Synovial fluid analysis

Brannan, Scott R.; Jerrard, David A.

doi:10.1016/j.jemermed.2005.05.029

Cited by 110 publications

(83 citation statements)

References 54 publications

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“…In the literature, the overall sensitivity ranges between 30 and 60 % [1,3,7], whereas for prosthetic joint infections, Morgan et al identified a 27 % sensitivity of intraoperative Gram staining [17], while the value of inflammatory markers for predicting culture-positive arthritis was less convincing. Several studies mentioned a positive association between occurrence of septic arthritis and elevated serum CRP levels [17].…”

Section: Discussionmentioning

confidence: 99%

“…These analyses were performed by using as outcome bacterial detection by Gram stain (Table 3) or alternatively by microbiological culture (Table 4). In multivariate analyses, several host parameters were significantly linked with positive Gram stain results, in particular steroid medication, CRP levels>150 mg/l and synovial leukocyte counts > 180,000 cells/mm 3 . In contrast, other parameters such as the presence of underlying prosthetic joints, the size of articulation, gender, ongoing antibiotic therapy or the presence of crystals were not significantly linked with bacterial detection by Gram staining (Table 3).…”

Section: Logistic Regression Analysesmentioning

confidence: 99%

“…Besides evaluating the usefulness of these staining procedures for daily clinical practice, we tried to identify clinical variables that might help to predict positive stain results. In contrast, detailed therapeutic and pathophysiological aspects [3,19] were not addressed in this study.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, medical and surgical teams frequently await the results of synovial fluid cultures for the definitive diagnosis of septic arthritis, in the presence of clinical signs and purulent synovial fluid in joint aspirates. As cartilage damage is believed to occur within a few days in most non-gonococcal arthritis cases, many surgeons rapidly drain and start antibiotic treatment before receiving the microbiological culture results [1,2], unless there is a strong suspicion of recurrent gout or pyrophosphate-related joint inflammation [3,4].…”

Section: Introductionmentioning

confidence: 99%

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Gram and acridine orange staining for diagnosis of septic arthritis in different patient populations

Cunningham

Seghrouchni

Ruffieux

et al. 2014

International Orthopaedics (SICOT)

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Section: Discussionmentioning

confidence: 99%

Section: Logistic Regression Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Gram and acridine orange staining for diagnosis of septic arthritis in different patient populations

Cunningham

Seghrouchni

Ruffieux

et al. 2014

International Orthopaedics (SICOT)

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“…These findings are especially important in conditions characterized by abnormal glycemic regulation, namely in diabetes mellitus (DM) patients, where significant variations in glycemia and synovial fluid glucose concentration frequently occur [Brannan and Jerrard, 2006]. In those circumstances, high extracellular glucose concentrations may counteract TGF effects and eventually those of other anabolic growth factors, such as IGF [Kelley et al, 1999], suggesting that hyperglycemia may be an initiating and/or aggravating factor linking DM to OA.…”

Section: Discussionmentioning

confidence: 99%

Role of glucose as a modulator of anabolic and catabolic gene expression in normal and osteoarthritic human chondrocytes

et al. 2011

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Cartilage matrix homeostasis involves a dynamic balance between numerous signals that modulate chondrocyte functions. This study aimed at elucidating the role of the extracellular glucose concentration in modulating anabolic and catabolic gene expression in normal and osteoarthritic (OA) human chondrocytes and its ability to modify the gene expression responses induced by pro-anabolic stimuli, namely Transforming Growth Factor-b (TGF). For this, we analyzed by real time RT-PCR the expression of articular cartilage matrix-specific and nonspecific genes, namely collagen types II and I, respectively. The expression of the matrix metalloproteinases (MMPs)-1 and -13, which plays a major role in cartilage degradation in arthritic conditions, and of their tissue inhibitors (TIMP) was also measured. The results showed that exposure to high glucose (30 mM) increased the mRNA levels of both MMPs in OA chondrocytes, whereas in normal ones only MMP-1 increased. Collagen II mRNA was similarly increased in normal and OA chondrocytes, but the increase lasted longer in the later. Exposure to high glucose for 24 h prevented TGF-induced downregulation of MMP-13 gene expression in normal and OA chondrocytes, while the inhibitory effect of TGF on MMP-1 expression was only partially reduced. Other responses were not significantly modified. In conclusion, exposure of human chondrocytes to high glucose, as occurs in vivo in diabetes mellitus patients and in vitro for the production of engineered cartilage, favors the chondrocyte catabolic program. This may promote articular cartilage degradation, facilitating OA development and/or progression, as well as compromise the quality and consequent in vivo efficacy of tissue engineered cartilage. J. Cell. Biochem. 112: 2813Biochem. 112: -2824Biochem. 112: , 2011. ß 2011 Wiley-Liss, Inc. KEY WORDS: COLLAGEN; GENE EXPRESSION; GLUCOSE; HUMAN CHONDROCYTE; MMP; OSTEOARTHRITIS; TIMPA rticular cartilage is a specialized connective tissue that supports and distributes loads and ensures a near-frictionless motion in joints. These unique properties are due to the structural organization of the main macromolecules that compose the cartilage extracellular matrix, namely collagens and proteoglycans. Chondrocytes, the only cell type present in articular cartilage, are embedded in the extracellular matrix and are responsible for maintaining its homeostasis by ensuring the synthesis and turnover of its components [Martel-Pelletier et al., 2008;Goldring and Marcu, 2009].Cartilage matrix homeostasis involves a dynamic balance between a variety of signals that modulate chondrocyte functions, namely mechanical forces, cytokines and growth factors and cellmatrix interactions, some favoring an anabolic program and others stimulating catabolic responses. Aging and mechanical stress of joints are major risk factors for osteoarthritis (OA), but growing evidence indicates that metabolic factors play an important role in disease development and progression. For instance, a significant positive correlation wa...

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