Synergistic influence of topomimetic and chondroitin sulfate-based treatments on osteogenic potential of Ti-6Al-4V

Abstract

We combined topographical and chemical surface modifications of Ti-6Al-4V (TA6V) to improve its osteogenic potential. By acid-etching, we first generated topomimetic surface features resembling, in size and roughness, bone cavities left by osteoclasts. Next, we coated these surfaces with biomimetic Layer-by-Layer films (LbL), composed of chondroitin sulfate A and poly-l-lysine that were mechanically tuned after a post-treatment with genipin. The structural impact of each surface processing step was thoroughly inspected. The desired nano/microrough topographies of TA6V were maintained upon LbL deposition. Whereas no significant promotion of adhesion and proliferation of MC3T3-E1 preosteoblasts were detected after independent or combined modifications of the topography and the chemical composition of the substrates, osteogenic maturation was promoted when both surface treatments were combined, as was evidenced by significant long-term matrix mineralization. The results open promising route toward improved osseointegration of titanium-based implants. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1988-2000, 2016.

Properties improvement of titanium alloys scaffolds in bone tissue engineering: a literature review

“…Researchers hypothesized that PDLLA has a certain degree of inhibition of bone formation, which counteracts the positive effect of rhBMP-2. At present, the commonly used coating media commonly include chitosan, penicillin, calcium-phosphorus compounds, etc., which exhibit good cellular compatibility, no osteogenic inhibition, and could form a slow-releasing structure (70).…”

Active Osteoblasts or Quiescent Bone Lining Cells? Preosteoblasts Fate Orchestrated by Curvature and Stiffness of an In Vitro 2.5D Biomimetic Culture System

“…They are based on the "layer-by-layer" (LbL) method [14] and offer the possibility to create biomimetic thin films by judiciously selecting biomolecules present in the native ECM. In previous works [15,16,17,18] we thoroughly characterized one promising and polyvalent LbL system composed of poly-l-lysine (PLL), a cationic polypeptide known to favor cell adhesion, and chondroitin sulfate A (CSA), a natural anionic glycosaminoglycan (GAG) found in conjunctive tissues such as cartilage, skin and bone. Not only does this LbL system provide a biomimetic interface to the cells, but it also offers a nano-micrometric topography favorable to cell adhesion [16,18] and more broadly, to osseointegration.…”

“…In previous works [15,16,17,18] we thoroughly characterized one promising and polyvalent LbL system composed of poly-l-lysine (PLL), a cationic polypeptide known to favor cell adhesion, and chondroitin sulfate A (CSA), a natural anionic glycosaminoglycan (GAG) found in conjunctive tissues such as cartilage, skin and bone. Not only does this LbL system provide a biomimetic interface to the cells, but it also offers a nano-micrometric topography favorable to cell adhesion [16,18] and more broadly, to osseointegration. [19,20] In the present study, we created an easy and rapid in vitro biomimetic 2.5D microenvironment for the study of preosteoblasts behavior by controlling topographical curvatures and mechanical properties of PDMS substrates, atop which biomimetic LbL thin films were built up.…”

Proliferation und Differenzierung von mesenchymalen Stromazellen auf biofunktionalisierten Copolymer-Trägern

“…Für weitere Oberflächenoptimierungen eignen sich mehrlagige PEM. Als Polykation zeigt sich PLL als vorteilhaft, wie auch das CS als Polyanion (Labat et al 2016;Zheng et al 2021). Eine weitere Modifikation solcher PEM kann eine Implementierung von Wachstumsfaktoren sein, besonders da BMP-2 in vivo nur dann optimale Leistungen gewährleistet, wenn eine lokalisierte und kontinuierliche Freigabe des Wachstumsfaktors besteht (Macdonald et al 2011).…”