospho-p65, Bcl-xL and MMP-9 in treated tumors compared to control tumors, but the lowest activity was seen in the combination group. These results provide strong molecular in vivo evidence in support of our hypothesis that inactivation of the NF-jB signaling pathway by genistein results in the chemo-sensitization of pancreatic tumors to cisplatin, which is likely to be an important and novel strategy for the treatment of pancreatic cancer. ' 2006 Wiley-Liss, Inc.

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Section: Discussionmentioning

confidence: 99%

Retracted: In vitro and in vivo molecular evidence of genistein action in augmenting the efficacy of cisplatin in pancreatic cancer

Banerjee

Zhang

Wang

et al. 2006

Intl Journal of Cancer

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“…These compounds have shown to reduce the risk of cardiovascular disease (Jackman et al 2007) and promote the inhibition of cancer cell growth (Davis et al 2008;Kao et al 2007a;Kohen et al 2007;Sarkar and Li 2003). Furthermore, isoflavones play an important role in the prevention of several diseases, including osteoporosis, and menopausal symptoms (Coxam 2008;Dijsselbloem et al 2004;Ma et al 2008;Phrakonkham et al 2007) since they act as anti-estrogens (Okamoto et al 2006;Zhang et al 2007a) and tyrosine protein kinase inhibitors (Papazisis et al 2006). The effects of these flavonoid substances are strongly influenced by the chemical structure of the naturally occurring compound, for example, hydroxyl substitution of the B-and C-rings, but not the A-ring is essential for antioxidant activity (see Fig.…”

Section: Introductionmentioning

confidence: 94%

Content and Profile of Isoflavones in Soy-Based Foods as a Function of the Production Process

Villares

Rostagno

García–Lafuente

et al. 2010

Food Bioprocess Technol

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Soy has been traditionally incorporated in diet as processed foods, such as soymilk, tofu, miso, tempeh, etc., and the consumption is commonly associated with a reduction of the development of chronic diseases due to their antioxidant, anti-inflammatory, and anti-allergic properties, among others. Many of the health benefits of soy have been attributed to isoflavones. They comprise a group of naturally occurring flavonoids consisting of heterocyclic phenols. Soy contains three types of isoflavones in four chemical forms: the aglycones daidzein, genistein, and glycitein; the β-glucosides daidzin, genistin, and glycitin; their 6″-O-malonyl-β-glucosides (6OMalGlc); and their 6″-O-acetyl-β-glucosides (6OAcGlc) conjugates. Industrial processing methods of soy-based food products commonly lead to the loss of isoflavones through removal of undesirable fractions. On the other hand, isoflavones can be transformed into different conjugates, which may have significant effects on the food texture and on the bioavailability and pharmacokinetics of the isoflavones. This article reviews the effect of a number of soybean processing treatments on the isoflavone content and profile. The preparation and manufacturing of different soy-based food and food ingredients, fermented and non-fermented, has been analyzed in terms of content and distribution of the three major isoflavone derivatives, daidzein, genistein, and glycitein, and their respective conjugates.

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“…This isoflavone can reduce the viability of various human cervical cancer cell lines differing from one another in their HPV status and origin (see Table 2). Such anticancer action might work through numerous mechanisms, including arresting the cell cycle, initiating apoptotic changes and suppressing the invasive potential of tumor cells (Wang et al, 2001;Papazisis et al, 2006;Kim et al, 2009b;Zhou et al, 2009;Jha et al, 2010;Hussain et al, 2012;Dayuthapani et al, 2013).…”

Section: Isoflavonesmentioning

confidence: 99%

Characteristic Features of Cytotoxic Activity of Flavonoids on Human Cervical Cancer Cells

Sak¹

2014

Asian Pacific Journal of Cancer Prevention

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Cervical cancer is the most common gynecologic malignancy worldwide and development of new therapeutic strategies and anticancer agents is an urgent priority. Plants have remained an important source in the search for novel cytotoxic compounds and several polyphenolic flavonoids possess antitumor properties. In this review article, data about potential anticarcinogenic activity of common natural flavonoids on various human cervical cancer cell lines are compiled and analyzed showing perspectives for the use of these secondary metabolites in the treatment of cervical carcinoma as well as in the development of novel chemotherapeutic drugs. Such anticancer effects of flavonoids seem to differentially depend on the cellular type and origin of cervical carcinoma creating possibilities for specific targeting in the future. Besides the cytotoxic activity per se, several flavonoids can also contribute to the increase in efficacy of conventional therapies rendering tumor cells more sensitive to standard chemotherapeutics and irradiation. Although the current knowledge is still rather scarce and further studies are certainly needed, it is clear that natural flavonoids may have a great potential to benefit cervical cancer patients.

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Synergistic effects of protein tyrosine kinase inhibitor genistein with camptothecins against three cell lines in vitro

Cited by 33 publications

References 35 publications

Retracted: In vitro and in vivo molecular evidence of genistein action in augmenting the efficacy of cisplatin in pancreatic cancer

Retracted: In vitro and in vivo molecular evidence of genistein action in augmenting the efficacy of cisplatin in pancreatic cancer

Content and Profile of Isoflavones in Soy-Based Foods as a Function of the Production Process

Characteristic Features of Cytotoxic Activity of Flavonoids on Human Cervical Cancer Cells

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