Synergistic effects of UV photofunctionalization and micro-nano hybrid topography on the biological properties of titanium

Tsukimura, Naoki; Yamada, Masahiro; Iwasa, Fuminori; Minamikawa, Hajime; Att, Wael; Ueno, Takeshi; Saruwatari, Lei; Aita, Hideki; Chiou, W. A.; Ogawa, Takahiro

doi:10.1016/j.biomaterials.2011.03.001

Cited by 87 publications

(85 citation statements)

References 31 publications

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“…Titanium surfaces with enhanced microscale and nanoscale morphological features exhibited improved osteoblastic behavior, such as increased cell attachment, proliferation, alkaline phosphatase activity, and upregulated gene expression of bone-related proteins, when compared to surfaces with microfeatures alone. [4][5][6][7] Studies also revealed the new potential of nanofeatured titanium surfaces as a smart material for inducing cell-specific affinity, eg, selective attractiveness for osteogenic cells but not for fibroblasts.…”

Section: Introductionmentioning

confidence: 99%

Bone integration capability of nanopolymorphic crystalline hydroxyapatite coated on titanium implants

Yamada

Ueno

Tsukimura

et al. 2012

IJN

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Abstract:The mechanism by which hydroxyapatite (HA)-coated titanium promotes bone-implant integration is largely unknown. Furthermore, refining the fabrication of nanostructured HA to the level applicable to the mass production process for titanium implants is challenging. This study reports successful creation of nanopolymorphic crystalline HA on microroughened titanium surfaces using a combination of flame spray and low-temperature calcination and tests its biological capability to enhance bone-implant integration. Sandblasted microroughened titanium implants and sandblasted + HA-coated titanium implants were subjected to biomechanical and histomorphometric analyses in a rat model. The HA was 55% crystallized and consisted of nanoscale needle-like architectures developed in various diameters, lengths, and orientations, which resulted in a 70% increase in surface area compared to noncoated microroughened surfaces. The HA was free from impurity contaminants, with a calcium/phosphorus ratio of 1.66 being equivalent to that of stoichiometric HA. As compared to microroughened implants, HA-coated implants increased the strength of bone-implant integration consistently at both early and late stages of healing. HA-coated implants showed an increased percentage of bone-implant contact and bone volume within 50 µm proximity of the implant surface, as well as a remarkably reduced percentage of soft tissue intervention between bone and the implant surface. In contrast, bone volume outside the 50 µm border was lower around HA-coated implants. Thus, this study demonstrated that the addition of pure nanopolymorphic crystalline HA to microroughened titanium not only accelerates but also enhances the level of bone-implant integration and identified the specific tissue morphogenesis parameters modulated by HA coating. In particular, the nanocrystalline HA was proven to be drastic in increasing osteoconductivity and inhibiting soft tissue infiltration, but the effect was limited to the immediate microenvironment surrounding the implant.

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Section: Introductionmentioning

confidence: 99%

Bone integration capability of nanopolymorphic crystalline hydroxyapatite coated on titanium implants

Yamada

Ueno

Tsukimura

et al. 2012

IJN

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“…[13][14][15][16] A series of previous studies demonstrated that maintaining hydrophilic properties of titanium surfaces appears to have a positive effect on increasing attachment, spread, and proliferation of osteogenic cells; however, no evidence has been found that indicates the fact that hydrophilicity of a material is critical in determining its bioactivity or osteoconductivity. 1,2,5,10 Specifically, the bioactivity of titanium surfaces did not correlate with the level of their hydrophilicity as it changes during biological aging and UV photofunctionalization.…”

Section: Dovepressmentioning

confidence: 99%

“…Therefore, bone morphogenesis induced around UV-treated titanium surfaces could be defined as super osseointegration, 10,26 not just because of the UVinduced, highly osteoconductive surfaces, but due to a near complete block of soft tissue formation around these surfaces. The present study found that microtopographic surfaces are not able to maintain UV-induced high levels of bioactivity, but micro-nano-hybrid surfaces are able to maintain UV-induced even higher levels of bioactivity, at least for the 7 days they were monitored.…”

Section: Dovepressmentioning

confidence: 99%

“…A recent study demonstrated that UV treatment increases the biological property of micro-nano-hybrid surfaces. 10 The next crucial question would be whether and how UV-photofunctionalized micro-nano-hybrid surfaces are subjected to the biological aging. For instance, one simple but difficult question is whether the larger surface area of the micro-nano-hybrid surface compared to microfeatured surfaces has positive or negative effects on the progression of biological aging.…”

