Synergistic Effects of l-Arginine and Methyl Salicylate on Alleviating Postharvest Disease Caused by Botrysis cinerea in Tomato Fruit

Abstract

The effects of l-arginine (Arg, 1 mM) and/or methyl salicylate (MeSA, 0.05 mM) treatment on gray mold caused by Botrytis cinerea in tomato fruit were studied. Results indicated that Arg or MeSA alleviated the incidence and severity of fruit disease caused by B. cinerea, and that both Arg and MeSA (Arg + MeSA) further inhibited the development of fruit decay. Treatment with Arg + MeSA not only enhanced the activities of superoxide dismutase, catalase, and peroxidase but also promoted the expression levels of pathogenesis-related protein 1 gene and the activities of defense-related enzymes of phenylalanine ammonia-lyase, polyphenol oxidase, β-1,3-glucanase, and chitinase during most of the storage periods, which were associated with lower disease incidence and disease index. In addition, the combined treatment elevated the levels of total phenolics, polyamines, especially putrescine, and nitric oxide. These observations suggest that treatment of fruit with Arg + MeSA is an effective and promising way to alleviate postharvest decays on a commercial scale.

Effect of methyl salicylate in combination with 1‐methylcyclopropene on postharvest quality and decay caused by Botrytis cinerea in tomato fruit

“…The expression of pathogenesis‐related protein 1 ( PR1 ) was measured by qRT‐PCR using SYBR Green I Master Mix (Toyobo, Osaka, Japan) on a LineGene 9600 detection system (Bioer, HangZhou, China). The specific primers of PR1 and the reference gene ( Ubi3 ) used for qRT‐PCR were as described previously . The qRT‐PCR program comprised a preliminary step of 2 min at 95 °C followed by 40 cycles of 15 s at 95 °C, 20 s at 60 °C and 45 s at 72 °C.…”

Nitric oxide in the physiology and quality of fleshy fruits

“…In Table 2, the impact of NO 1 treatment on plant disease resistance is briefly reviewed as it has been associated to the 1 induction of a series of defense-related genes/enzymes (Zheng et al, 2011a(Zheng et al, , 2017Yu et 1 al., 2012;Gu et al, 2014;Hu et al, 2014Hu et al, , 2019. Besides this effect on 1 the defense system, alterations of ROS and antioxidant metabolisms have been reported 1 as a consequence of the exogenous NO application in fruits (Fan et al, 2008;Lai et al, 1 2011; Zhang et al, 2011Zhang et al, , 2017bShi et al, 2015;Zhou et al, 2016) ( Table 2) have been found to induce disease resistance in diverse fruit species (Fan et al, 2008; 2 Shi et al, 2015;Zheng et al, 2014Zheng et al, , 2015.…”

SlARG2 contributes to MeJA‐induced defense responses to Botrytis cinerea in tomato fruit

“…GLU and CHI also are important pathogenesis‐related enzymes 38 . In addition, PR 1 , an indicator of systemic acquired resistance, also is an important PR family gene 22 . Our research indicated that MeJA treatment increased GLU activity by inducing the expression levels of PR2a and PR2b , and increased CHI activity by enhancing PR3b expression level, whereas PR3a expression was unchanged.…”