Synergistic effects of salicylic acid and light stress on bioactive metabolites in basil callus cultures

Nazir, Saher; Jan, Hasnain; Zaman, Gouhar; Ahmed, Nisar; Drouet, Samantha; Hano, Christophe; Abbasi, Bilal Haider

doi:10.1016/j.bcab.2021.102176

Cited by 13 publications

(5 citation statements)

References 67 publications

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“…The role of copper in plant growth was observed in other medicinal plants, such as Ocimum basilicum L. cultured in vitro in medium containing 25 µM CuSO 4 , where treated plants had more leaves, were more vigorous and showed a 17% reduction in shoot oxidation(Trettel et al 2018). Callus cultures of O. basilicum had higher biomass accumulation when subjected to abiotic elicitation (10 mg L − 1 copper), in addition to a higher production of secondary metabolites, such as antioxidants, avonoids and phenolics(Nazir et al 2021). CuSO 4 (2 mg L − 1 ) positively in uenced fresh and dry biomass weights, total phenolic accumulation, and antioxidant activity in Artemisia annua L. calli(Zarad et al.…”

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confidence: 85%

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Biochemical responses and volatile compounds in a peppermint chemotype grown in a controlled environment

Calgaro

Melisinas

Gonçalves

et al. 2023

Preprint

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Peppermint is a medicinal plant with great economic importance for its protective effects against biotic and abiotic factors. This study aimed to assess the vegetative growth, biochemistry and volatile production of peppermint plants under elicitation. Nodal segments were inoculated in flasks containing Murashige and Skoog medium with one of four treatments (50 µM salicylic acid, 200 mg L− 1 chitosan, 25 µM copper sulphate, and control) and maintained for 90 days in a controlled environment. Copper treatment increased shoot growth by 43% and 68% compared with salicylic acid and chitosan, respectively. Furthermore, copper elicitation reduced the oxidation rate to only 13% and produced plants with better architecture. Salicylic acid and chitosan treatments increased the total phenolic content by 38% and 40%, respectively, compared with the control. The ferric reducing assay showed that salicylic acid and chitosan treatments increased the plant’s antioxidant activity by 82% and 96%, respectively, compared with the control. However, β-carotene, flavonoids and anthocyanins decreased with these treatments. Phenylalanine ammonia-lyase activity increased by 63% and 54% in shoots elicited with salicylic acid and chitosan, respectively. Elicitor treatment increased the number of volatile compounds detected (control = 29, salicylic acid = 32, copper = 37, chitosan = 38). Elicitation promoted significant changes in plant metabolism and chemical composition, evidenced mainly by differences in the levels of 2-hydroxy-3-(3-methyl-2-butenyl)-3-cyclopenten-1-one, d-limonene, eucalyptol, caryophyllene and l-alanine ethylamide. Monoterpene hydrocarbons were the major class in control shoots, whereas oxygenated monoterpenes were the major compounds in elicited shoots.

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confidence: 85%

“…UV-B light and melatonin (elicitors) increased rosmarinic, caffeic and chicoric acid levels, as well as the antioxidant capacity, in calli of O. basilicum var. purpurascens(Nazir et al 2021). In the current study, we also analysed the pro le of phenolic compounds that act as pigments in plants ( avonoids and anthocyanins).…”

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confidence: 99%

Biochemical responses and volatile compounds in a peppermint chemotype grown in a controlled environment

Calgaro

Melisinas

Gonçalves

et al. 2023

Preprint

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“…Like the AgNPs, the SA-mediated elicitation also improved callus biomass and longevity. SA treatment has been shown to improve callus traits in in vitro cultures [43]. SA can induce various physiological and biochemical fluctuations in plants, including an increased synthesis of secondary metabolites and antioxidants, enhanced defense responses, and improved tolerance to stresses.…”

