Synovial fluid proteins are required for the induction of interleukin-1β production by monosodium urate crystals

Scanu, Anna; Oliviero, Francesca; Gruaz, Lyssia; Galozzi, Paola; Luisetto, Roberto; Ramonda, Roberta; Burger, Danielle; Punzi, Leonardo

doi:10.3109/03009742.2015.1124452

Cited by 14 publications

(12 citation statements)

References 35 publications

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“…Additionally, the IL-1β level in the synovial membrane was positively correlated with OA grade, and joint space width was negatively correlated with joint activity (31,32). Additionally, IL-1β was detected in human OA cartilage, especially in early stage OA and the IL-1β level in the synovial fluid was correlated with synovial fluid uric acid level in patients with KOA; thus, it was concluded that synovial fluid uric acid could be a danger signal that contributes to increasing KOA risk through NLRP3-mediated inflammasome (33,34).…”

Section: Discussionmentioning

confidence: 98%

Differential miRNAomics of the synovial membrane in knee osteoarthritis induced by bilateral anterior cruciate ligament transection in rats

Zhou

Zhao

et al. 2018

Mol Med Report

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The differential microRNA (miRNA) omics of the synovial membrane were investigated using a rat model of knee osteoarthritis (KOA) induced by bilateral anterior cruciate ligament transection, which produced pathological biomarkers in KOA. Sprague‑Dawley rats were randomly divided into two groups; Sham‑operated and KOA‑operated group. The KOA rats were subjected to bilateral anterior cruciate ligament transection. After 6 weeks, total RNA was extracted from the knee joint synovial membrane of the rats and a microRNA (miR) microarray was performed to identify differentially expressed miRs. Subsequently, the obtained differentially expressed miRs were validated by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis. A total of 24 miRs were identified with alterations ≥1.5‑fold in the synovial membrane in the KOA‑operated group compared with the sham‑operated group, of which 4 miRs (miR‑532‑5p, ‑200b‑5p, ‑377‑3p and ‑759‑5p) were decreased and 20 miRs (miR‑382‑3p, ‑223‑3p, ‑100‑5p, ‑30d‑5p, ‑183‑5p, ‑130, ‑92b‑3p, ‑125b‑3p, ‑151‑3p, ‑155‑3p, 27a‑3p, ‑146b‑3p, ‑885‑5p, ‑352, ‑184, ‑345‑5p, ‑30a‑5p and ‑9a‑5p) were increased. Subsequently, RT‑qPCR was used to validate the expressions of miR‑223, ‑100, ‑345, ‑130, ‑382, ‑377, ‑352, ‑200b, ‑9a and ‑183, which were upregulated by a fold change of ≥1.5 in synovial membranes of KOA rats compared with shams. Furthermore, in vitro miR‑223 mimic could suppress the luciferase activity of NACHT, LRR and PYD domains‑containing protein 3 (NLRP3) 3' untranslated region by detecting of dual luciferase reporter vector. Additionally, the expression of NLRP3, interleukin (IL)‑1β and IL‑18 significantly increased in the synovial membrane of KOA rats. A total of 24 different miRs were determined by comparing the miRNAomics in the synovial membrane of the KOA model rats. Furthermore, the miR‑233‑regulated NLRP3 inflammasome was implicated in synovial membrane injury, which may be an important mechanism of KOA pathogenesis.

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Section: Discussionmentioning

confidence: 98%

Differential miRNAomics of the synovial membrane in knee osteoarthritis induced by bilateral anterior cruciate ligament transection in rats

Zhou

Zhao

et al. 2018

Mol Med Report

View full text Add to dashboard Cite

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“…The necessity for a second factor to activate the MSU crystal-induced inflammation was reinforced by a recent in vitro study that evaluated the effect of IL-1β production and IL-1β mRNA expression by macrophages/monocytes on exposure to MSU crystals ( 40 ). Exposure of human macrophages/monocytes to MSU crystals alone did not induce the release of IL-1β, but required the presence of synovial fluid (supernatant and free of cells) from patients with inflammatory arthritis ( 40 ). On fractionation analysis, it was demonstrated that the MSU crystal co-stimulus was contained in the protein fraction but not in the lipidic fraction of synovial fluid ( 40 ).…”

