Synergistic Impacts of Arsenic and Antimony Co-contamination on Diazotrophic Communities

Li, Yongbin; Lin, Hanzhi; Gao, Pin; Nie, Yao; Xu, Rui; Sun, Xiaoxu; Li, Baoqin; Xu, Fuqing; Wang, Xiaoyu; Song, Benru; Sun, Weimin

doi:10.1007/s00248-021-01824-6

Cited by 13 publications

(11 citation statements)

References 115 publications

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“…Taxonomically related protists can serve different functions from microbial predators (consumers) to plant parasites and pathogens, and similarly, organisms that belong to different taxonomic groups may have developed to share similar patterns of nutritional feeding . Therefore, studying not only the taxonomy but also the potential function of protists would provide invaluable information. ,, In addition, the co-occurrence network, in ecosystem studies, has become an ever more important tool for exploring the symbiosis modes of the microbial community and identifying keystone taxa. ,, It has been reported that the biotic interactions of microorganisms are the most essential factors in the microbial community composition and function . The identification of keystone taxa is also critical because these taxa are essential for regulating the structure, composition, and functions of microbial communities and for facilitating the revegetation because they may be beneficial for plant growth and development …”

Section: Discussionmentioning

confidence: 99%

“…5,6 However, the tailings not only cover a large amount of mining land resource area and waste precious resources but also cause catastrophic environmental events due to the leaching of toxic metal(loid)s. 7 Therefore, it is crucial to improve the management of tailings using appropriate management and treatment strategies. 8 Bioremediation provides a promising remediation strategy at contaminated tailing sites because some microorganisms may contribute substantially to a wide range of ecological services, including nitrogen fixation, 9 carbon fixation, 10 and metal(loid) cycling, 11 transformation and pollution tolerance; these bacteria were even beneficial for phytoremediation by providing nutrients for plants. 10 Some bacteria that evolved to fix nitrogen autotrophically in response to nutrient deficiencies (e.g., nitrogen and carbon) were often detected in tailings, which could play important roles in facilitating nutrient acquisition by plants and ecological succession in tailings.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Bioremediation provides a promising remediation strategy at contaminated tailing sites because some microorganisms may contribute substantially to a wide range of ecological services, including nitrogen fixation, carbon fixation, and metal(loid) cycling, thereby affecting the ecosystem of tailing sites and promoting tailing vegetation. For instance, Meiothermus and Thiobacillus from alkaline tailings have shown potential as nitrogen- and carbon-fixing organisms as well as in metal transformation and pollution tolerance; these bacteria were even beneficial for phytoremediation by providing nutrients for plants .…”

Section: Introductionmentioning

confidence: 99%

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Primary Succession Changes the Composition and Functioning of the Protist Community on Mine Tailings, Especially Phototrophic Protists

Gao

Sun

et al. 2022

ACS Environ. Au

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Primary succession in mine tailings is a prerequisite for tailing vegetation. Microorganisms, including bacteria, fungi, and protists, play an important role in this process in the driving force for improving the nutritional status. Compared to bacteria and fungi, protist populations have rarely been investigated regarding their role in mine tailings, especially for those inhabiting tailings associated with primary succession. Protists are the primary consumers of fungi and bacteria, and their predatory actions promote the release of nutrients immobilized in the microbial biomass, as well as the uptake and turnover of nutrients, affecting the functions of the wider ecosystems. In this study, three different types of mine tailings associated with three successional stages (original tailings, biological crusts, and Miscanthus sinensis grasslands) were selected to characterize the protistan community diversity, structure, and function during primary succession. Some members classified as consumers dominated the network of microbial communities in the tailings, especially in the original bare land tailings. The keystone phototrophs of Chlorophyceae and Trebouxiophyceae showed the highest relative abundance in the biological crusts and grassland rhizosphere, respectively. In addition, the co-occurrences between protist and bacterial taxa demonstrated that the proportion of protistan phototrophs gradually increased during primary succession. Further, the metagenomic analysis of protistan metabolic potential showed that abundances of many functional genes associated with photosynthesis increased during the primary succession of tailings. Overall, these results suggest that the primary succession of mine tailings drives the changes observed in the protistan community, and in turn, the protistan phototrophs facilitate the primary succession of tailings. This research offers an initial insight into the changes in biodiversity, structure, and function of the protistan community during ecological succession on tailings.

