Synovial fluid Waaler-Rose and latex tests.

Huskisson, E.C.; Hart, F. Dudley; Lacey, B. W.

doi:10.1136/ard.30.1.67

Cited by 6 publications

(3 citation statements)

References 5 publications

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“…Figure 2 shows the correlation between SF and serum DAT values in 50 patients with RA. The correlation is fairly strong r = 0.71, P<0-01, and confirms the results of Huskisson et al (1971).…”

Section: Resultssupporting

confidence: 85%

Gold and penicillamine: a proposed mode of action in rheumatoid arthritis, based on synovial fluid analysis.

Pritchard¹,

Nuki²

1978

Annals of the Rheumatic Diseases

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SUMMARY Although in common use, there is still controversy as to the way in which gold and penicillamine act in rheumatoid arthritis (RA).In this study, synovial fluids from 4 groups of patients have been compared: (1) RA patients on gold/penicillamine, (2) RA patients on non-steriodal anti-inflammatory drugs (NSAID) only, (3) osteoarthritis patients, and (4) patients with sero-negative arthropathies. The parameters measured were differential agglutination titre (DAT), total haemolytic complement (CH50), total protein, total white cell count, and immunoglobulins. RA patients on gold/penicillamine have lower synovial DAT levels and higher CH50 levels than RA patients on NSAID only, and total and cryoprecipitable IgM levels very close to those found in the sero-negative joint fluids. The non-specific inflammatory parameters, ie, white cell count and total protein are unchanged after gold/penicillamine therapy.In a second study, the serum DATs of patients in total remission after gold/penicillamine were compared with similar patients on NSAID only. The DAT falls significantly in the former group (P<0 *001), but not in the latter suggesting that fall in DAT is a consequence of therapy rather than remission.The parameters altered by gold/penicillamine in the synovial fluid are those that distinguish RA from non-rheumatoid arthropathies suggesting the drug's primary effect is to render the disease sero-negative. The results support the hypothesis that both drugs have a common mode of action based on their active thiol groups, and that the fall in DAT is due to the reduAtion of the antigenicity of the IgG complexes.

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Section: Resultssupporting

confidence: 85%

Gold and penicillamine: a proposed mode of action in rheumatoid arthritis, based on synovial fluid analysis.

Pritchard¹,

Nuki²

1978

Annals of the Rheumatic Diseases

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“…Any of the factors involved in the immune response that are present at different concentrations in synovial fluid and serum may contribute to the inhibitory properties of rheumatoid synovial fluids revealed by the porcine costal cartilage bioassay. It seems unlikely that rheumatoid factor itself is responsible for the inhibition since it is usually present at similar concentrations in synovial fluid and in serum (Huskisson et al, 1971;Cracchiolo and Barnett 1972;MacSween et al, 1972). It may, however, be indirectly involved since serum complexes of IgGrheumatoid factor differ from those found in joint fluids (Zvaifler, 1973).…”

Section: Synovial Fluidsmentioning

confidence: 99%

Somatomedin activity in synovial fluid from patients with joint diseases.

Coates¹,

Burwell²,

Lloyd-Jones³

et al. 1978

Annals of the Rheumatic Diseases

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SUMmARYThe somatomedin activity in synovial fluids from 50 patients with a variety of joint diseases has been studied and compared with the activity in each of the patient's own serum and a standard reference serum (SRS). The porcine costal cartilage bioassay of Van den Brande and Du Caju (1974a) has been used with the isotopes 3H-thymidine and 35S-sulphate. Synovial fluids from most patients with post-traumatic and post-operative effusions, osteoarthritis and arthritis associated with psoriasis, Reiter's disease, and ankylosing spondylitis stimulated the synthesis of DNA and proteoglycans in cartilage. Synovial fluids from patients with rheumatoid arthritis either had impaired capacity to stimulate DNA synthesis, or they inhibited it; a similar, but less evident pattern was observed for proteoglycan synthesis. Some synovial fluids from patients with miscellaneous synovitides stimulated, while others inhibited cartilage metabolism. It is concluded that the synovial fluid from patients with rheumatoid arthritis and from some patients with miscellaneous synovitides contained an inhibitor(s) to DNA and possibly proteoglycan synthesis.The sera from nearly all the patients stimulated both DNA and proteoglycan synthesis, but the somatomedin potency ratios for serum in terms of SRS were generally less than 1 0. There was a significant inverse correlation between the serum somatomedin potency ratio and the age of the patient.We have previously reported that the somatomedin activity in normal synovial fluid from the knee joint of the ox is lower than that in serum from the same animal; and that the somatomedin activity is related to the total protein and albumin concentrations

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“…Human antigammaglobulins have been detected in high frequency in non-rheumatoid arthritis pleural (Levine, Szanto, Grieble, Bach, and Anderson, 1968) and synovial (Huskisson, Hart, and Lacey, 1971) effusions. The agglutinators, termed rheumatoid factors, were found in a much higher frequency than the 1 to 5 per cent.…”

mentioning

confidence: 99%