Section: Resultsmentioning

confidence: 99%

“…Exposure of human macrophages/monocytes to MSU crystals alone did not induce the release of IL-1β, but required the presence of synovial fluid (supernatant and free of cells) from patients with inflammatory arthritis ( 40 ). On fractionation analysis, it was demonstrated that the MSU crystal co-stimulus was contained in the protein fraction but not in the lipidic fraction of synovial fluid ( 40 ). However, the protein from synovial fluid in this study acted as a co-stimulatory factor rather than a “primer” because pre-treatment of macrophages/monocytes with synovial fluid did not result in the production of IL-1β ( 40 ).…”

Section: Resultsmentioning

confidence: 99%

Exploring the Link between Uric Acid and Osteoarthritis

Ann

Leung

2017

Front. Med.

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Both gout and osteoarthritis (OA) are common forms of arthritis that inflict a huge burden to an aging population with the increasing prevalence of obesity. Clinicians have long observed the link between these two conditions. In this review, we summarize the evidence from epidemiologic and immunological studies that described the possible relationship between the two conditions. The recent new understanding on monosodium uric acid crystal-induced inflammation has given insight into probable shared pathogenesis pathways for both conditions. We describe the potential therapeutic implications, particularly regarding the possibility of repurposing traditional gout medications for use in OA.

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“…Subsequently, it became clear that purified crystals alone could not induce IL-1but that an additional stimulus was needed in vitro to generate IL-1 (Giamarellos-Bourboulis et al, 2009). Further ex vivo models identified free fatty acids (Joosten et al, 2010) and synovial fluid (SF) proteins (Scanu et al, 2016) as important triggers of IL-1 release.…”

Section: Inflammasome Il-1 and Il-ra In Crystal-induced Arthropathiesmentioning

confidence: 99%

A Brief History of IL-1 and IL-1 Ra in Rheumatology

2017

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The history of what, in 1979, was called interleukin-1 (IL-1), orchestrator of leukocyte inter-communication, began many years before then, initially by the observation of fever induction via the endogenous pyrogen (EP) (1974) and then in rheumatology on the role in tissue destruction in rheumatoid diseases via the induction of collagenase and PGE2 in human synovial cells by a mononuclear cell factor (MCF) (1977). Since then, the family has exploded to presently 11 members as well as many membrane-bound and soluble receptor forms. The discovery of a natural Interleukin-1 receptor antagonist (IL-1Ra) in human biological fluids has highlighted the importance of IL-1 and IL-1Ra in human diseases. Evidence delineating its role in autoinflammatory syndromes and the elucidation of the macromolecular complex referred to as “inflammasome” have been instrumental to our understanding of the link with IL-1. At present, the IL-1blockade as therapeutic approach is crucial for many hereditary autoinflammatory diseases, as well as for adult-onset Still’s disease, crystal-induced arthropathies, certain skin diseases including neutrophil-triggered skin diseases, Behçet’s disease and deficiency of IL-1Ra and other rare fever syndromes. Its role is only marginally important in rheumatoid arthritis and is still under debate with regard to osteoarthritis, type 2 diabetes mellitus, cardiovascular diseases and cancer. This brief historical review focuses on some aspects of IL-1, mainly IL-1β and IL-Ra, in rheumatology. There are many excellent reviews focusing on the IL-1 family in general or with regard to specific diseases or biological discoveries.

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Synovial fluid proteins are required for the induction of interleukin-1β production by monosodium urate crystals

Abstract: This study shows that the SF of inflammatory arthritis patients, including gout patients, contains proteins required for the induction of IL-1β by MSU crystals in macrophages whereas lipids are not involved.

Cited by 14 publications

References 35 publications

Differential miRNAomics of the synovial membrane in knee osteoarthritis induced by bilateral anterior cruciate ligament transection in rats

Differential miRNAomics of the synovial membrane in knee osteoarthritis induced by bilateral anterior cruciate ligament transection in rats

Exploring the Link between Uric Acid and Osteoarthritis

A Brief History of IL-1 and IL-1 Ra in Rheumatology

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