Synergy effects of copper and L-arginine on osteogenic, angiogenic, and antibacterial activities

Noori, Alireza; Hoseinpour, Mahdieh; Kolivand, Sedighe; Lotfibakhshaiesh, Nasrin; Azami, Mahmoud; Ai, Jafar; Ebrahimi‐Barough, Somayeh

doi:10.1016/j.tice.2022.101849

Cited by 13 publications

(5 citation statements)

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“…Our previous research indicated that copper at concentrations greater than 2.5 mM possessed antibacterial properties but was also toxic to eukaryote cells 12 . However, this study found different results.…”

Section: Resultsmentioning

confidence: 99%

“…Copper's antibacterial impact is thought to be caused by multiple mechanisms, including the disruption of bacterial membrane integrity, the generation of free radicals, and the alteration of bacterial protein and enzyme structure 11 . It is critical to remember that an increased concentration of copper may cause toxicity in human cells; thus, the concentration of copper must be optimized to precisely target bacteria while not hurting human cells 12 . In this context, Chengtie Wu and colleagues found that adding copper ions to bioactive glass increased the expression of genes involved in osteogenesis and blood vessel formation.…”

Section: Introductionmentioning

confidence: 99%

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Exploring the various effects of Cu doping in hydroxyapatite nanoparticle

Noori,

Hoseinpour,

Kolivand

et al. 2024

Sci Rep

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Adding foreign ions to hydroxyapatite (HAp) is a popular approach for improving its properties. This study focuses on the effects of calcium substitution with copper in HAp. Instead of calcium, copper ions were doped into the structure of hydroxyapatite nanoparticles at 1%, 3%, and 5% concentrations. XRD analysis showed that the amount of substituted copper was less than needed to generate a distinct phase, yet its lattice parameters and crystallinity slightly decreased. Further, the results of degradation tests revealed that copper doping in hydroxyapatite doubled calcium ion release in water. The incorporation of copper into the apatite structure also boosted the HAp zeta potential and FBS protein adsorption onto powders. According to antibacterial investigations, a concentration of 200 mg/ml of hydroxyapatite containing 5% copper was sufficient to effectively eradicate E. coli and S. aureus bacteria. Furthermore, copper improved hydroxyapatite biocompatibility. Alkaline phosphatase activity and alizarin red tests showed that copper in hydroxyapatite did not inhibit stem cell differentiation into osteoblasts. Also, the scratch test demonstrated that copper-containing hydroxyapatite extract increased HUVEC cell migration. Overall, our findings demonstrated the utility of incorporating copper into the structure of hydroxyapatite from several perspectives, including the induction of antibacterial characteristics, biocompatibility, and angiogenesis.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Exploring the various effects of Cu doping in hydroxyapatite nanoparticle

Noori,

Hoseinpour,

Kolivand

et al. 2024

Sci Rep

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“…Fish bones as a source of HA are supplemented with L-arginine protein (L-Arg) to promote the development of apatite in PMMA bone cement. L-Arg is a semi-essential amino acid required for various biological processes, including osteogenic and angiogenic activities [ 21 , 22 ]. It is a crucial building block for the production of proteins and other compounds such as nitric oxide (NO), ornithine, proline and polyamines [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

“…NO has been shown to promote bone formation and limit bone resorption. Therefore, L-Arg contributes to angiogenesis, which is necessary for bone remodelling and fracture healing [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

PMMA bone cement with L -arginine/nano fish bone nanocomplex for apatite formation

Khoman,

Kalijaga,

Aisah

et al. 2024

R. Soc. Open Sci.

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Bone cement is one of the materials used in orthopaedics that serves various functions, such as binding bone implants, replacing damaged bones and filling spaces within bones. Various materials have been used to synthesize bone cement, and one promising material for further research is fish bone waste-based bone cement. This study investigates the potential of fish bone waste-based bone cement by incorporating nano fish bone (NFB) and L -arginine ( L -Arg) protein into polymethyl methacrylate (PMMA) to examine apatite growth. NFB derived from the Salmo salar fish positively influences osteoblast cell proliferation and differentiation, while L-Arg enhances biocompatibility and antibiotic properties. The NFB/L-Arg combination holds promise in accelerating new bone formation and cell growth, both of which are crucial for fracture healing and bone remodelling. Tensile strength tests reveal the superior performance of BC-PMMA-1-NFB/L-Arg (36.11 MPa) compared with commercial PMMA (32 MPa). Immersion tests with simulated body fluid (SBF) solution for 7 days reveal accelerated apatite layer formation, emphasizing the potential benefits of NFB/ L -Arg in bone cement applications.

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“…Arginine is a semi-essential amino acid in the diet, as well as a naturally occurring amino acid in the body [1]. It is especially abundant in meats and nuts.…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of silicon dioxide nanoparticles and L arginine@silicon dioxide nano- composites using cellulose of Zizyphus Spina-Christi along with biological evaluation

Mojaddami¹,

Chamkouri²,

Chamkouri³

et al. 2022

Bull. Chem. Soc. Eth.

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ABSTRACT. The recent research focused on the green synthesis of silicon dioxide nanoparticles, SiO2@Cellulose of Zizyphus Spina-Christi nanocomposites, and L-Arginine@SiO2@Cellulose of Zizyphus Spina-Christi nanocomposites using cellulose of Zizyphus Spina-Christi as a new green polymeric surfactant. The structures of nanoparticles and nanocomposites were characterized by different spectroscopy and microscopy techniques. Nanoparticles and nanocomposites were utilized to determine the concentration of chromium, cadmium, and lead in COVID-19 patients using double-vortex-ultrasonic assisted surfactant enhanced dispersive liquid-liquid microextraction. Mean recoveries of chromium, cadmium, and lead were obtained in the range of 86-98% with relative standard deviations below 4%. The advantages of the proposed method are green and novel polymer surfactant with low detection limit. Finally, antibacterial activities were investigated. The maximum inhibition zone of L-Arginine@SiO2@Cellulose of Zizyphus Spina-Christi nanocomposites was obtained for Staphylococcus Aureus (21.9±0.4 mm). L-Arginine@SiO2@Cellulose of Zizyphus Spina-Christi nanocomposites have low cytotoxicity against MCF-7 cancer cells. These results indicated the potential ability of L-Arginine@SiO2@Cellulose of Zizyphus Spina-Christi nanocomposites in the determination of metal concentrations in biological samples along with good antibacterial properties and cytotoxic properties. KEY WORDS: Green synthesis, Cellulose of Zizyphus Spina-Christi, Silicon dioxide nanoparticles, COVID-19, Biological activity Bull. Chem. Soc. Ethiop. 2023, 37(2), 265-276. DOI: https://dx.doi.org/10.4314/bcse.v37i2.2

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Synergy effects of copper and L-arginine on osteogenic, angiogenic, and antibacterial activities

Cited by 13 publications

References 46 publications

Exploring the various effects of Cu doping in hydroxyapatite nanoparticle

Exploring the various effects of Cu doping in hydroxyapatite nanoparticle

PMMA bone cement with L -arginine/nano fish bone nanocomplex for apatite formation

Green synthesis of silicon dioxide nanoparticles and L arginine@silicon dioxide nano- composites using cellulose of Zizyphus Spina-Christi along with biological evaluation

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