Synergistic effects of ultrashort wave and bone marrow stromal cells on nerve regeneration with acellular nerve allografts

ChaoJian, Pang; Tong, Lei; Ji, Lili; Wang, Zhenyu; Zhang, Xu; Gao, Hai; Hua, Jia; Zhang, Lixin; Tong, Xin‐Kang

doi:10.1002/syn.21669

Cited by 29 publications

(32 citation statements)

References 49 publications

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“…Cells suspended in culture medium can be microinjected into nerve ends or grafts (Figure 2A) [28,29,32,40,[158][159][160] . Although this delivers cells directly to the site of injury, the process of micro-injection can be traumatic to the delicate intra-neural architecture and can result in unpredictable cell distribution.…”

Section: Cell Scaffolds and Methods Of Deliverymentioning

confidence: 99%

Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

Fairbairn

Meppelink

Ng-Glazier

et al. 2015

WJSC

129

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Outcomes following peripheral nerve injury remain frustratingly poor. The reasons for this are multifactorial, although maintaining a growth permissive environment in the distal nerve stump following repair is arguably the most important. The optimal environment for axonal regeneration relies on the synthesis and release of many biochemical mediators that are temporally and spatially regulated with a high level of incompletely understood complexity. The Schwann cell (SC) has emerged as a key player in this process. Prolonged periods of distal nerve stump denervation, characteristic of large gaps and proximal injuries, have been associated with a reduction in SC number and ability to support regenerating axons. Cell based therapy offers a potential therapy for the improvement of outcomes following peripheral nerve reconstruction. Stem cells have the potential to increase the number of SCs and prolong their ability to support regeneration. They may also have the ability to rescue and replenish populations of chromatolytic and apoptotic neurons following axotomy. Finally, they can be used in non-physiologic ways to preserve injured tissues such as denervated muscle while neuronal ingrowth has not yet occurred. Aside from stem cell type, careful consideration must be given to differentiation status, how stem cells are supported following transplantation and how they will be delivered to the site of injury. It is the aim of this article to review current opinions on the strategies of stem cell based therapy for the augmentation of peripheral nerve regeneration.

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Section: Cell Scaffolds and Methods Of Deliverymentioning

confidence: 99%

Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

Fairbairn

Meppelink

Ng-Glazier

et al. 2015

WJSC

129

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“…The immunoreactive signals were visualized by goat anti-mouse IgG (Jackson, 1:200 dilution). In randomly selected high power fields, the integrated optical density (IOD) of a positive immunological reaction was analyzed by Image-Pro Plus 6.0 [32,33]. …”

Section: Methodsmentioning

confidence: 99%

Schwann-like cells seeded in acellular nerve grafts improve nerve regeneration

Fan

et al. 2014

BMC Musculoskelet Disord

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BackgroundThis study evaluated whether Schwann-like cells (SLCs) induced from bone marrow-derived mesenchymal stem cells (BM-MSCs) transplanted into acellular nerve grafts (ANGs) could repair nerve defects compared with nerve isografts and ANGs with BM-MSCs.MethodsBM-MSCs extracted, separated and purified from the bone marrow of rats, and some of the BM-MSCs were cultured with mixed induction agents that could induce BM-MSCs into SLCs. Either SLCs or BM-MSCs were seeded onto 10-mm ANGs, and the isografts were chosen as the control. The walking-track test, tibialis anterior muscle weight measurement, electrophysiological examination, toluidine blue staining, transmission electron micrographs and immunostaining of S-100 and VEGF in these three groups were evaluated in a 10-mm rat sciatic injury-repair model.ResultsThe walking-track test, tibialis anterior muscle weight measurement and electrophysiological examination of the sciatic nerve suggested the groups of ANGs with SLCs and isografts obtained better results than the BM-MSC group (P < 0.05). Meanwhile, the results of the SLCs and isograft groups were similar (P > 0.05). All the histomorphometric analyses (toluidine blue staining, transmission electron micrographs and immunostaining of S-100 and VEGF) showed that there were more regenerating nerve fibers in the group of ANGs with SLCs than the BM-MSCs (P < 0.05), but there was no significant difference between the SLC and isograft groups (P > 0.05).ConclusionsSLCs seeded in ANGs and isografts show better functional regeneration compared with BM-MSCs seeded in ANGs. Additionally, SLCs combined with ANGs present almost the same outcome as the isografts. Therefore, SLCs with ANGs can be a good choice in nerve defect repairs.

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“…Authors have tried various combinations of different nerve conduits and supporting cells to repair peripheral nerve injury. Pang et al 17. reported that acellular nerve allografts seeded with BM-MNC can be more effective in repairing peripheral nerves than acellular nerve allografts alone.…”

Section: Discussionmentioning

confidence: 99%

Chitosan nerve conduits seeded with autologous bone marrow mononuclear cells for 30 mm goat peroneal nerve defect

Muheremu

Chen

Wang

et al. 2017

Sci Rep

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In the current research, to find if the combination of chitosan nerve conduits seeded with autologous bone marrow mononuclear cells (BM-MNCs) can be used to bridge 30 mm long peroneal nerve defects in goats, 15 animals were separated into BM-MNC group (n = 5), vehicle group (n = 5), and autologous nerve graft group (n = 5). 12 months after the surgery, animals were evaluated by behavioral observation, magnetic resonance imaging tests, histomorphological and electrophysiological analysis. Results revealed that animals in BM-MNC group and autologous nerve graft group achieved fine functional recovery; magnetic resonance imaging tests and histomorphometry analysis showed that the nerve defect was bridged by myelinated nerve axons in those animals. No significant difference was found between the two groups concerning myelinated axon density, axon diameter, myelin sheath thickness and peroneal nerve action potential. Animals in vehicle group failed to achieve significant functional recovery. The results indicated that chitosan nerve conduits seeded with autologous bone marrow mononuclear cells have strong potential in bridging long peripheral nerve defects and could be applied in future clinical trials.

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Synergistic effects of ultrashort wave and bone marrow stromal cells on nerve regeneration with acellular nerve allografts

Cited by 29 publications

References 49 publications

Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

Schwann-like cells seeded in acellular nerve grafts improve nerve regeneration

Chitosan nerve conduits seeded with autologous bone marrow mononuclear cells for 30 mm goat peroneal nerve defect

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