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Section: Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Primary Succession Changes the Composition and Functioning of the Protist Community on Mine Tailings, Especially Phototrophic Protists

Gao

Sun

et al. 2022

ACS Environ. Au

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“…Mine tailings are extreme oligotrophic terrestrial environments, with nitrogen (N) as a limiting factor for the growth of plants and microorganisms, constraining the efficiency of bioremediation of mine tailing sites. − Biological N fixation (BNF) in mine tailings has important environmental implications, which may indirectly contribute to bioremediation or phytoremediation. − Diazotrophs play an important role in providing bioavailable N, particularly in oligotrophic environments. Compared to the extensive studies of N fixation in marine and soil environments, the understanding of the N fixation in mine tailings is still limited.…”

Section: Introductionmentioning

confidence: 99%

Serratia spp. Are Responsible for Nitrogen Fixation Fueled by As(III) Oxidation, a Novel Biogeochemical Process Identified in Mine Tailings

Guo

Häggblom

et al. 2022

Environ. Sci. Technol.

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Biological nitrogen fixation (BNF) has important environmental implications in tailings by providing bioavailable nitrogen to these habitats and sustaining ecosystem functions. Previously, chemolithotrophic diazotrophs that dominate in mine tailings were shown to use reduced sulfur (S) as the electron donor. Tailings often contain high concentrations of As(III) that might function as an alternative electron donor to fuel BNF. Here, we tested this hypothesis and report on BNF fueled by As(III) oxidation as a novel biogeochemical process in addition to BNF fueled by S. Arsenic (As)-dependent BNF was detected in cultures inoculated from As-rich tailing samples derived from the Xikuangshan mining area in China, as suggested by nitrogenase activity assays, quantitative polymerase chain reaction, and 15 N 2 enrichment incubations. As-dependent BNF was also active in eight other As-contaminated tailings and soils, suggesting that the potential for As-dependent BNF may be widespread in As-rich habitats. DNAstable isotope probing identified Serratia spp. as the bacteria responsible for Asdependent BNF. Metagenomic binning indicated that the essential genes for As-dependent BNF [i.e., nitrogen fixation, As(III) oxidation, and carbon fixation] were present in Serratia-associated metagenome-assembled genomes. Over 20 Serratia genomes obtained from NCBI also contained essential genes for both As(III) oxidation and BNF (i.e., aioA and nif H), suggesting that Asdependent BNF may be a widespread metabolic trait in Serratia spp.

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“…22,23 Our previous studies indicated diverse diazotrophic communities in this region. 24,25 Therefore, the investigation of Sb-dependent BNF has important environmental implications for improving N fertilizer efficiency and managing Sb toxicity in the environment.…”

Section: ■ Introductionmentioning

confidence: 99%

Chemolithotrophic Biological Nitrogen Fixation Fueled by Antimonite Oxidation May Be Widespread in Sb-Contaminated Habitats

Guo

Yang

et al. 2022

Environ. Sci. Technol.

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Nitrogen (N) deficiency in mining-contaminated habitats usually hinders plant growth and thus hampers tailing revegetation. Biological N fixation (BNF) is an essential biogeochemical process that contributes to the initial accumulation of N in oligotrophic mining-contaminated regions. Previous studies reported that chemolithotrophic rather than heterotrophic diazotrophs frequently dominated in the mining-contaminated regions. Chemolithotrophic diazotrophs may utilize elements abundant in such habitats (e.g., sulfur (S), arsenic (As), and antimony (Sb)) as electron donors to fix N 2 . BNF fueled by the oxidation of S and As has been detected in previous studies. However, BNF fueled by Sb(III) oxidation (Sb-dependent BNF) has never been reported. The current study observed the presence of Sb-dependent BNF in slurries inoculated from Sb-contaminated habitats across the South China Sb belt, suggesting that Sb-dependent BNF may be widespread in this region. DNA-stable isotope probing identified bacteria associated with Rhodocyclaceae and Rhizobiaceae as putative microorganisms responsible for Sb-dependent BNF. Furthermore, metagenomicbinning demonstrated that Rhodocyclaceae and Rhizobiaceae contained essential genes involved in Sb(III) oxidation, N 2 fixation, and carbon fixation, suggesting their genetic potential for Sb-dependent BNF. In addition, meta-analysis indicated that these bacteria are widespread among Sb-contaminated habitats with different niche preferences: Rhodocyclaceae was enriched in river sediments and tailings, while Rhizobiaceae was enriched only in soils. This study may broaden our fundamental understanding of N fixation in Sbmining regions.

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Synergistic Impacts of Arsenic and Antimony Co-contamination on Diazotrophic Communities

Cited by 13 publications

References 115 publications

Primary Succession Changes the Composition and Functioning of the Protist Community on Mine Tailings, Especially Phototrophic Protists

Primary Succession Changes the Composition and Functioning of the Protist Community on Mine Tailings, Especially Phototrophic Protists

Serratia spp. Are Responsible for Nitrogen Fixation Fueled by As(III) Oxidation, a Novel Biogeochemical Process Identified in Mine Tailings

Chemolithotrophic Biological Nitrogen Fixation Fueled by Antimonite Oxidation May Be Widespread in Sb-Contaminated Habitats

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