Synergistic Enhancement of Antitumor Efficacy by PEGylated Multi-walled Carbon Nanotubes Modified with Cell-Penetrating Peptide TAT

Hu, Shaozheng; Wang, Tong; Pei, Xibo; Cai, He; Chen, Junyu; Zhang, Xin; Wan, Qianbing; Wang, Jian

doi:10.1186/s11671-016-1672-6

Cited by 16 publications

(8 citation statements)

References 41 publications

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“…The Tat Oyi vaccine in association with combination anti-retroviral therapy may provide an efficient means of controlling the HIV-infected cell reservoir [ 71 ]. Furthermore, as a cell penetrating peptide, Tat has been developed as a drug delivery system in the treatment of cancer [ 72 ]. However, Tat and anti-cancer drugs both have cardiovascular toxicity.…”

Section: Discussionmentioning

confidence: 99%

The role of HIV Tat protein in HIV-related cardiovascular diseases

et al. 2018

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The human immunodeficiency virus (HIV) is a major global public health issue. HIV-related cardiovascular disease remains a leading cause of morbidity and mortality in HIV positive patients. HIV Tat is a regulatory protein encoded by tat gene of HIV-1, which not only promotes the transcription of HIV, but it is also involved in the pathogenesis of HIV-related complications. This review is aimed at summarizing the current understanding of Tat in HIV-related cardiovascular diseases.

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Section: Discussionmentioning

confidence: 99%

The role of HIV Tat protein in HIV-related cardiovascular diseases

et al. 2018

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“…Alternative PFC nanoemulsions were also formed with EYP surfactant, where cys-TAT was conjugated to commercially available 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-peg2000-maleimide 35 (Figure 1B), and the product 4 was confirmed by matrix assisted laser desorption/ionization mass spectrometry. Conjugate 4 was inserted into nanoemulsion either into the crude mix before emulsification ("direct insertion") or after emulsification ("post-insertion").…”

Section: Tat-pfc Nanoemulsionmentioning

confidence: 99%

Cell penetrating peptide functionalized perfluorocarbon nanoemulsions for targeted cell labeling and enhanced fluorine‐19 MRI detection

Hingorani

Chapelin

Stares

et al. 2019

Magnetic Resonance in Med

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Purpose: A bottleneck in developing cell therapies for cancer is assaying cell biodistribution, persistence, and survival in vivo. Ex vivo cell labeling using perfluorocarbon (PFC) nanoemulsions, paired with 19 F MRI detection, is a noninvasive approach for cell product detection in vivo. Lymphocytes are small and weakly phagocytic limiting PFC labeling levels and MRI sensitivity. To boost labeling, we designed PFC nanoemulsion imaging probes displaying a cell-penetrating peptide, namely the transactivating transcription sequence (TAT) of the human immunodeficiency virus. We report optimized synthesis schemes for preparing TAT co-surfactant to complement the common surfactants used in PFC nanoemulsion preparations. Methods: We performed ex vivo labeling of primary human chimeric antigen receptor (CAR) T cells with nanoemulsion. Intracellular labeling was validated using electron microscopy and confocal imaging. To detect signal enhancement in vivo, labeled CAR T cells were intra-tumorally injected into mice bearing flank glioma tumors. Results: By incorporating TAT into the nanoemulsion, a labeling efficiency of ~10 12 fluorine atoms per CAR T cell was achieved that is a >8-fold increase compared to nanoemulsion without TAT while retaining high cell viability (~84%). Flow cytometry phenotypic assays show that CAR T cells are unaltered after labeling with TAT nanoemulsion, and in vitro tumor cell killing assays display intact cytotoxic function.The 19 F MRI signal detected from TAT-labeled CAR T cells was 8 times higher than cells labeled with PFC without TAT. Conclusion: The peptide-PFC nanoemulsion synthesis scheme presented can significantly enhance cell labeling and imaging sensitivity and is generalizable for other targeted imaging probes.

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“…CNTs can be functionalized either by covalent or non-covalent modifications. [7][8][9][10] Covalent modifications is preferred because of its advantages with respect to solubility, safety and compatability. Currently the following three methods are preferred to functionalize MWCNTs.…”

Section: Introductionmentioning

confidence: 99%

HCT116 Cells Cytotoxic Response to Multifuctionalized 5- Fluorouracil MWCNTs Conjugates In Colorectal Cancer

K.P.¹

2021

ijcsrr

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Nanomaterials are the foundations of Nanotechnology, which are measured in nanoscales, Carbon nanotubes are one of the interesting nanomaterials, studied for over 25 years because of their superlative properties such as high surface area, electrical and thermal conductivity, high biocompatibility, flexibility, resistance to corrosion and nanosize. According to research, carbon nanotubes are applied in sensing, water treatment, and drug delivery, mainly used to deliver the anticancer drugs. In our work, functionalization of multi walled carbon nanotubes done by covalent and non-covalent functionation methods, covalent functionalization showed better dispersing efficiency in aqueous medium and compatible with biological systems with damaging the crystal lattice of carbon nanotubes. Non covalent functionalization helps to derivatized with active compounds, surface adsorption or attachment of various molecules or antibodies, which subsequently helps in targeting the site and to produce therapeutic effects. Different formulations prepared by functionalized MWCNTs and multiple functionalization of MWCNTs done by binding the drug and antibodies to prepare functionalized MWCNTs 5-Fluorouracil complexes. The Cytotoxicity assay was carried out for the obtained new targeting formulations to analyze the effect of all the formulations on HCT116 cell line. The percentage death was determined based on the viability of the cells in the appropriate vehicle controls. In this study, we report the successful functionalization, binding of 5 Fluorouracil, antibodies to MWCNTs, and cells viability of all prepared formulations for the development of novel carbon based anticancer drug delivery system. Functionalized MWCNTs-5-Fluorouracil antibodies composite at concentration above 2.5 µg/mL exhibited ≥ 50% cytotoxicity post normalization with compound control to negate precipitation observed with the compound. All the formulations showed the precipitations indicating antitumor activity and biocompatibility.

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Synergistic Enhancement of Antitumor Efficacy by PEGylated Multi-walled Carbon Nanotubes Modified with Cell-Penetrating Peptide TAT

Cited by 16 publications

References 41 publications

The role of HIV Tat protein in HIV-related cardiovascular diseases

The role of HIV Tat protein in HIV-related cardiovascular diseases

Cell penetrating peptide functionalized perfluorocarbon nanoemulsions for targeted cell labeling and enhanced fluorine‐19 MRI detection

HCT116 Cells Cytotoxic Response to Multifuctionalized 5- Fluorouracil MWCNTs Conjugates In Colorectal Cancer

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