Synergistic effects of resveratrol and melatonin on in vitro maturation of porcine oocytes and subsequent embryo development

Lee, Sanghoon; Jin, Jun-Xue; Taweechaipaisankul, Anukul; Kim, Geon A; Lee, Byeong Chun

doi:10.1016/j.theriogenology.2018.03.040

Cited by 41 publications

(30 citation statements)

References 44 publications

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“…The thermal cycler condition used for the amplification of all genes began with an initial denaturation at 95°C for 10 min, followed by 40 PCR cycles of 95°C for 15 s, 60 °C for 1 min and 72°C for 1 min. Each transcript sample was quantified in biological and technical triplicates by using the

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method (Lee, Jin, Taweechaipaisankul, Kim, & Lee, ), and the mRNA levels of all genes were normalized to the housekeeping gene Glyceraldehyde‐3‐phosphate dehydrogenase ( GAPDH ). The average expression level of each gene from the control group was set as 1 for ease of comparison.…”

Section: Methodsmentioning

confidence: 99%

Enhancement of epigenetic reprogramming status of porcine cloned embryos with zebularine, a DNA methyltransferase inhibitor

Taweechaipaisankul

Jin

Lee

et al. 2019

Molecular Reproduction Devel

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Aberrant epigenetic reprogramming is known to be a major cause of inefficient somatic cell nuclear transfer (SCNT) in pigs, and use of epigenetic modification agents, such as DNA methyltransferase inhibitors (DNMTis), is a promising approach for enhancing SCNT efficacy. Here, we attempted to find the optimal condition of zebularine (Zb), a DNMTi, treatment on porcine SCNT embryos during in vitro culture (IVC). As results, treatment with 5 nM Zb for 24 hr showed the highest rate of embryo development to blastocyst compared to other groups (p < .05). Also, the relative intensities of global DNA methylation levels of anti‐5‐methylcytosine in pseudo‐pronuclear (PNC), 2‐cell and 4‐cell stages were significantly lower in the Zb‐treated group (p < .05), however, changes in methylation levels of centromeric satellite repeat were noted only in PNC and blastocyst stages. In addition, significant positive alterations in the relative expression of genes related to pluripotency (OCT4 and SOX2), histone acetylation (HAT1, HDAC1, HDAC2, and HDAC3) and DNA methylation (DNMT1 and DNMT3a) were observed compared to the control (p < .05). In conclusion, we found that Zb could modify DNA methylation levels in the early stages of porcine SCNT embryos and promote their developmental competence.

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Section: Methodsmentioning

confidence: 99%

Enhancement of epigenetic reprogramming status of porcine cloned embryos with zebularine, a DNA methyltransferase inhibitor

Taweechaipaisankul

Jin

Lee

et al. 2019

Molecular Reproduction Devel

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“…Medium with 10 −5 M melatonin enhances blastocyst rates of in vitro PA embryos via melatonin receptors, and luzindole can be used as a melatonin receptor antagonist [8]. Resveratrol and melatonin have synergistic effects on improving oocyte nuclear IVM, total cell numbers of PA blastocysts, and development of porcine nuclear transfer embryos [9].…”

Section: Introductionmentioning

confidence: 99%

Effect of Melatonin on the In Vitro Maturation of Porcine Oocytes, Development of Parthenogenetically Activated Embryos, and Expression of Genes Related to the Oocyte Developmental Capability

Yang

Wang

Cui

et al. 2020

Animals

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Melatonin treatment can improve quality and in vitro development of porcine oocytes, but the mechanism of improving quality and developmental competence is not fully understood. In this study, porcine cumulus–oocyte complexes were cultured in TCM199 medium with non-treated (control), 10−5 M luzindole (melatonin receptor antagonist), 10−5 M melatonin, and melatonin + luzindole during in vitro maturation, and parthenogenetically activated (PA) embryos were treated with nothing (control), or 10−5 M melatonin. Cumulus oophorus expansion, oocyte survival rate, first polar body extrusion rate, mitochondrial distribution, and intracellular levels of reactive oxygen species (ROS) and glutathione of oocytes, and cleavage rate and blastocyst rate of the PA embryos were assessed. In addition, expression of growth differentiation factor 9 (GDF9), tumor protein p53 (P53), BCL2 associated X protein (BAX), catalase (CAT), and bone morphogenetic protein 15 (BMP15) were analyzed by real-time quantitative PCR. The results revealed that melatonin treatment not only improved the first polar body extrusion rate and cumulus expansion of oocytes via melatonin receptors, but also enhanced the rates of cleavage and blastocyst formation of PA embryos. Additionally, melatonin treatment significantly increased intraooplasmic level of glutathione independently of melatonin receptors. Furthermore, melatonin supplementation not only significantly enhanced mitochondrial distribution and relative abundances of BMP15 and CAT mRNA, but also decreased intracellular level of ROS and relative abundances of P53 and BAX mRNA of the oocytes. In conclusion, melatonin enhanced the quality and in vitro development of porcine oocytes, which may be related to antioxidant and anti-apoptotic mechanisms.

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“…Phytomelatonin is a well-known product used in phytomedicine for its antioxidant properties [ 53 ]. In this context, Lee et al [ 54 ] investigated the synergistic properties of melatonin and resveratrol to ameliorate porcine IVM of oocytes. These authors found that the association of the two compounds in the medium of cumulus-oocyte complexes undergoing IVM supported a synergistic increase in oocyte nuclear maturation and total cell numbers of parthenogenetic activated blastocysts, and improved the development of somatic cell nuclear transfer embryos [ 54 ].…”

Section: Resveratrol Effects On Femalesmentioning

confidence: 99%

The Role of Resveratrol in Mammalian Reproduction

et al. 2020

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Resveratrol is one of the most investigated natural polyphenolic compounds and is contained in more than 70 types of plants and in red wine. The widespread interest in this polyphenol derives from its antioxidant, anti-inflammatory and anti-aging properties. Several studies have established that resveratrol regulates animal reproduction. However, the mechanisms of action and the potential therapeutic effects are still unclear. This review aims to clarify the role of resveratrol in male and female reproductive functions, with a focus on animals of veterinary interest. In females, resveratrol has been considered as a phytoestrogen due to its capacity to modulate ovarian function and steroidogenesis via sirtuins, SIRT1 in particular. Resveratrol has also been used to enhance aged oocyte quality and as a gametes cryo-protectant with mainly antioxidant and anti-apoptotic effects. In males, resveratrol enhances testes function and spermatogenesis through activation of the AMPK pathway. Furthermore, resveratrol has been supplemented to semen extenders, improving the preservation of sperm quality. In conclusion, resveratrol has potentially beneficial effects for ameliorating ovarian and testes function.

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Synergistic effects of resveratrol and melatonin on in vitro maturation of porcine oocytes and subsequent embryo development

Cited by 41 publications

References 44 publications

Enhancement of epigenetic reprogramming status of porcine cloned embryos with zebularine, a DNA methyltransferase inhibitor

Enhancement of epigenetic reprogramming status of porcine cloned embryos with zebularine, a DNA methyltransferase inhibitor

Effect of Melatonin on the In Vitro Maturation of Porcine Oocytes, Development of Parthenogenetically Activated Embryos, and Expression of Genes Related to the Oocyte Developmental Capability

The Role of Resveratrol in Mammalian Reproduction

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