Synergistic Evaluation of &amp;lt;i&amp;gt;Moringa oleifera, Hunteria umbellate&amp;lt;/i&amp;gt; and &amp;lt;i&amp;gt;Azadirachta indica&amp;lt;/i&amp;gt; with Antibiotics Against Environmental MRSA Isolates: An &amp;lt;i&amp;gt;In-vitro&amp;lt;/i&amp;gt; Study

Peter, Akinrotoye Kehinde; Paul, Akinduti; Olabisi, Lanlokun; Clement, Adetogun

doi:10.11648/j.ajbio.20200804.11

Cited by 4 publications

(2 citation statements)

References 24 publications

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“…Several studies have shown the presence of different secondary metabolite like: alkaloids, volatile oils, tannins, flavonoids and saponins etc in the different extracts of H. umbellata [27][28][29].…”

Section: Discussionmentioning

confidence: 99%

“…Anibijuwon et al[37] study shows that ethanol and methanol seed extracts of H. umbellata have a zone of inhibition of 30 and 17 mm respectively against Streptococcus sp. Akinrotoye et al[28] study shows that the agar diffusion assay of H. umbellata extract-Azithromycin combination had the least zones of inhibition ≥21.00±1.92 mm in 75% of all isolates tested.…”

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confidence: 96%

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Phytochemical Screening, Mathematical Analysis and Antimicrobial Activity of Methanolic Seed Extract of Hunteria Umbellata

Aderele

Rasaq

Momoh

2020

EJMP

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Aim: The study evaluates the in-vitro antimicrobial activity of Hunteria umbellata against Escherichia coli, Staphylococcus aureus and Streptococcus sp. Place and Duration of Study: The study was carried out for three months in 2019 in Biochemistry Laboratory, Department of Chemical Sciences (Biochemistry unit), School of Pure and Applied Sciences, Lagos State Polytechnic, Ikorodu, Lagos- Nigeria. Methodology: The qualitative and GC-MS analysis of Hunteria umbellata methanolic seed extract were determined using standard procedure. The antimicrobial activity was evaluated by the disc diffusion method and agar well diffusion method. The experimental data was resampled 1000 times to allow for higher degrees of freedom in carrying out t-test to test for the difference of the effect of in-vitro antimicrobial activity of H. umbellata against E. coli, S. aureus and Streptococcus sp using mathematical software R language (3.6.1 version). Line plots, histogram and t-test are used to explain the effect of antimicrobial activity of H. umbellate on the selected bacteria. MIC and MBC were determined using standard methods. Results: The Phytochemical analysis of methanolic seed extract of Hunteria umbellata showed the presence of secondary metabolites like saponins, tannins, flavonoids, steroids, phenol among others. GC-MS assay of the H. umbellata seed extract revealed the presence of eight different compounds. Agar well diffusion method was characterized by inhibition zones of 18.36±0.87, 19.13±1.03 and 21.62±2.53 mm for E.coli, S. aureus and Streptococcus sp respectively at 300 mg/ml-1 and 21.70± 1.60, 23.83± 2.64 and 28.57± 1.52 for E.coli, S. aureus and Streptococcus sp respectively at 500 mg/ml. The results of the analysis show that there is a significant difference between the effects of in-vitro antimicrobial activity of H. umbellate on 3001 and 500 mg/ml on each bacteria tested at 5% level of significance. E.coli, S. aureus and Streptococcus sp were tested against 12 standard antimicrobial agents, of which six was sensitive and another six was resistance to E .coli, seven was sensitive, and five was resistance to S. aureus while four was resistance and eight sensitive to Streptococcus sp. The minimum inhibitory concentration (MIC) for E.coli, S. aureus, and Streptococcus sp were 250, 125 and 31.25 mgml-1 while their minimum bactericidal concentration (MBC) were 500, 250 and 125 respectively. MIC and MBC tests showed that H. umbellata methanolic seed extract had noticeable bactericidal effects with MBC/MIC values ranging between 2 to 4. The extract has strong potency against these microorganisms with Streptococcus sp being the most susceptible. Conclusions: Hunteria umbellata has potential as natural therapeutic agents against E. coli, S. aureus and Streptococcus sp and they may prevent pathogenic diseases.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 96%

Phytochemical Screening, Mathematical Analysis and Antimicrobial Activity of Methanolic Seed Extract of Hunteria Umbellata

Aderele

Rasaq

Momoh

2020

EJMP

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In vitro ethno-toxicology of Hunteria umbellata methanol seed and stem extracts against Vibrio parahaemolyticus and its molecular implication on swarming and adhesion genes

