Synergistic or Additive Pharmacological Interactions between Magnoflorine and Cisplatin in Human Cancer Cells of Different Histological Origin

Abstract

Magnoflorine is an aporphine alkaloid present in plant species belonging to the Berberidaceae, Magnoliaceae, Menispermaceae, or Papaveraceae botanical families. The interest of magnoflorine has increased recently due to its multiplicity of pharmacological properties. The aim of this study was the analysis of combined anti-proliferative effect of magnoflorine and cisplatin and the assessment of drug–drug pharmacological interaction between these agents using isobolographic method in MDA-MB-468 human breast, NCIH1299 lung, TE671 rhabdomyosarcoma, or T98G glioblastoma cancer cell lines. Magnoflorine in combination with cisplatin at a fixed ratio of 1:1 augmented their anticancer action and yielded synergistic or additive pharmacological interactions by means of isobolographic method, therefore combined therapy using these two active agents can be a promising chemotherapy regimen in the treatment of some types of breast, lung, rhabdomyosarcoma, and glioblastoma cancers.

Magnoflorine—Isolation and the Anticancer Potential against NCI-H1299 Lung, MDA-MB-468 Breast, T98G Glioma, and TE671 Rhabdomyosarcoma Cancer Cells

“…A549, NCI-H1299, MCF7, MDA-MB-468, T98G, TE671, and HeLa cancer cells (1 × 10 4 cells/mL) [ 1 ] were plated on 96-well microplates. HSF normal cells were grown at a density of 1 × 10 5 cells/mL.…”

“…Magnoflorine (MGN) is a quaternary aporphine alkaloid widely distributed among different representatives of Magnoliaceae, Menispermaceae, Berberidaceae, or Papaveraceae species [ 1 ]. It is clear that the recent decade of scientific research brings interest back to this compound.…”

“…The published scientific papers list its vast therapeutic properties including: neuropsychopharmacological [ 2 ], anti-anxiety [ 3 , 4 ], immunomodulatory [ 5 ], anti-inflammatory [ 3 ], antioxidant [ 6 ] or antifungal [ 7 ] properties. Several studies evidenced also the anti-cancer activity of MGN [ 1 , 8 , 9 ]. Nevertheless, the mechanism of action and the influence of MGN on the progression of cancer that is of particular interest to the authors have not yet been fully understood [ 8 , 10 ].…”

Magnoflorine Ameliorates Inflammation and Fibrosis in Rats With Diabetic Nephropathy by Mediating the Stability of Lysine-Specific Demethylase 3A

“…Magnoflorine (MF), an aporphine alkaloid exists in plants belonging to the Berberidaceae, Magnoliaceae, Menispermaceae, or Papaveraceae botanical families, has been indicated to exert antioxidant, anti-inflammatory, and anti-diabetic functions ( Okon et al, 2020 ). Moreover, MF reduced the expression patterns of pro-inflammatory cytokines tumor necrosis factor alpha (TNF-α) and interleukin (IL)-1β, thus ameliorating acute lung injury induced by lipopolysaccharide ( Guo et al, 2018 ).…”

Vestibular rehabilitation after unilateral neuritis in the course of neuroborreliosis – a case report

“…One of the most popular alkaloids is magnoflorine (MGN), which is synthesized by plants from the Ranunculaceae, Menispermaceae and Magnoliaceae families [1]. MGN is a quaternary isoquinolone alkaloid, specifically an aporphyrin derivative [2,3]. These compounds are synthesized from benzylisoquinolines in the process of subtraction of two hydrogen atoms, thus creating the 9,10-dihydrophenanthrene structure from two benzene nuclei [2].…”