Synovial Fluid Cytokines, Chemokines and MMP Levels in Osteoarthritis Patients with Knee Pain Display a Profile Similar to Many Rheumatoid Arthritis Patients

Meehan, Richard T.; Regan, Elizabeth A.; Hoffman, E; Wolf, Molly L.; Gill, Mary; Crooks, James; Parmar, Prashant J.; Scheuring, Richard A.; Hill, John C.; Pacheco, Karin; Knight, Vijaya

doi:10.3390/jcm10215027

Cited by 24 publications

(22 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The elevated levels of IL-1β, IL-6, and TNF-α, as well as of MMPs, found in the SF of both OA and RA patients confirmed their important role in the pathogenesis of these joint diseases [ 12 , 32 ]. Therefore, an excess of these pro-inflammatory mediators and enzymes in SF can affect chondrocyte homeostasis and induce the production of other cytokines and matrix-degrading enzymes; in addition, they also inhibit the synthesis of proteoglycans and type II collagen, thereby causing cartilage degradation in OA and RA [ 8 , 32 ].…”

Section: Discussionmentioning

confidence: 93%

“…SF is mainly involved in the maintenance and health of the articular cartilage. It has been observed that chondrocyte metabolism is deeply influenced by the specific composition of SF [ 12 , 14 ]. This evidence stimulated an increasing interest in comprehending the exact effect of the SF microenvironment in cartilage homeostasis.…”

Section: Discussionmentioning

confidence: 99%

“…Inflammatory and degenerative processes occurring in OA and RA induced modification in the molecular and cellular composition of SF. Indeed, in these conditions, it becomes less viscous and loses its unique viscoelastic properties due to a reduction in the molecular weight and concentration of hyaluronic acid (HA), as well as due to changes in cytokines, enzymes profiles, and inflammatory white blood cells (WBCs) concentrations [ 10 , 11 , 12 , 13 , 14 ]. In particular, in RA and OA, the SF is characterized by an increased content of inflammatory mediators (prostaglandin E2, leukotriene B4), cytokines (interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α), nitric oxide (NO), and reactive oxygen species (ROS), released from chondrocytes, synoviocytes, and WBCs, which contributes to articular cartilage degradation [ 3 , 5 , 8 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Synovial Fluid Regulates the Gene Expression of a Pattern of microRNA via the NF-κB Pathway: An In Vitro Study on Human Osteoarthritic Chondrocytes

Cheleschi

Tenti

Lorenzini

et al. 2022

IJMS

View full text Add to dashboard Cite

Synovial fluid (SF) represents the primary source of nutrients of articular cartilage and is implicated in maintaining cartilage metabolism. We investigated the effects of SF, from patients with osteoarthritis (OA), rheumatoid arthritis (RA), and controls, on a pattern of microRNA (miRNA) in human OA chondrocytes. Cells were stimulated with 50% or 100% SF for 24 h and 48 h. Apoptosis and superoxide anion production were detected by cytometry; miRNA (34a, 146a, 155, 181a), cytokines, metalloproteinases (MMPs), type II collagen (Col2a1), antioxidant enzymes, B-cell lymphoma (BCL)2, and nuclear factor (NF)-κB by real-time PCR. The implication of the NF-κB pathway was assessed by the use of NF-κB inhibitor (BAY-11-7082). RA and OA SF up-regulated miR-34a, -146a, -155, -181a, interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, MMP-1, MMP-13, and ADAMTs-5 gene expression, while it down-regulated Col2a1. Pathological SF also induced apoptosis, reduced viability, and decreased BCL2 mRNA, whereas it increased superoxide anions, the expression of antioxidant enzymes, p65 and p50 NF-κB. Opposite and positive results were obtained with 100% control SF. Pre-incubation with BAY-11-7082 counteracted SF effects on miRNA. We highlight the role of the SF microenvironment in regulating some miRNA involved in inflammation and cartilage degradation during OA and RA, via the NF-κB pathway.

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Synovial Fluid Regulates the Gene Expression of a Pattern of microRNA via the NF-κB Pathway: An In Vitro Study on Human Osteoarthritic Chondrocytes