Salisu,

Yahaya,

Fowora

et al. 2023

Bull Natl Res Cent

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Background Hunteria umbellatta (HU) is a predominant plant with a share of therapeutic properties against various human diseases with no scientific report of its in vitro ethno-toxicology coupled with molecular implication in the literature. This study, therefore, evaluated in vitro toxicological activity and quantification of expression of specific swarming gene, lateral flagellar (Laf A) and adhesion gene (ExsE) of Vibrio parahaemolyticus (VP) treated with methanol seed and stem extracts of HU at 500, 250, 125, 62.5, 31.25, 15.625, 7.8125, 3.90625 µg/µL concentrations. Results The toxicological results of all the eight tested concentrations from as low as 3.90625 µg/µL to as high as 500 µg/µL of HU seed and stem extracts revealed significantly (p ˂ 0.05) uninhibited bacterial growth in a dose-dependent manner compared to controls (positive and negative). The quantity of Laf A and ExsE genes’ expressions in VP was significantly higher (p ˂ 0.05) at 500 µg/µL of HU seed and stem compared to control while at 125 µg/µL of the same extract (seed and stem) showed significantly lowered (p ˂ 0.05) expression of swarming and adhesion genes in VP relative to control. Comparative to control, adhesion gene (ExsE) expression in VP significantly increased (p ˂ 0.05) at 250 µg/µL of HU seed and stem extracts. Conclusions The results obtained suggest toxicity at varying concentrations and higher concentration dosing of HU seed and stem is harmful as it could lead to increased expression of the gene colonization factor of VP as a major contributory agent of gastroenteritis.

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Phytochemical screening, gas chromatograph/mass spectrometer (GCMS) analysis and molecular toxicological potential of Hunteria umbellata aqueous fruit extract against Staphylococcus aureus in accessory gene regulators (AGRs)

Salisu,

Fowora,

Yahaya

et al. 2024

Futur J Pharm Sci

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Background Fruits of Hunteria umbellata (HU) have been utilized in folk medicine as potent against Staphylococcus aureus (SA) infections, particularly skin and nasal related conditions. However, there is scarcity of literature concerning toxicological evaluation of graded doses of HU fruit, especially at molecular level, specifically targeting the accessory gene regulator (AGR) system to prevent abuse of doses in the treatment of bacterial infections. This research evaluated molecular toxicological property of SA exposed to varied concentrations of aqueous HU fruit extract ranging from 500 to 3.90625 µg/µL using broth microdilution method and quantification of AGR I and II genes’ expression employing two-step reverse transcriptase quantitative polymerase chain reaction (RT-qPCR). We first identified quality and quantity of chemical compounds in aqueous fruit of HU using phytochemistry and hybrid gas chromatograph–mass spectrometer (GC–MS) technique. Additionally, preliminary bactericidal potential of HU was assessed before molecular toxicology. Results Results revealed six phytochemicals and twenty analytical grade compounds from a standard library were identified from chromatograms of HU fruit extract. Some main compounds detected are n-Hexadecanoic acid (25.24%), 2-Pentadecanone, 6,10,14-trimethyl (16.08%), Cuparene (16.63%), Tetradecanoic acid (6.21%) and 9-Octadecenoic acid, (E)- (5.70%). Bactericidal activity shows significant (p < 0.05) toxicity in the tested (8) concentrations of HU fruits in a dose-response relationship compared to the controls (positive and negative). The quantified expression of AGR I and II genes in SA was most significantly increased (p < 0.05) at both 250 and 500 µg/µL of HU fruit extract while least significant increase (p < 0.05) was recorded at 125 µg/µL compared to control. Conclusions Notably, the study highlighted a potential risk of augmented bacterial infection especially with higher doses of HU extracts during boils’ treatment and other epidermal infections instigated by Staph. Expression of both AGR genes at higher doses (250 and 500 µg/µL) is indicative of further expression of several other genes responsible for virulence factors in a variety of human bacterial infections. Thus, consumption of HU fruit to treat boils and blisters should be with great caution especially at high doses.

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Synergistic Evaluation of <i>Moringa oleifera, Hunteria umbellate</i> and <i>Azadirachta indica</i> with Antibiotics Against Environmental MRSA Isolates: An <i>In-vitro</i> Study

Cited by 4 publications

References 24 publications

Phytochemical Screening, Mathematical Analysis and Antimicrobial Activity of Methanolic Seed Extract of Hunteria Umbellata

Phytochemical Screening, Mathematical Analysis and Antimicrobial Activity of Methanolic Seed Extract of Hunteria Umbellata

In vitro ethno-toxicology of Hunteria umbellata methanol seed and stem extracts against Vibrio parahaemolyticus and its molecular implication on swarming and adhesion genes

Phytochemical screening, gas chromatograph/mass spectrometer (GCMS) analysis and molecular toxicological potential of Hunteria umbellata aqueous fruit extract against Staphylococcus aureus in accessory gene regulators (AGRs)

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