Synergistic influence of probiotic and florfenicol on embryonic viability, performance, and multidrug-resistant Salmonella Enteritidis in broiler chickens

Abstract

This study was conducted during the period of August 2021 to April 2022 and divided into two parts. The first part involved the isolation and characterization of Salmonella from 200 diseased broiler chickens collected from farms in Dakahlia Governorate, Egypt, with the detection of its antimicrobial susceptibility. The second experimental part involved in ovo inoculation of probiotics and florfenicol to evaluate their effects on hatchability, embryonic viability, growth performance traits and the control of multidrug-resistant Salmonella Enteritidis infections post hatching. The point prevalence of Salmonella in the internal organs of diseased chickens was 13% (26/200), including 6 serotypes: S. Enteritidis, S. Typhimurium, S. Santiago, S. Colindale, S. Takoradi and S. Daula. Multidrug resistance was found in 92% (24/26) of the isolated strains with a multiantibiotic resistance index of 0.33–0.88 and 24 antibiotic resistance patterns. The in ovo inoculation of probiotic with florfenicol showed significant improvement in the growth performance parameters compared with other groups and had the ability to prevent colonization of multidrug resistant S. Enteritidis in the majority of the experimental chicks, and the remaining chicks showed very low colonization, as detected by RT‒PCR. These findings suggested the application of in ovo inoculation techniques with both probiotics and florfenicol as a promising tool to control multidrug-resistant S. Enteritidis in poultry farms.

Биологическая И Экологическая Характеристика Возбудителя Сальмонеллеза Сельскохозяйственных Кур

“…Chicken infection with salmonellosis leads to huge economic losses, and the presence of Salmonella in chicken products hinders international trade. Therefore, in order to prevent Salmonella infection in poultry, additional costs for epidemiological surveillance, prevention and control are required [8][9][10].…”

“…Заражение сельскохозяйственных кур сальмонеллезом приводит к огромным экономическим потерям, а присутствие сальмонеллы в куроводческих продуктах препятствует международной торговле. Поэтому для предотвращения на птицефабриках заражения сельскохозяйственных кур сальмонеллой требуются дополнительные затраты на эпидемиологический надзор, профилактику и контроль [8][9][10].…”

Ecological prevalence, genetic diversity, and multidrug resistance of Salmonella enteritidis recovered from broiler and layer chicken farms

“…Major public health concerns include the widespread application of antibiotics in the chicken industry and the subsequent rise of multidrug-resistant ( MDR ) Salmonella strains that can be spread from animals to humans via the food chain ( Shang et al, 2018 ; Nabil et al, 2023 ). Several studies have indicated that MDR is prevalent among Salmonella serotypes isolated from animal feeds ( Holt et al, 2007 ).…”