2011
DOI: 10.1074/jbc.m110.198945
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Synergistic Interaction between Leptin and Cholecystokinin in the Rat Nodose Ganglia Is Mediated by PI3K and STAT3 Signaling Pathways

Abstract: Research has shown that the synergistic interaction between vagal cholecystokinin-A receptors (CCKARs) and leptin receptors (LRbs) mediates short term satiety. We hypothesize that this synergistic interaction is mediated by cross-talk between signaling cascades used by CCKARs and LRbs, which, in turn, activates closure of K ؉ channels, leading to membrane depolar- Leptin, the product of the ob gene, is secreted primarily from white adipocyte tissue; its level in the circulation correlates with the degree of ad… Show more

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Cited by 38 publications
(46 citation statements)
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“…siRNAs for PI3K p85 alpha (SC-156021), SRC-1 (SC-36556), Erk1 (SC-156030), Erk2 (SC-156031), EGR-1 (SC-270177), and a siRNA-A unrelated to these genes used as the control (SC-37007) were diluted in Opti-MEM (Invitrogen). NG neurons at 50 -60% confluency were transfected with 15 nM of control siRNA-A, PI3K siRNA, SRC-1 siRNA, or Erk1/2 siRNA in Opti-MEM and Lipofectamine RNAiMAX (Invitrogen) for 4 -6 h as previously described (11). The neurons were incubated for 72 h with siRNA.…”
Section: Methodsmentioning
confidence: 99%
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“…siRNAs for PI3K p85 alpha (SC-156021), SRC-1 (SC-36556), Erk1 (SC-156030), Erk2 (SC-156031), EGR-1 (SC-270177), and a siRNA-A unrelated to these genes used as the control (SC-37007) were diluted in Opti-MEM (Invitrogen). NG neurons at 50 -60% confluency were transfected with 15 nM of control siRNA-A, PI3K siRNA, SRC-1 siRNA, or Erk1/2 siRNA in Opti-MEM and Lipofectamine RNAiMAX (Invitrogen) for 4 -6 h as previously described (11). The neurons were incubated for 72 h with siRNA.…”
Section: Methodsmentioning
confidence: 99%
“…siRNA was designed and constructed in lentiviral shRNA vectors to silence the expression of rat STAT3 (NM_012747), as previously described (11). In addition, an shRNA construct unrelated to these genes was constructed as a control.…”
Section: Methodsmentioning
confidence: 99%
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“…Synergistic interaction between leptin and CCK reduces short-term food intake (3,54). In vitro, leptin and CCK synergistically increase phosphorylated STAT3 production in the nodose ganglion, which contains the cell bodies of the vagal afferent neurons (20). Other studies have reported that CCK interacts with leptin at the level of the vagal afferent neurons to control the function of early growth response factor 1 (8,9).…”
mentioning
confidence: 99%