Synergy Between Ruminal Fibrolytic Enzymes and Enzymes from Trichoderma Longibrachiatum

Morgavi, Diego P.; Beauchemin, K. A.; Nsereko, V. L.; Rode, L. M.; Iwaasa, A. D.; Yang, Wenzhu; McAllister, Tim A.; Wang, Y.

doi:10.3168/jds.s0022-0302(00)74997-6

Cited by 145 publications

(110 citation statements)

References 25 publications

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“…Pesquisas têm mostrado que a suplementação com enzimas exógenas (celulase e hemicelulase) promove o aumento da atividade da celulase e xilanase no rúmen (Hristov et al, 2000;Morgavi et al, 2000). Krause et al (1998) verificaram que a adição de enzimas fibrolíticas à dieta alterou a composição química do Tabela 4 -Coeficientes de digestibilidade total da MS (DMS), MO (DMO), PB (DPB), FDN (DFDN), FDA (DFDA), CEL (DCEL) e HEM (DHEM) das dietas experimentais Table 4 volumoso, reduzindo os teores de FDN e FDA.…”

Section: Resultsunclassified

“…, avaliando dietas compostas de silagem de cevada e cevada em grão, verificaram aumento de 58,8 para 61,7% na digestibilidade total da FDN com a adição de enzimas, mas não notaram efeito sobre o consumo e a digestão ruminal dos nutrientes. Morgavi et al (2000), avaliando o efeito da combinação de enzimas microbianas e extraídas do fungo Trichoderma longibrachiatum na degradação da silagem de milho, observaram que a complexidade da estrutura química da silagem de milho causou variações nas respostas com a combinação destas misturas enzimáticas. Segundo esses autores, a celulase seria mais limitante na degradação da fibra da silagem de milho que a xilanase.…”

Section: Resultsunclassified

“…Além disso, segundo Morgavi et al (2000), a formulação dos complexos enzimáticos comerciais é normalmente padroniTabela 5 -Efeito da interação fontes de volumoso × níveis de enzima sobre a digestibilidade aparente total da EB (DEB) …”

Section: Fonte De Volumoso Médiaunclassified

“…Schingoethe et al (1999) observaram aumento na produção de leite (10,8%) e no teor de gordura e proteína do leite (20 e 13%, respectivamente) com a adição de enzimas fibrolíticas (celulase e xilanase) em dietas contendo silagem de milho. Embora as formulações de complexos enzimáticos, como os extraídos do fungo Trichoderma spp., melhorem a digestão dos alimentos em animais ruminantes (Morgavi et al, 2000), Firkins et al (1990) Foram testadas as seguintes dietas: SMSE -silagem de milho sem adição de enzimas fibrolíticas; SMCE -silagem de milho com a adição de enzimas fibrolíticas; FESE -feno de tifton 85 sem a adição de enzimas fibrolíticas; FECE -feno de tifton 85 com a adição de enzimas fibrolíticas.…”

Section: Introductionunclassified

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Consumo e digestibilidade aparente total em bovinos sob suplementação com enzimas fibrolíticas

Martins¹,

Vieira

Berchielli

et al. 2006

R. Bras. Zootec.

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RESUMO -Avaliou-se o efeito da suplementação com enzimas fibrolíticas (celulase e xilanase) sobre o consumo e a digestibilidade aparente total dos nutrientes de dietas compostas de silagem de milho e feno de tifton 85 (Cynodon spp.) cortado aos 90 dias. Oito bovinos (340 kg de PV) fistulados no rúmen foram distribuídos em dois quadrados latinos 4 x 4, em esquema fatorial 2 x 2 (fonte de volumoso e adição ou não de enzimas). O complexo enzimático testado foi proveniente de fonte comercial e extraído dos fungos Aspergillus niger e Trichoderma longibrachiatum, sendo fornecido na quantidade de 12 g/ animal/dia, misturado à ração total. Não houve efeito da suplementação enzimática sobre o consumo de nutrientes para ambos os volumosos, porém, a adição de enzimas aumentou a digestibilidade total de FDN, FDA e CEL de 36,87; 36,21 e 46,89%, respectivamente, para 41,19; 40,01 e 50,46%, respectivamente.Palavras-chave: celulase, feno de tifton, ruminantes, silagem de milho, xilanase Intake and apparent total tract digestibility in cattle supplemented with fibrolytic enzymes ABSTRACT -The objective of this trial was to evaluate the effect of feeding fibrolytic enzyme (cellulose and xylanase) on intake and apparent total tract digestibility of nutrients in cattle receiving diets containing corn silage or Tifton 85 hay (Cynodon spp.). Eight animals fitted with ruminal cannulae and averaging 340 kg of body weight were randomly assigned to two replicated 4 x 4 Latin squares in a 2 x 2 factorial arrangement of treatments (forage sources with or without fibrolytic enzyme supplementation). The commercial enzymatic complex used, which was extracted from Aspergillus niger and Trichoderma longibrachiatum fungi was mixed with the forage and concentrate and supplied at a daily amount of 12 g/animal. There was no effect of enzymatic supplementation on nutrient intake for both forage sources. However, the addition of fibrolytic enzymes increased the apparent total tract digestibilities of neutral detergent fiber (NDF), acid detergent fiber (ADF) and cellulose from 36.87, 36.21, and 46.89% to 41.19, 40.01, and 50.46%, respectively. Key Words: cellulase, corn silage, ruminants, tifton hay, xylanase IntroduçãoNos sistemas de produção de ruminantes, grande parte (40 a 90%) das exigências nutricionais é atendida por meio de volumosos e, em algumas regiões do país, os rebanhos destinados à produção de carne e leite dependem de forragens conservadas para o suprimento de nutrientes durante os períodos de escassez de alimentos.Segundo Van Soest (1965), o consumo de alimentos é inversamente relacionado ao teor de FDN em dietas com mais de 60% deste nutriente, de modo que a parede celular é o constituinte da planta que possui maior relação com o consumo voluntário.O aproveitamento de alimentos fibrosos pelos ruminantes está relacionado à síntese e secreção de enzimas pelos microrganismos do rúmen, promovendo a hidrólise da parede celular das plantas. Entretanto, a conversão dos alimentos, especialmente os fibrosos, para produção de carne e leite ...

