Synergistic larvicidal effect and morphological alterations induced by ethanolic extracts of Annona muricata and Piper nigrum against the dengue fever vector Aedes aegypti

Grzybowski, Adélia; Tiboni, Marcela; Silva, Mário A N; Chitolina, Rodrigo Faitta; Passos, Maurício; Fontana, José D.

doi:10.1002/ps.3409

Cited by 40 publications

(22 citation statements)

References 39 publications

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“…In addition, the larvae of the Aedes aegypti mosquito, the transmitters of dengue fever, elicited high susceptibility to the ethanolic extract of the seeds with the LC 50 of 224.27 ppm [ 127 ]. A. muricata seeds showed more than five times synergistic larvicidal activity when combined with Piper nigrum fruit ethanolic extracts ( A. muricata 90:10 P. nigrum ) [ 128 ]. The fractionation analysis of the extract showed that n -hexane is the most active fraction with an LC 50 of 73.77 ppm.…”

Section: Biological Activitiesmentioning

confidence: 99%

Annona muricata (Annonaceae): A Review of Its Traditional Uses, Isolated Acetogenins and Biological Activities

Moghadamtousi

Fadaeinasab

Nikzad

et al. 2015

IJMS

449

361

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Annona muricata is a member of the Annonaceae family and is a fruit tree with a long history of traditional use. A. muricata, also known as soursop, graviola and guanabana, is an evergreen plant that is mostly distributed in tropical and subtropical regions of the world. The fruits of A. muricata are extensively used to prepare syrups, candies, beverages, ice creams and shakes. A wide array of ethnomedicinal activities is contributed to different parts of A. muricata, and indigenous communities in Africa and South America extensively use this plant in their folk medicine. Numerous investigations have substantiated these activities, including anticancer, anticonvulsant, anti-arthritic, antiparasitic, antimalarial, hepatoprotective and antidiabetic activities. Phytochemical studies reveal that annonaceous acetogenins are the major constituents of A. muricata. More than 100 annonaceous acetogenins have been isolated from leaves, barks, seeds, roots and fruits of A. muricata. In view of the immense studies on A. muricata, this review strives to unite available information regarding its phytochemistry, traditional uses and biological activities.

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Section: Biological Activitiesmentioning

confidence: 99%

Annona muricata (Annonaceae): A Review of Its Traditional Uses, Isolated Acetogenins and Biological Activities

Moghadamtousi

Fadaeinasab

Nikzad

et al. 2015

IJMS

449

361

View full text Add to dashboard Cite

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“…Therefore, our results are consistent with other reports in the literature (LEATEMIA; LLANOS;ARANGO;GIRALDO, 2008;GRZYBOWSKI et al, 2013; (COLOM et al, 2008) and insecticidal and anti-feeding activities for Spodoptera frugiperda (J.E. Smith, 1797) larvae .…”

Section: Fungicidal and Antiaflatoxigenic Activity Of The Most Promissupporting

confidence: 93%

Exploring genetic biodiversity: secondary metabolites from Neotropical Annonaceae as a potential source of new pesticides