Section: Introductionmentioning

confidence: 99%

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TiO2 micro-nano-hybrid surface to alleviate biological aging of UV-photofunctionalized titanium

Iwasa

Tsukimura²,

Sugita³

et al. 2011

IJN

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Bioactivity and osteoconductivity of titanium degrade over time after surface processing. This time-dependent degradation is substantial and defined as the biological aging of titanium. UV treatment has shown to reactivate the aged surfaces, a process known as photofunctionalization. This study determined whether there is a difference in the behavior of biological aging for titanium with micro-nano-hybrid topography and titanium with microtopography alone, following functionalization. Titanium disks were acid etched to create micropits on the surface. Micro-nano-hybrid surfaces were created by depositioning 300-nm diameter TiO 2 nodules onto the micropits using a previously established self-assembly protocol. These disks were stored for 8 weeks in the dark to allow sufficient aging, then treated with UV light for 48 hours. Rat bone marrow–derived osteoblasts were cultured on fresh disks (immediately after UV treatment), 3-day-old disks (disks stored for 3 days after UV treatment), and 7-day- old disks. The rates of cell attachment, spread, proliferation, and levels of alkaline phosphatase activity, and calcium deposition were reduced by 30%–50% on micropit surfaces, depending on the age of the titanium. In contrast, 7-day-old hybrid surfaces maintained equivalent levels of bioactivity compared with the fresh surfaces. Both micropit and micro-nano-hybrid surfaces were superhydrophilic immediately after UV treatment. However, after 7 days, the micro-nano- hybrid surfaces became hydrorepellent, while the micropit surfaces remained hydrophilic. The sustained bioactivity levels of the micro-nano-hybrid surfaces were nullified by treating these surfaces with Cl − anions. A thin TiO 2 coating on the micropit surface without the formation of nanonodules did not result in the prevention or alleviation of the time-dependent decrease in biological activity. In conclusion, the micro-nano-hybrid titanium surfaces may slow the rate of time-dependent degradation of titanium bioactivity after UV photofunctionalization compared with titanium surfaces with microtopography alone. This antibiological aging effect was largely regulated by its sustained electropositivity uniquely conferred in TiO 2 nanonodules, and was independent of the degree of hydrophilicity. These results demonstrate the potential usefulness of these hybrid surfaces to effectively utilize the benefits of UV photofunctionalization and provide a model to explore the mechanisms underlying antibiological aging properties.

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“…1 The use of narrow implants and early or even immediate loading requires a higher degree of osseointegration at the early stage of healing. 2 A variety of surface modification methods have been developed to facilitate osseointegration of titanium implants, including control of the surface topography, 3 hydroxyapatite coating, 4 and bioactive molecule (BM) immobilization. 5,6 Over the last decade, various BM-based strategies have attracted a lot of attention.…”

Section: Introductionmentioning

confidence: 99%

Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation

Chen¹,

Han²,

Kong³

et al. 2014

IJN

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In this study, to improve the osseointegration of implants, osteogenic growth peptide (OGP) and fibronectin (FN) were loaded within mineral, which was formed on titanium, through adsorption and coprecipitation methods. The release profiles of OGP loaded by either adsorption or coprecipitation and the effects of the loading methods to immobilize OGP with and without FN on rat mesenchymal stem cell (rMSC) osteogenic differentiation were studied. The coprecipitation approach slightly reduced the initial burst release, while the adsorption approach provided a more sustained release. Dual loading of OGP and FN further improved cell attachments compared with either OGP or FN alone. Dually loaded OGP and FN also had a positive impact on rMSC proliferation and osteogenic differentiation. The difference in methods of loading OGP with and without FN also had some effects on osteogenic differentiation. Compared with coprecipitated OGP alone, adsorbed OGP enhanced later differentiation, such as osteocalcin secretion and matrix mineralization. Simultaneously adsorbed OGP and FN led to higher proliferation and higher osteogenic differentiation in both early and late stages compared with sequentially loaded OGP and FN. rMSC culture clearly indicated that simultaneously adsorbed OGP and FN could improve osseointegration, and this treatment represents a potential method for effective surface modification of dental and orthopedic implants.

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Synergistic effects of UV photofunctionalization and micro-nano hybrid topography on the biological properties of titanium

Cited by 87 publications

References 31 publications

Bone integration capability of nanopolymorphic crystalline hydroxyapatite coated on titanium implants

Bone integration capability of nanopolymorphic crystalline hydroxyapatite coated on titanium implants

TiO2 micro-nano-hybrid surface to alleviate biological aging of UV-photofunctionalized titanium

Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation

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