Synergistic growth stimulation of corneal fibroblasts by components of mesodermal growth factor from murine submaxillary glands

Haraguchi, Kenneth H.; Knox, Robert; Weimar, Virginia; Anderson, Ronald

doi:10.1002/jcp.1041110202

Cited by 7 publications

(6 citation statements)

References 34 publications

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“…This is consistent with the earlier finding that the esteropeptidase activity of MGF-I could be totally inhibited by preincubation with the kallikrein inhibitor aprotinin(20). In addition to the kallikrein component, there are two bioactive components in MGF-I1 that elute from a reverse-phase chromatography column at lower acetonitrile concentrations than classic EGF.…”

supporting

confidence: 90%

Stimulation of corneal endothelial wound healing in cats by mesodermal growth factor

Louiselle

et al. 1990

Current Eye Research

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Several growth factors have been evaluated for their effects on corneal wound healing but few studies have yet demonstrated an acceleration of endothelial repair in vivo. Mesodermal Growth Factor (MGF) was tested in vivo by making standardized freeze wounds in cat corneas and immediately injecting one of four concentrations of MGF in sterile phosphate buffered saline (PBS), or PBS alone, into the anterior chamber. Seven days later, the animals were sacrificed and the corneas excised and stained. Descemet's membrane and endothelium were dissected and mounted onto glass slides. The wound areas were photographed, measured and compared statistically. Those cats receiving the three lowest doses of MGF had significantly smaller wounds than controls (p less than 0.05).

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supporting

confidence: 90%

Stimulation of corneal endothelial wound healing in cats by mesodermal growth factor

Louiselle

et al. 1990

Current Eye Research

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“…More recent studies, dealing with the purification of mesodermal growth factor(s) originating from mouse submaxillary gland and active on the proliferation of rabbit corneal fibroblasts, did not show any clear relationship between mitogenic and arginylesteropeptidase activities (Haraguchi et al, 1982). On the other hand, the growth-enhancing effect of factors partially characterized from rat submaxillary gland and active on c 6 glioma cells and 3T3 cells appeared to be unrelated to esteropeptidase activity present in these extracts (McClure et al, 1981).…”

Section: Discussionmentioning

confidence: 95%

Growth‐promoting activity in serum‐free medium of kallikreinlike arginylesteropeptidases from rat submaxillary gland

Catalioto

Négrel

Gaillard

et al. 1987

Journal Cellular Physiology

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The characterization and purification of the growth-promoting activity present in rat submaxillary gland extracts, known to be required for the proliferation of adipose precursor cells in serum-free medium, have been undertaken. Fractionation of the extracts by ion-exchange chromatography, gel filtration, and affinity chromatography on immobilized benzamidine allowed the copurification of the mitogenic activity with two distinct arginylesteropeptidases of apparent molecular weight 25,000; one of these enzymes has been purified to homogeneity and shown to be immunologically related to tonin, a well-characterized kallikreinlike protease from submaxillary gland. The specificity of both enzymes was similar to that of plasma and glandular kallikreins, as indicated by the relative rates of hydrolysis of peptide p-nitroanilide substrates. Prior treatment of the kallikreinlike proteases with phenylmethylsulfonylfluoride or aprotinin abolished completely both mitogenic and arginylesteropeptidase activities, indicating that enzymatic activity was essential for the manifestation of their growth-promoting ability. The kallikreinlike proteases from rat submaxillary gland were able to replace thrombin to support the proliferation of Chinese hamster lung fibroblasts in serum-free medium. These results underline the role of proteases in controlling cell growth and are discussed in light of adipose tissue development.

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“…Chromatographic fractionation of MGF yields four components, none of which has a biologic potency greater than that of the parental MGF. However, recombination of two components, MGF-I and -11, synergistically stimulates the response of keratocytes cultured from rabbit or rat corneas to a greater extent than does the parent MGF (Haraguchi et al, 1982).…”

Section: Mesodermal Growth Factormentioning

confidence: 94%

“…Mesodermal growth factor (MGF) is a pool of proteins, with a molecular weight of 26 kDa, that have been isolated by recycling gel chromatography of mouse submaxillary gland extracts and that do not contain NGF-like or EGF-like activity (Haraguchi et al, 1982). Chromatographic fractionation of MGF yields four components, none of which has a biologic potency greater than that of the parental MGF.…”

Section: Mesodermal Growth Factormentioning

confidence: 99%

The role of growth factors in the embryogenesis and differentiation of the eye

Tripathi

Livingston

et al. 1991

Am. J. Anat.

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The vertebrate eye is composed of a variety of tissues that, embryonically, have their derivation from surface ectoderm, neural ectoderm, neural crest, and mesodermal mesenchyme. During development, these different types of cells are subjected to complex processes of induction and suppressive interactions that bring about their final differentiation and arrangement in the fully formed eye. With the changing concept of ocular development, we present a new perspective on the control of morphogenesis at the cellular and molecular levels by growth factors that include fibroblast growth factors, epidermal growth factor, nerve growth factor, platelet-derived growth factor, transforming growth factors, mesodermal growth factors, transferrin, tumor necrosis factor, neuronotrophic factors, angiogenic factors, and antiangiogenic factors. Growth factors, especially transforming growth factor-beta, have a crucial role in directing the migration and developmental patterns of the cranial neural-crest cells that contribute extensively to the structures of the eye. Some growth factors also exert an effect on the developing ocular tissues by influencing the synthesis and degradation of the extracellular matrix. The mRNAs for the growth factors that are involved in the earliest aspects of the growth and differentiation of the fertilized egg are supplied from maternal sources until embryonic tissues are able to synthesize them. Subsequently, the developing eye tissues are exposed to both endogenous and exogenous growth factors that are derived from nonocular tissues as well as from embryonic fluids and the systemic circulation. The early interaction between the surface head ectoderm and the underlying chordamesoderm confers a lens-forming bias on the ectoderm; later, the optic vesicle elicits the final phase of determination and enhances differentiation by the lens. After the blood-ocular barrier is established, the internal milieu of the eye is controlled by the interactions among the intraocular tissues; only those growth factors that selectively cross the barrier or that are synthesized by the ocular tissues can influence further development and differentiation of the cells. An understanding of the tissue interactions that are regulated by growth factors could clarify the precise mechanism of normal and abnormal ocular development.

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Synergistic growth stimulation of corneal fibroblasts by components of mesodermal growth factor from murine submaxillary glands

Cited by 7 publications

References 34 publications

Stimulation of corneal endothelial wound healing in cats by mesodermal growth factor

Stimulation of corneal endothelial wound healing in cats by mesodermal growth factor

Growth‐promoting activity in serum‐free medium of kallikreinlike arginylesteropeptidases from rat submaxillary gland

The role of growth factors in the embryogenesis and differentiation of the eye

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