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Section: Resultsunclassified

Section: Fonte De Volumoso Médiaunclassified

Section: Introductionunclassified

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Consumo e digestibilidade aparente total em bovinos sob suplementação com enzimas fibrolíticas

Martins¹,

Vieira

Berchielli

et al. 2006

R. Bras. Zootec.

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“…Exogenous enzymes are most effective when applied to feed prior to ingestion by cattle, as explained by Beauchemin Morgavi et al (2000a)]. The graph shows the hydrolysis of corn silage incubated at pH 6.0 in the presence of ruminal and Trichoderma enzymes.…”

Section: Pre-ingestive Effectsmentioning

confidence: 99%

Mode of action of exogenous cell wall degrading enzymes for ruminants

Beauchemin¹,

Colombatto²,

Morgavi³

et al. 2004

Can. J. Anim. Sci.

121

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Rode, L. M. 2004. Mode of action of exogenous cell wall degrading enzymes for ruminants. Can. J. Anim. Sci. 84: [13][14][15][16][17][18][19][20][21][22]. Recent studies have shown that adding exogenous fibrolytic enzymes to ruminant diets can increase milk production of dairy cows and weight gain of growing beef cattle as a result of enhanced feed digestion. While much progress has been made in terms of advancing feed enzyme technology for ruminants, considerable research is still required to develop more effective enzyme products. The mode of action whereby exogenous enzymes improve digestion of plant cell wall is complex, and there is evidence for numerous potential modes of action suggesting they are interdependant. A mode of action that accounts for the most critical factors that explain the observed increases in feed digestion is presented. Adding exogenous enzymes to the diet increases the hydrolytic capacity of the rumen mainly due to increased bacterial attachment, stimulation of rumen microbial populations and synergistic effects with hydrolases of ruminal microorganisms. The net effect is increased enzymic activity within the rumen, which enhances digestibility of the total diet fed. Thus, improvements in digestibility are not limited to the dietary component to which the enzymes are applied, which explains why fibrolytic enzymes can be effective when added to supplement or grain. The magnitude of the improvements in feed digestibility reported in some research studies using feed enzymes suggests a viable future for enzyme products in commercial ruminant production systems. A more complete understanding of the mode of action of these products will allow development of low-cost enzyme products designed specifically to improve feed digestion by ruminants. Des études récentes montrent que l'addition d'enzymes fibrolytiques exogènes à la ration des ruminants accroît la production des vaches laitières et le poids gagné par les bovins de boucherie à cause d'une meilleure digestion des aliments. Bien que la technologie des enzymes alimentaires destinés aux ruminants ait progressé, il reste encore beaucoup à faire avant qu'on arrive à des produits enzymatiques plus efficaces. La manière dont les enzymes exogènes facilitent la digestion de la paroi cellulosique est particulièrement complexe et tout indique que les nombreux modes d'action potentiels sont interdépendants. Les auteurs proposent un mode d'action qui tient compte des principaux facteurs expliquant la meilleure digestion des aliments observée. L'addition d'enzymes exogènes à la ration accroît la capacité d'hydrolyse du rumen principalement à cause d'une meilleure fixation des bactéries, d'une stimulation de la microflore du rumen et de la synergie avec les hydrolases des microorganismes. Il s'ensuit une plus grande activité enzymatique dans le rumen, ce qui améliore la digestibilité de la ration. Cette meilleure digestibilité ne vient donc pas uniquement de la composante alimentaire à laquelle s'attaquent les enzymes, ce qui explique l'efficacité de...

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Enhanced enzymatic hydrolysis of corn stover using twin‐screw extrusion under mild conditions

Elalami

Aouine

Monlau³

et al. 2022

Biofuels Bioprod Bioref

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This paper aims to investigate the effect of extrusion at high solid loading on corn stover (CS) properties and its enzymatic hydrolysis. This biomass was extruded under different screw speeds and different solid loadings and the impact of these parameters on the physicochemical properties was evaluated. It was found that lignocellulosic components were not significantly affected by the pretreatment, while the surface area increased with solid loading and rotation speed. Different enzyme cocktails were used for the enzymatic hydrolysis of extruded and untreated CS. Overall, mild twin‐extrusion enhanced the enzymatic hydrolysis of CS through an increase in glucose and xylose yields by 134–212% and 214–294%, respectively, when using Trichoderma longibrachiatum cellulase. The highest sugar content was obtained from CS extruded under 400 g total solids per liter and 200 rpm. The energy efficiency of the pretreatment was also assessed and was found to be maximal at 400 g total solids per liter and 200 rpm. © 2022 Society of Chemical Industry and John Wiley & Sons, Ltd.

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Synergy Between Ruminal Fibrolytic Enzymes and Enzymes from Trichoderma Longibrachiatum

Cited by 145 publications

References 25 publications

Consumo e digestibilidade aparente total em bovinos sob suplementação com enzimas fibrolíticas

Consumo e digestibilidade aparente total em bovinos sob suplementação com enzimas fibrolíticas

Mode of action of exogenous cell wall degrading enzymes for ruminants

Enhanced enzymatic hydrolysis of corn stover using twin‐screw extrusion under mild conditions

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