Ribeiro¹

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Exploring genetic biodiversity: secondary metabolites from Neotropical Annonaceae as potential source of new pesticides To investigate potential sources of novel grain protectors, this study evaluated, firstly, the bioactivity of ethanolic extracts (66) prepared from 29 species belonging to 11 different genera of Neotropical Annonaceae against the maize weevil Sitophilus zeamais (Coleoptera: Curculionidae). A screening assay demonstrated that the most pronounced effects (acute and chronic) on S. zeamais were caused by extracts from the Annona montana, A. mucosa, A. muricata and A. sylvatica seeds, and, to a lesser extent, by extracts prepared from leaves of A. montana, A. mucosa, A. muricata, and Duguetia lanceolata. However, the most active extracts (from seeds) did not affect fungal growth and aflatoxin production of Aspergillus flavus (Ascomycota: Trichocomaceae). Using the maize weevil as bioindicator, bioguided fractionations were then conducted in order to isolate, purify and characterize the possible active compound(s) from the most interesting extracts. By means of different chromatographic procedures, nine compounds (five acetogenins, three steroids, and one aromatic compound) were isolated. The acetogenins rolliniastatin-1 and ACG4 (structural determination in progress) and the aromatic compound 2,4,5trimethoxystyrene as well as the steroids campesterol, stigmasterol, and sitosterol [tested in mixture (8.44 + 12.37 + 79.19%, respectively)] showed promising grain protective properties. Furthermore, the obtained results indicate that compounds from different chemical natures have a synergistic effect on the overall biological activity of the crude extracts. In a second study, the acute and chronic toxicity of selected ethanolic seed extracts from Annona species (A. montana, A. mucosa, A. muricata, and A. sylvatica) and an acetogenin-based commercial bioinsecticide (Anosom ® 1EC) were investigated against the cabbage looper Trichoplusia ni (Lepidoptera: Noctuidae) and the green peach aphid Myzus persicae (Hemiptera: Aphididae). In the laboratory, extracts of A. mucosa and A. sylvatica as well as Anosom ® were especially active through oral and topical administration. A greenhouse trial showed that a formulated A. mucosa extract and Anosom ® were highly effective (>98% mortality) against third instar of T. ni larvae, and comparable to a pyrethrin-based commercial insecticide (Insect Spray ®) used as a positive control. Similar to results with T. ni, A. mucosa extract showed the greatest aphicidal either in laboratory or greenhouse bioassays. In a third study, the acaricidal activity [against the citrus red mite Panonychus citri (Acari: Tetranychidae)] of the ethanolic extract from A. mucosa seeds (most active) was investigated. In laboratory tests, it exhibited levels of activity superior to commercial acaricides/insecticides of natural origin [Anosom ® 1EC (annonin), Derisom ® 2EC (karanjin), and Azamax ® 1.2EC (azadirachtin + 3tigloylazadirachtol)] and similar to a synthetic acaricide [Envidor ® 24 SC (sp...

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“…The study showed that different mechanisms of the two constituents explained the observed synergism and lead to a simple and efficient phytolarvacidal formulation against Aedes aegypti mosquito. [80] Antimicrobial constituents of the essential oil obtained from fresh leaves of Zanthoxylum alatum were analyzed by TLC-direct bioautography and isolated by PLC. For assaying antifungal activity, silica gel 60 F 254 plates developed in hexane-ethyl acetate (9:1) were used for separation, and inoculum spray solution was prepared for test fungi (Alternaria alternata) with liquid potato dextrose.…”

Section: Tlc-msmentioning

confidence: 99%

Review of advances in the thin layer chromatography of pesticides: 2012–2014

Sherma

2015

Journal of Environmental Science and Health, Part B

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Publications reporting techniques and applications of thin layer chromatography (planar chromatography) for the separation, detection, qualitative, and quantitative determination, and preparative isolation of pesticides and their metabolites are reviewed for the period from November 1, 2012 to November 1, 2014. Analyses are described for a variety of sample types and pesticide classes. In addition to references on residue analysis, studies such as pesticide structure - retention relationships, identification and characterization of natural and synthesized pesticides, metabolism, bioactivity, degradation, soil mobility, and lipophilicity are covered.

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Synergistic larvicidal effect and morphological alterations induced by ethanolic extracts of Annona muricata and Piper nigrum against the dengue fever vector Aedes aegypti

Cited by 40 publications

References 39 publications

Annona muricata (Annonaceae): A Review of Its Traditional Uses, Isolated Acetogenins and Biological Activities

Annona muricata (Annonaceae): A Review of Its Traditional Uses, Isolated Acetogenins and Biological Activities

Exploring genetic biodiversity: secondary metabolites from Neotropical Annonaceae as a potential source of new pesticides

Review of advances in the thin layer chromatography of pesticides: 2012–2014

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