Synergistic enhancement of human bone marrow stromal cell proliferation and osteogenic differentiation on BMP-2-derived and RGD peptide concentration gradients

Moore, Nicole M.; Lin, Nancy J.; Gallant, Nathan D.; Becker, Matthew L.

doi:10.1016/j.actbio.2011.01.019

Cited by 116 publications

(133 citation statements)

References 58 publications

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“…Increased expression of Runx2 by cells bound to Coll I and BMP 2 has been observed in previous studies [10,18,48,49,65,66]. Coll I has been described to prompt calcification of the stromal cell matrix after three weeks of MSC culture, suggesting that contact with ECM proteins alone stimulates differentiation [8], This is in line with reports showing that MSCs undergo osteoblast differentiation when in contact with collagen-containing ECM proteins in both in vitro and in vivo settings [9,67].…”

Section: Discussionsupporting

confidence: 83%

Synergistic influence of collagen I and BMP 2 drives osteogenic differentiation of mesenchymal stem cells: A cell microarray analysis

et al. 2016

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Section: Discussionsupporting

confidence: 83%

Synergistic influence of collagen I and BMP 2 drives osteogenic differentiation of mesenchymal stem cells: A cell microarray analysis

et al. 2016

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“…Among the peptides that mediate cell adhesion, arginine-glycine-aspartic acid (RGD), as found in fibronectin and laminin, is the most commonly applied cell-binding peptide for substrate modification in order to enhance cell adhesion [20][21][22][23][24][25][26][27]. Cell behavior on RGD-conjugated substrates depend on peptide densities [28][29]. It is reported that RGD nano-spacing is crucial to specific adhesion of cells [30][31] and the differentiation of stem cells [32].…”

mentioning

confidence: 99%

Modulation of the stemness and osteogenic differentiation of human mesenchymal stem cells by controlling RGD concentrations of poly(carboxybetaine) hydrogel

Chien

Shih

et al. 2014

Biotechnology Journal

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In vitro modulation of the differentiation status of mesenchymal stem cells (MSCs) is important for their application to regenerative medicine. We suggested that the morphology and differentiation states of MSCs could be modulated by controlling the cell affinity of a substrate. The objective of this study was to investigate the effects of surface bio-adhesive signals on self-renewal and osteogenic differentiation of MSCs using a low-fouling platform. Cell-resistant poly(carboxybetaine) hydrogel was conjugated with 5 μM or 5 mM of cell-adhesive arginine-glycine-aspartic acid (RGD) peptides in order to control the cells' affinity to the substrate. Human mesenchymal stem cells (hMSCs) were cultured on the RGD-modified poly(carboxybetaine) hydrogel and then the cells' states of stemness and osteogenic differentiation were evaluated using reverse-transcriptase polymerase chain reaction. The hMSCs formed three-dimensional spheroids on the 5 μM RGD substrate, while cells on the 5 mM RGD substrate exhibited spreading morphology. Furthermore, cells on the 5 μM RGD hydrogel maintained a better stemness phenotype, while the hMSCs on the 5 mM RGD hydrogel proliferated faster and underwent osteogenic differentiation. In conclusion, the stemness of hMSCs was best maintained on a low RGD surface, while osteogenic differentiation of hMSCs was enhanced on a high RGD surface. monolayer culture [13][14], while MSCs with spheroid morphology tend to sustain pluripotent ability during extended culturing [14][15][16]. Cell adhesion to the extracellular matrix (ECM) in a native tissue is initiated by the binding of cell membrane receptors (e.g. integrins) to ECM adhesion proteins such as fibronectin and laminin [17]. Similarly, cell attachment to artificial substrates is usually mediated by surfacebound adhesion proteins. On the other hand, cell-cell attachment is mediated by other types of cell receptors such as cadherins [18]. We suggest that cell morphology during in vitro culture could be determined by the relative affinity between cell-substrate and cell-cell interactions. When the cell-substrate interaction is stronger than the cell-cell interaction, cells attach to and spread on the substrate. Conversely, when the cell-substrate interaction is weaker than the cell-cell interaction, cells tend to form spheroids on the surface [19].We suggested that by controlling the cell affinity of a substrate the morphology and differentiation status of MSCs could be modulated. To this end, surface conjugation of ECM adhesion proteins such as fibronectin, collagen, and laminin, is a common strategy. Alternatively, conjugation of peptides containing cell-binding domains of ECM adhesion proteins is another popular approach. The advantages of peptides compared to intact proteins include cost effectiveness and reduced vulnerability to denaturation. Among the peptides that mediate cell adhesion, arginine-glycine-aspartic acid (RGD), as found in fibronectin and laminin, is the most commonly applied cell-binding peptide for substrate modification in ...

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“…reports have demonstrated that BMP-2 can stimulate the proliferation, migration and osteogenic differentiation of mesenchymal stem cells [20][21][22][23] . Moreover, BMP-2 has been proven to induce osteogenesis in vivo [18,24] .…”

Section: Bmp-2 Is An Important Growth Factor At the Process Of Osteogmentioning

confidence: 98%

“…In the deposition at negative potential, these negatively charged collagen fibrils are repelled from the substrate to a location with a relative lower pH, i.e. the isoelectric point, and the collagen fibrils undergo 21 self-assembly, mineralization and deposition. If these negatively charged collagen fibrils under positive potential are directly deposited on substrate without self-assembly, low mineralized collagens should be increased in the resulting coatings.…”

Section: Mechanism Of Ap-ecdmentioning

confidence: 99%

Alternating potentials assisted electrochemical deposition of mineralized collagen coatings

Zhuang

Lin

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Synergistic enhancement of human bone marrow stromal cell proliferation and osteogenic differentiation on BMP-2-derived and RGD peptide concentration gradients

Cited by 116 publications

References 58 publications

Synergistic influence of collagen I and BMP 2 drives osteogenic differentiation of mesenchymal stem cells: A cell microarray analysis

Synergistic influence of collagen I and BMP 2 drives osteogenic differentiation of mesenchymal stem cells: A cell microarray analysis

Modulation of the stemness and osteogenic differentiation of human mesenchymal stem cells by controlling RGD concentrations of poly(carboxybetaine) hydrogel

Alternating potentials assisted electrochemical deposition of mineralized collagen coatings

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