Synergy and mechanism of action of α-mangostin and ceftazidime against ceftazidime-resistant <i>Acinetobacter baumannii</i>

Pimchan, Thippawan; Maensiri, Duangkamol; Eumkeb, Griangsak

doi:10.1111/lam.12789

Cited by 10 publications

(6 citation statements)

References 24 publications

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“…The data were expressed as a mean ± standard error of the mean (SEM). Significant differences between these groups were examined using one-way ANOVA and Tukey's HSD post hoc test; p < 0.01 were considered to be a significant statistical difference [ 25 , 28 , 29 ].…”

Section: Methodsmentioning

confidence: 99%

The Synergy and Mode of Action of Cyperus rotundus L. Extract Plus Ampicillin against Ampicillin‐Resistant Staphylococcus aureus

Cheypratub

Leeanansaksiri

Eumkeb

2018

Evidence-Based Complementary and Alternative Medicine

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Cyperus rotundus L. has been used for pharmaceutical applications including antibacterial infections. Nevertheless, there is still no data regarding the mode of actions. This study aimed to determine the antibacterial activity and mode of actions of Cyperus rotundus extract (CRE) against ampicillin-resistant Staphylococcus aureus (ARSA) which poses a serious problem for hospitalized patients. The majority of chemical compounds of CRE were flavonoids and alkaloids. The minimum inhibitory concentrations (MICs) for ampicillin and CRE against all ARSA strains were 64 μg/ml and 0.5 mg/ml, respectively. Checkerboard assay revealed synergistic activity in the combination of ampicillin and CRE at the lowest fractional inhibitory concentration index (FICI) at 0.27. The killing curve assay had confirmed the synergistic and bactericidal activity of the combination against ARSA. Electron microscopic results showed that these ARSA cells treated with this combination caused peptidoglycan and cytoplasmic membrane (CM) damage and average cell areas significantly smaller than control. Also, this combination caused an increase in CM permeability of ARSA. CRE revealed the inhibitory activity against β-lactamase. It is normally known that some drugs are derived from flavonoids or alkaloids. So, this CRE proposes the potential to develop a novel adjunct phytopharmaceutical to ampicillin for the remedy of ARSA.

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Section: Methodsmentioning

confidence: 99%

The Synergy and Mode of Action of Cyperus rotundus L. Extract Plus Ampicillin against Ampicillin‐Resistant Staphylococcus aureus

Cheypratub

Leeanansaksiri

Eumkeb

2018

Evidence-Based Complementary and Alternative Medicine

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“…These findings are consistent with the fact that XDRAB has been increasingly isolated in clinical settings globally, which calls for combination therapy 11 . Indeed, the clinical choice of antibacterial drugs for XDRAB infections is limited, and the new therapeutic regime with combination drug use has been pursued 16,17,20,21 . Given that biofilms contribute to bacterial virulence and resistance 22 , we targeted agents with anti-biofilm property for their potential in combination drug use against A. baumannii.…”

Section: Discussionmentioning

confidence: 99%

In vitro activity of biofilm inhibitors in combination with antibacterial drugs against extensively drug-resistant Acinetobacter baumannii

Qin

Lin

Ling

2020

Sci Rep

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Acinetobacter baumannii is a common pathogen of nosocomial infection, and its ability to form biofilms further contributes to its virulence and multidrug resistance, posing a great threat to global public health. In this study, we investigated the inhibitory effects of five biofilm inhibitors (BFIs) (zinc lactate, stannous fluoride, furanone, azithromycin, and rifampicin) on biofilm formation of nine extensively drug-resistant A. baumannii (XDRAB), and assessed the synergistic antibacterial effects of these BFIs when combined with one of four conventional anti-A. baumannii antibiotics (imipenem, meropenem, tigecycline, and polymyxin B). Each of the five BFIs tested was found to be able to significantly inhibit biofilm formation of all the clinical isolates tested under sub-minimal inhibitory concentrations. Then, we observed synergistic effects (in 22%, 56% and 11% of the isolates) and additive effects (56%, 44% and 44%) when zinc lactate, stannous fluoride and furanone were combined with tigecycline, respectively. When zinc lactate and stannous fluoride were each used with a carbapenem (imipenem or meropenem), in 33% and 56–67% of the isolates, they showed synergistic and additive effects, respectively. Additivity in > 50% of the isolates was detected when rifampicin was combined with imipenem, meropenem, tigecycline, or polymyxin B; and a 100% additivity was noted with azithromycin-polymyxin B combination. However, antagonism and indifference were noted for polymyxin B in its combination with zinc lactate and stannous fluoride, respectively. In conclusion, five BFIs in combination with four antibacterial drugs showed different degrees of in vitro synergistic and additive antibacterial effects against XDRAB.

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“…Another extract, silymarin, can improve the toxicity of linezolid and synergistic anti-MRSA infection, while a high concentration silibinin with kanamycin can inhibit the growth of S. aureus. Pimchan et al (2017) demonstrated a synergistic effect between α-mangostin and ceftazidime in A. baumannii. The FICI of the combination of α-mangiferin and oxacillin against oxacillin-resistant Staphylococcus saprophyticus was 0.37.…”

Section: Review Flavonoids Combined With Antibiotics For Antibacteria...mentioning

confidence: 91%

Effects of Traditional Chinese Medicine and its Active Ingredients on Drug-Resistant Bacteria

Feng

Liu

et al. 2022

Front. Pharmacol.

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The increasing and widespread application of antibacterial drugs makes antibiotic resistance a prominent and growing concern in clinical practice. The emergence of multidrug-resistant bacteria presents a global threat. However, the development and use of novel antibacterial agents involves time-consuming and costly challenges that may lead to yet further drug resistance. More recently, researchers have turned to traditional Chinese medicine to stem the rise of antibiotic resistance in pathogens. Many studies have shown traditional Chinese medicines to have significant bacteriostatic and bactericidal effects, with the advantage of low drug resistance. Some of which when combined with antibiotics, have also demonstrated antibacterial activity by synergistic effect. Traditional Chinese medicine has a variety of active components, including flavonoids, alkaloids, phenols, and quinones, which can inhibit the growth of drug-resistant bacteria and be used in combination with a variety of antibiotics to treat various drug-resistant bacterial infections. We reviewed the interaction between the active ingredients of traditional Chinese medicines and antibiotic-resistant bacteria. At present, flavonoids and alkaloids are the active ingredients that have been most widely studied, with significant synergistic activity demonstrated when used in combination with antibiotics against drug-resistant bacteria. The reviewed studies show that traditional Chinese medicine and its active ingredients have antimicrobial activity on antibiotic-resistant bacteria, which may enhance the susceptibility of antibiotic-resistant bacteria, potentially reduce the required dosage of antibacterial agents and the rate of drug resistance. Our results provide direction for finding and developing alternative methods to counteract drug-resistant bacteria, offering a new therapeutic strategy for tackling antibiotic resistance.

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Synergy and mechanism of action of α-mangostin and ceftazidime against ceftazidime-resistant Acinetobacter baumannii

Cited by 10 publications

References 24 publications

The Synergy and Mode of Action of Cyperus rotundus L. Extract Plus Ampicillin against Ampicillin‐Resistant Staphylococcus aureus

The Synergy and Mode of Action of Cyperus rotundus L. Extract Plus Ampicillin against Ampicillin‐Resistant Staphylococcus aureus

In vitro activity of biofilm inhibitors in combination with antibacterial drugs against extensively drug-resistant Acinetobacter baumannii

Effects of Traditional Chinese Medicine and its Active Ingredients on Drug-Resistant Bacteria

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