Section: Agnp and Sa Application Improved Callus Texture Color Compac...mentioning

confidence: 99%

Enhancing Bioactive Metabolite Production in Aerva sanguinolenta Callus Cultures through Silver Nanoparticle and Salicylic Acid Elicitation

et al. 2023

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Callus elicitation is advantageous for metabolite production due to its ability to increase yield, provide controllable conditions, and allow for genetic manipulation, offering a sustainable and scalable alternative to traditional plant-based extraction methods for the production of bioactive substances. In this research, in vitro callus cultures (CCs) of the wild medicinal plant Aerva sanguinolenta were used to evaluate the efficacy of various elicitation regimes of silver nanoparticles (AgNPs) and salicylic acid (SA) to evoke an increased production of secondary metabolites, such as aervine and antioxidant metabolites. Three concentrations of SA (i.e., 20, 50, and 100 µM) and three concentrations of AgNPs (i.e., 30, 60, and 90 µg/L) were used on shoot explant cultures using MS (Murashige and Skoog) media. All the SA and AgNP elicitation treatments significantly increased the production of antioxidant metabolites, total phenolic contents (TPCs), and total flavonoid contents (TFCs) compared to the control treatment experiments. The contents of aervine were increased significantly upon elicitation compared to the control trial. Furthermore, the antioxidant potential of the test extract was enhanced compared to the control treatment. Comparatively, the AgNPs were more beneficial as elicitors than the SA treatments. The elicitation treatments with about 90 µg/L AgNPs and 100 µM SA were the best among all elicitation regimes. Callus elicitation with SA and AgNPs can stimulate increased metabolite production and be used as a sustainable practice in the welfare and service industries for drug development and drug discovery.

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“…In the last decade, consistent efforts have been made in basil tissue culture and this is largely attributed to the high content of phenolic compounds, in particular phenolic acids, and flavonoids (Jakovljević et al, 2022). Previous reports highlighted the CC and CS culture as a promising strategy for the ongoing and sustainable synthesis of basil high‐value antioxidant constituents (Wongsen et al, 2015; Nazir et al, 2019; Açıkgöz 2020; Berim and Gang 2020; Açıkgöz 2021; Nazir et al, 2021; Mehring et al, 2022). However, these studies also showed that the quantity of high‐value antioxidant constituents is dependent on cultivation conditions, applied growth regulators, as well as the genotype used.…”

Section: Introductionmentioning

confidence: 99%

Untargeted metabolomic in basil cell cultures – a case study of Ocimum basilicum L. var. minimum Alef.

Jakovljević,

Kruszka,

Waligórski

et al. 2024

Physiologia Plantarum

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AimsDue to the lack of experimental databases, together with the chemical complexity and the dynamic nature of plants' metabolome, most of the metabolites in complex biological materials (like plant in vitro tissue cultures) are not‐annotated, unidentified metabolites. In this study, a method for further metabolite characterization and classification based on the UPLC‐HESI‐HRMS/MS approach for small‐leaved basil (Ocimum basilicum L. var. minimum Alef.) callus and cell suspension culture is presented.Key resultsA total of 2168 metabolic features were detected, out of which the database for exact mass metabolic profiling for 1949 metabolites is presented here since there is no available database dedicated to O. basilicum. We further focused on secondary metabolites (particularly phenolic compounds). The presence of 60 different phenolic compounds belonging mainly to the groups of flavonoids, glycosides, terpenoids, and phenolic acids is confirmed. By comparing relative abundances of phenolic compounds from callus culture and cell suspension culture, both grown on two types of media, via svd‐PCА, univariate analysis, post‐hoc tests, and heatmapping of metabolites, we provided a practical example of how resources presented here can be further applied in tissue culture‐based basil metabolomics studies.UtilityThis study represents the first approach toward routine targeted investigation of secondary metabolites in basil in vitro cultures and provides various opportunities for new‐generation analyses.

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Synergistic effects of salicylic acid and light stress on bioactive metabolites in basil callus cultures

Cited by 13 publications

References 67 publications

Biochemical responses and volatile compounds in a peppermint chemotype grown in a controlled environment

Biochemical responses and volatile compounds in a peppermint chemotype grown in a controlled environment

Enhancing Bioactive Metabolite Production in Aerva sanguinolenta Callus Cultures through Silver Nanoparticle and Salicylic Acid Elicitation

Untargeted metabolomic in basil cell cultures – a case study of Ocimum basilicum L. var. minimum Alef.

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