Cheleschi

Tenti

Lorenzini

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…By comparing 16 biomarkers in the SF of patients with OA and RA, the authors showed that the level of only six biomarkers was significantly higher in SF than active RA compared to OA: TNF-α, IL-1-β IL-7, MMP-1, MMP-2 and MMP-3. The level of MMP- 9 in SF did not differ in patients with OA and RA [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Matrix Metalloproteinase-9 Level in Synovial Fluid—Association with Joint Destruction in Early Rheumatoid Arthritis

et al. 2023

View full text Add to dashboard Cite

Background and objective: Matrix metalloproteinases (MMPs) are the key enzymes in the pathogenesis of cartilage and joint damage and potentially a new biomarker of the early erosive form of rheumatoid arthritis (RA). Firstly, the study aimed to compare the level of MMP-9 in plasma (PL) and synovial fluid (SF) of patients with RA and osteoarthritis (OA). Secondly, the goal was to examine the association of MMP-9 level in PL and SF with early erosive changes in RA, and finally, to determine the association of MMP-9 level with serological parameters of the disease (rheumatoid factor-RF and anti-citrulline protein antibodies-ACPA). Materials and Methods: A total of 156 subjects were involved in this study (84 patients with RA and 72 patients with OA, who were involved as a control group). MMP-9 level was measured in PL and SF of all subjects by the sandwich enzyme-linked immunosorbent assay (ELISA) method. Standard radiographs of the hands and feet were used to detect joint damage and classification into erosive or non-erosive RA. The Larsen score (LS) was used for the quantitative assessment of joint damage, and its annual change (∆ LS) was used to assess the radiographic progression of the disease. Results: MMP-9 level in PL and SF was significantly higher in RA compared to controls (PL: 19.26 ± 7.54 vs. 14.57 ± 3.11 ng/mL, p< 0.01; SF: 16.17 ± 12.25 vs. 0.75 ± 0.53 ng/mL, p < 0.001) as well as in SF of patients with erosive compared to non-erosive RA (18.43 ± 12.87 vs. 9.36 ± 7.72; p < 0.05). Faster radiographic progression was recorded in erosive compared to non-erosive early RA (11.14 ± 4.75 vs. 6.13 ± 2.72; p < 0.01). MMP-9 level in SF, but not in PL, significantly correlates with the radiographic progression in both erosive and non-erosive RA (ρ = 0.38 and ρ = 0.27). We did not find a significant association between RF and MMP-9 level in early RA, but the ACPA level significantly correlates with MMP-9 level in SF (r = 0.48). Conclusion: The level of MMP-9 in plasma and synovial fluid of patients with RA is significantly higher compared to patients with osteoarthritis. The level of MMP-9 in synovial fluid is significantly higher in erosive than non-erosive early RA. It is significantly associated with the radiographic progression of the disease and the level of anti-citrulline protein antibodies.

show abstract

“…The main pro-inflammatory cytokines in the pathogenesis of OA are interleukin-1β (IL-1β)—associated with cartilage degradation, tumor necrosis factor α (TNFα)—controlling the cascade of inflammatory processes and IL-6—one of the main regulatory cytokines in inflammatory reactions in the body. IL-1β and TNFα are produced by chondrocytes, osteoblasts, synovial fibroblasts and mononuclear cells (lymphocytes, monocytes) [ 78 ]. These cytokines regulate the production of other pro-inflammatory and catabolic factors.…”

Section: Potential Soluble Biomarkers Of Osteoarthritismentioning

confidence: 99%

Soluble and EV-Associated Diagnostic and Prognostic Biomarkers in Knee Osteoarthritis Pathology and Detection

Morávek

Matejová

Špaková

2023

Life

View full text Add to dashboard Cite

Osteoarthritis (OA) is the most common degenerative disease of the connective tissue of the human musculoskeletal system. Despite its widespread prevalence, there are many limitations in its diagnosis and treatment. OA diagnosis currently relies on the presence of clinical symptoms, sometimes accompanied by changes in joint X-rays or MRIs. Biomarkers help not only to diagnose early disease progression but also to understand the process of OA in many ways. In this article, we briefly summarize information on articular joints and joint tissues, the pathogenesis of OA and review the literature about biomarkers in the field of OA, specifically inflammatory cytokines/chemokines, proteins, miRNA, and metabolic biomarkers found in the blood, synovial fluid and in extracellular vesicles.

show abstract

Synovial Fluid Cytokines, Chemokines and MMP Levels in Osteoarthritis Patients with Knee Pain Display a Profile Similar to Many Rheumatoid Arthritis Patients

Cited by 24 publications

References 38 publications

Synovial Fluid Regulates the Gene Expression of a Pattern of microRNA via the NF-κB Pathway: An In Vitro Study on Human Osteoarthritic Chondrocytes

Synovial Fluid Regulates the Gene Expression of a Pattern of microRNA via the NF-κB Pathway: An In Vitro Study on Human Osteoarthritic Chondrocytes

Matrix Metalloproteinase-9 Level in Synovial Fluid—Association with Joint Destruction in Early Rheumatoid Arthritis

Soluble and EV-Associated Diagnostic and Prognostic Biomarkers in Knee Osteoarthritis Pathology and Detection

Contact Info

Product

Resources

About